桑白皮有效组分对SHR大鼠植物神经及内分泌系统的影响
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- 英文论文题名：Effect of Morus alba L.effective components on SHR rat the endocrine system and the autonomic
- 论文作者：袁培培 ; 克迎迎 ; 杨胜利 ; 李玲玲 ; 王小兰 ; 冯卫生 ; 匡海学 ; 郑晓珂
- 英文论文作者：Yuan Pei-pei ; Ke Ying-ying ; Yang Sheng-li ; Li Ling-ling ; Wang Xiao-lan ; Feng Wei-sheng ; Kuang Hai-xue ; ZHeng Xiao-ke ; Henan University of Traditional Chinese Medicine ; Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment & Chinese Medicine Development of Henan Province ; Heilongjiang University of Chinese Medicine
- 年：2016
- 作者机构：河南中医药大学;呼吸疾病诊疗与新药研发河南省协同创新中心;黑龙江中医药大学;
- 论文关键词：桑白皮 ; SHR ; 植物神经系统 ; 内分泌系统
- 英文论文关键词：Morus alba L. ; SHR ; autonomic nervous system ; endocrine system
- 会议召开时间：2016-09-27
- 会议录名称：中国药学会第十三届青年药学科研成果交流会论文集
- 语种：中文
- 分类号：R285.5
- 学会代码：YYWS
- 会议名称：中国药学会第十三届青年药学科研成果交流会
- 会议地点：中国江西南昌
- 主办单位：中国药学会
- 学会名称：中国药学会
- 页数：9
- 文件大小：1276k
- 原文格式：O
- 会议级别：全国
摘要
目的:探究桑白皮降压有效组分对自发性高血压大鼠植物神经及内分泌系统的调节作用。方法:自发性高血压大鼠(SHR大鼠),雄性,1 2周龄,连续给药7天,采用全自动无创血压测量仪测量给药7天后大鼠血压三次,取三次平均值为最终测量结果。留取血清,采用ELISA法检测多巴胺(DA)、去甲肾上腺素(NE)、17-羟皮质类固醇(17-0HCS)、肾上腺素(EPI)、总三碘甲状原氨酸(TT3)、总甲状腺激素(TT4)、促甲状腺激素(TSH)、促间质细胞激素(ICSH)水平。结果:给予桑白皮水煎液、30%乙醇组分和脂肪油组分后,均能显著降低DA和1 7-0HCS水平(P<0.05,P<0.01);其中,30%乙醇组分组能显著降低NE水平(P<0.05);脂肪油组分能显著减低EPI水平(P<0.05),提示桑白皮有效组分可以明显抑制SHR大鼠交感神经系统的激活状态,缓解高血压所致机体的紊乱;同时,桑白皮水煎液、30%乙醇组分及脂肪油组分组均能极显著降低TT3、TT4、TSH和ICSH水平(P<0.01),提示桑白皮有效组分可以明显改善SHR大鼠内分泌系统的紊乱状态,使机体内环境向稳态发展。结论:桑白皮降低SHR大鼠血压的有效组分为30%组分和脂肪油组分,且其有效组分能显著改善高血压状态下植物神经系统及内分泌系统的紊乱,抑制其激活状态,调节机体内环境的稳态。
Objective:To explore the regulation of Moms alba L.effective components on reducing blood pressure of spontaneously hypertensive rats on autonomic nervous system and the endocrine hypertension.Methods:Spontaneously hypertensive rats(SHR rats),male,12 weeks old,for 7 consecutive days,blood pressure was measured by automatic noninvasive blood pressure measuring three times in rats 7 days after administration,taking an average of three times the final measurement result.Serum specimens by ELISA assay of dopamine(DA),norepinephrine(NE),17-hydroxy corticosteroids(17-OHCS),epinephrine(EPI),triiodo thyroid total triiodothyronine(TT3),total thyroid hormone(TT4),thyroid stimulating hormone(TSH),between stromal cells promote hormone(ICSH) level.Results:After administration of Moms alba L.water extract,30%ethanol component and fatty oil components can significantly reduce DA and17-OHCS level(P<0.05,P<0.01);wherein 30%ethanol group grouping can NE levels decreased significantly(P<0.05);fatty oil components can significantly reduce the EPI levels(P<0.05),prompted Morus alba L.effective components can inhibit the activation of the sympathetic nervous system state SHR rats,relieve hypertension caused by Meanwhile,Moms alba L.water extract,30%ethanol and fatty oil components grouped group can significantly reduce TT3,TT4,TSH and ICSH level(P<0.01),the effective component may prompt Morus alba;body disorder SHR rats significantly improve the state of disorder of the endocrine system,so that the steady development of the internal environment.Conclusion:Moms alba L.reduce blood pressure in SHR rats effectively divided into 30%ethanol and fatty oil components,and it can significantly improve the effective component in hypertension disorder of the autonomic nervous system and the endocrine system,inhibiting its activation status regulate internal environment homeostasis.
Objective:To explore the regulation of Moms alba L.effective components on reducing blood pressure of spontaneously hypertensive rats on autonomic nervous system and the endocrine hypertension.Methods:Spontaneously hypertensive rats(SHR rats),male,12 weeks old,for 7 consecutive days,blood pressure was measured by automatic noninvasive blood pressure measuring three times in rats 7 days after administration,taking an average of three times the final measurement result.Serum specimens by ELISA assay of dopamine(DA),norepinephrine(NE),17-hydroxy corticosteroids(17-OHCS),epinephrine(EPI),triiodo thyroid total triiodothyronine(TT3),total thyroid hormone(TT4),thyroid stimulating hormone(TSH),between stromal cells promote hormone(ICSH) level.Results:After administration of Moms alba L.water extract,30%ethanol component and fatty oil components can significantly reduce DA and17-OHCS level(P<0.05,P<0.01);wherein 30%ethanol group grouping can NE levels decreased significantly(P<0.05);fatty oil components can significantly reduce the EPI levels(P<0.05),prompted Morus alba L.effective components can inhibit the activation of the sympathetic nervous system state SHR rats,relieve hypertension caused by Meanwhile,Moms alba L.water extract,30%ethanol and fatty oil components grouped group can significantly reduce TT3,TT4,TSH and ICSH level(P<0.01),the effective component may prompt Morus alba;body disorder SHR rats significantly improve the state of disorder of the endocrine system,so that the steady development of the internal environment.Conclusion:Moms alba L.reduce blood pressure in SHR rats effectively divided into 30%ethanol and fatty oil components,and it can significantly improve the effective component in hypertension disorder of the autonomic nervous system and the endocrine system,inhibiting its activation status regulate internal environment homeostasis.
引文
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[4]SliwaK,Stewart S,Gersh BJ.Hypertension:a global perspective[J].Circulation,2011,123(24):2892-2896.
[5]Zhou JY,Mo ZX,Zhou SW.Effect of rhynchophylline on central neurotransmitter levels in amphetamine-induced conditioned place preference rat brain[J].Fitoterapia,2010,81(7):844-848.
[6]于首元.牛黄降压丸的降压疗效及其对交感神经活性的影响[J].中国中药杂志,2007,32(2):172-175.
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[10]朴淑娟,曲戈霞,邱峰.桑白皮水提物中化学成分的研究[J].中国药物化学杂志,2006,16(1):40-45.
[11]张志明,梁静珏.桑树的黄酮类化学成分及药理研究进展[J].海峡药学,2006,18(1):1-6.
[12]Wang,R.,Lu,X.C.,Wang,Y.W.,2002.Anti-inflammatory activity of the extracts of Ramulus mori,Journal of Wuhan Botanical Research20,467-469.
[13]El-Beshbishy,H.A.,Singab,A.N.,Sinkkonen,J.,Pihlaja,K.,2006.Hypolipidemic and anti-oxidant effects of Moras alba L.(Egyptian mulberry)root bark fractions supplementation in cholesterol-fed rats.Life Sciences 78,2724-2733.
[14]Zhang,M.,Chen,M.,Zhang,H.Q.,Sun,S.,Xia,B.,Wu,F.H.,2009.In vivo hypoglycemic effects of phenolics from the root bark of Moras alba.Fitoterapia 80,475-477.
[15]Park,K.M.,You,J.S.,Lee,H.Y.,Baek,N.I.,Hwang,J.K.,2003.Kuwanon G:an antibacterial agent from the root bark of Moras alba against oral pathogens.Journal of Ethnopharmacology 84,181-185.
[16]Nam,S.Y.,Yi,H.K.,Lee,J.C.,Kim,J.C.,Song,C.H.,Park,J.W.,Lee,D.Y.,Kim,J.S.,Hwang,P.H.,2002.Cortex Mori extract induces cancer cell apoptosis through inhibition of microtubule assembly.Archives of Pharmacal Research 25,191-196.
[17]Du,J.,He,Z.D.,Jiang,R.W.,Ye,W.C.,Xu,H.X.,But,P.P.,2003.Antiviral flavonoids from the root bark of Moras alba L.Phytochemistry 62,1235-1238.
[18]崔珏,李超,姜中生.桑白皮总黄酮的抗氧化与镇痛活性研究[J].食品科学,2011,32(23):281-284.
[19]韦嫒嫒,徐峰,陈晓伟,等.桑白皮黄酮平喘作用实验研究[J].时珍国医国药,2009,20(11):2743-2745.
[20]冯冰虹,赵宇红,黄建华.桑白皮的有效成分筛选及其药理学研究[J].中药材,2004,27(3):204.205.
[21]耿鹏,石倩,杨洋等.桑白皮生物碱与绿茶茶多酚的联合降糖作用[J].南开大学学报:自然科学版,2011,4(3):76-79.
[22]汝海龙,林国华,沈礼.桑白皮乙酸乙酯提取物的舒血管作用及其机制初探[J].基础医学,2012,32(5):321.324.
[23]袁培培,克迎迎,王小兰,冯卫生,郑晓珂.桑白皮拆分组分对正常大鼠植物神经及内分泌系统的影响[A].第一届《药学学报》药学前沿论坛暨2015年中国药学会中药与天然药物专业委员会会议论文摘要集[C].《药学学报》编委会、中国药学会中药与天然药物专业委员会:,2015:1.
[24]汪道文,赵华月.植物神经系统与心血管疾病的研究进展[J].心血管病学进展,1995,05:257-260.
[25]李振宇,王秋红,杨炳友,舒尊鹏,匡海学.基于对植物神经系统相关指标影响的洋金花各化学拆分组分的药性研究[J].中国实验方剂学杂志,2016,09:86-90.
[26]李连珍,刘小青,崔一然,张冰.辛热示例药对正常大鼠基础代谢及内分泌系统的影响[J].中国中医药信息杂志,2011,03:49-51.
[27]张凌燕,温庆城,张志文.神经系统与内分泌系统的相互影响与协同作用[J].生物学通报,2006,07:24-25.
[28]李红武.长时间不同运动应激导致大鼠神经内分泌系统变化机制的研究[D].华东师范大学,2003.
[2]杨志敏.徐贵成主任医师辨治高血压病用药经验总结[D].北京:北京中医药大学,2010.
[3]Lewington S,Clarke R,Qizilbash N,et al.Age specific relevance of usual blood pressure to vascular mortality:a meta analysis of individual data for one million adults in 61 prospective studies[J].Lancet,2002,360(9349):637-646.
[4]SliwaK,Stewart S,Gersh BJ.Hypertension:a global perspective[J].Circulation,2011,123(24):2892-2896.
[5]Zhou JY,Mo ZX,Zhou SW.Effect of rhynchophylline on central neurotransmitter levels in amphetamine-induced conditioned place preference rat brain[J].Fitoterapia,2010,81(7):844-848.
[6]于首元.牛黄降压丸的降压疗效及其对交感神经活性的影响[J].中国中药杂志,2007,32(2):172-175.
[7]周明.苦马兰总黄酮苷的降压作用及机理[J].湖南中医药导报,1997,3(5):21-23.
[8]中华人民共和国卫生部药典委员会.中华人民共和国药典[S].一部.北京:化学工业出版社,2010.
[9]王瑾,张会敏,石俊英.桑自皮黄酮类化学成分研究进展[J].齐鲁药事,2012 31(7):420-422.
[10]朴淑娟,曲戈霞,邱峰.桑白皮水提物中化学成分的研究[J].中国药物化学杂志,2006,16(1):40-45.
[11]张志明,梁静珏.桑树的黄酮类化学成分及药理研究进展[J].海峡药学,2006,18(1):1-6.
[12]Wang,R.,Lu,X.C.,Wang,Y.W.,2002.Anti-inflammatory activity of the extracts of Ramulus mori,Journal of Wuhan Botanical Research20,467-469.
[13]El-Beshbishy,H.A.,Singab,A.N.,Sinkkonen,J.,Pihlaja,K.,2006.Hypolipidemic and anti-oxidant effects of Moras alba L.(Egyptian mulberry)root bark fractions supplementation in cholesterol-fed rats.Life Sciences 78,2724-2733.
[14]Zhang,M.,Chen,M.,Zhang,H.Q.,Sun,S.,Xia,B.,Wu,F.H.,2009.In vivo hypoglycemic effects of phenolics from the root bark of Moras alba.Fitoterapia 80,475-477.
[15]Park,K.M.,You,J.S.,Lee,H.Y.,Baek,N.I.,Hwang,J.K.,2003.Kuwanon G:an antibacterial agent from the root bark of Moras alba against oral pathogens.Journal of Ethnopharmacology 84,181-185.
[16]Nam,S.Y.,Yi,H.K.,Lee,J.C.,Kim,J.C.,Song,C.H.,Park,J.W.,Lee,D.Y.,Kim,J.S.,Hwang,P.H.,2002.Cortex Mori extract induces cancer cell apoptosis through inhibition of microtubule assembly.Archives of Pharmacal Research 25,191-196.
[17]Du,J.,He,Z.D.,Jiang,R.W.,Ye,W.C.,Xu,H.X.,But,P.P.,2003.Antiviral flavonoids from the root bark of Moras alba L.Phytochemistry 62,1235-1238.
[18]崔珏,李超,姜中生.桑白皮总黄酮的抗氧化与镇痛活性研究[J].食品科学,2011,32(23):281-284.
[19]韦嫒嫒,徐峰,陈晓伟,等.桑白皮黄酮平喘作用实验研究[J].时珍国医国药,2009,20(11):2743-2745.
[20]冯冰虹,赵宇红,黄建华.桑白皮的有效成分筛选及其药理学研究[J].中药材,2004,27(3):204.205.
[21]耿鹏,石倩,杨洋等.桑白皮生物碱与绿茶茶多酚的联合降糖作用[J].南开大学学报:自然科学版,2011,4(3):76-79.
[22]汝海龙,林国华,沈礼.桑白皮乙酸乙酯提取物的舒血管作用及其机制初探[J].基础医学,2012,32(5):321.324.
[23]袁培培,克迎迎,王小兰,冯卫生,郑晓珂.桑白皮拆分组分对正常大鼠植物神经及内分泌系统的影响[A].第一届《药学学报》药学前沿论坛暨2015年中国药学会中药与天然药物专业委员会会议论文摘要集[C].《药学学报》编委会、中国药学会中药与天然药物专业委员会:,2015:1.
[24]汪道文,赵华月.植物神经系统与心血管疾病的研究进展[J].心血管病学进展,1995,05:257-260.
[25]李振宇,王秋红,杨炳友,舒尊鹏,匡海学.基于对植物神经系统相关指标影响的洋金花各化学拆分组分的药性研究[J].中国实验方剂学杂志,2016,09:86-90.
[26]李连珍,刘小青,崔一然,张冰.辛热示例药对正常大鼠基础代谢及内分泌系统的影响[J].中国中医药信息杂志,2011,03:49-51.
[27]张凌燕,温庆城,张志文.神经系统与内分泌系统的相互影响与协同作用[J].生物学通报,2006,07:24-25.
[28]李红武.长时间不同运动应激导致大鼠神经内分泌系统变化机制的研究[D].华东师范大学,2003.