芍药与牡丹组间杂种‘Bartzella'的微繁殖技术研究
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- 英文论文题名：Studies on the Micropropagation of Intersectional Hybrids between Herbaceous and Tree Peonies ‘Bartzella’ in Paeonia
- 论文作者：李刘泽木 ; 成仿云 ; 钟原 ; 文书生 ; 王新 ; 黄弄璋
- 英文论文作者：LI Liu-zemu ; CHENG Fang-yun ; ZHONG Yuan ; WEN Shu-sheng ; WANG Xin ; HUANG Nong-zhang ; Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding ; National Engineering Research Center for Floriculture ; Beijing Laboratory of Urban and Rural Ecological Environment and College of Landscape Architecture ; Beijing Forestry University
- 年：2016
- 作者机构：花卉种质创新与分子育种北京市重点实验室国家花卉工程技术研究中心城乡生态环境北京实验室园林学院北京林业大学;
- 论文关键词：芍药与牡丹 ; 组间杂种 ; ‘Bartzella’ ; 微繁殖
- 英文论文关键词：Peonies(Paeonia) ; Intersectional hybrids ; ‘Bartzella’ ; Micropropagation
- 会议召开时间：2016-07-19
- 会议录名称：中国观赏园艺研究进展2016
- 语种：中文
- 分类号：S682.12;S685.11
- 学会代码：EGYP
- 会议名称：2016年中国观赏园艺学术研讨会
- 会议地点：中国湖南长沙
- 主办单位：中国园艺学会观赏园艺专业委员会、国家花卉工程技术研究中心
- 学会名称：中国园艺学会
- 页数：7
- 文件大小：725k
- 原文格式：O
- 会议级别：全国
摘要
本文以芍药与牡丹组间杂种(伊藤杂种)‘Bartzella'的鳞芽为外植体,初步建立了其微繁殖体系。主要结果包括:①‘Bartzella'的最佳取材时间为3月下旬;②WPM培养基中Ca(NO_3)_2浓度提高至原浓度的4倍(2224mg·L~(-1)),可显著促进组培苗的生长和增殖;添加有机物水解酪蛋白0.25g·L~(-1)也可在一定程度上提高组培苗增殖率。③生根培养前,将无根苗先接种于培养基1/2 MS中复壮培养10天,然后转入1/2 MS+腐胺1.0mg·L~(-1)+IBA 1.0mg·L~(-1)培养基中诱导根原基,冷处理12天后转常温黑暗环境下诱导培养30天,最后在根形成培养基1/2MS+活性炭4.0g·L~(-1)中培养20天,生根率可达75.51%。④生根质量对移栽成活率影响显著,根数多于3条且愈伤组织小的生根苗移栽成活率高达73.58%。
The in-vitro cultured shoots of intersectional hybrids between herbaceous and tree peonies ' Bartzella' were used to develop a protocol for micro-propagation.The results showed that:①The optimal sampling time of ' Bartzella' was late March.②Modification of WPM basal medium on fourfold Ca(NO_3)_2 and adding 0.25 g · L~(-1) casein acid hydrolysate were effective on the improving of multiplication and growth of ' Bartzella'.③For optimal rooting,the shoots were cultured on 1/2MS for 10 days,then cultured on 1/2 MS + putrescine 1.0mg · L~(-1) + IBA 1.0mg · L~(-1) for 30 days to induce rooting after a cold treatment(4℃)for 12 days,and then transferred onto root formation medium(1/2MS + Activated carbon 4.0mg · L~(-1))for20 days,so that 75.51%rooting rate was achieved finally.④The rooting quality had significantly effect on survival of in-vitro rooted plantlets in ' Bartzella'.Plantlets with root number more than three and less callus survived 73.58%.
The in-vitro cultured shoots of intersectional hybrids between herbaceous and tree peonies ' Bartzella' were used to develop a protocol for micro-propagation.The results showed that:①The optimal sampling time of ' Bartzella' was late March.②Modification of WPM basal medium on fourfold Ca(NO_3)_2 and adding 0.25 g · L~(-1) casein acid hydrolysate were effective on the improving of multiplication and growth of ' Bartzella'.③For optimal rooting,the shoots were cultured on 1/2MS for 10 days,then cultured on 1/2 MS + putrescine 1.0mg · L~(-1) + IBA 1.0mg · L~(-1) for 30 days to induce rooting after a cold treatment(4℃)for 12 days,and then transferred onto root formation medium(1/2MS + Activated carbon 4.0mg · L~(-1))for20 days,so that 75.51%rooting rate was achieved finally.④The rooting quality had significantly effect on survival of in-vitro rooted plantlets in ' Bartzella'.Plantlets with root number more than three and less callus survived 73.58%.
引文
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2.Bouza L,Jacques M,Sotta B,et al.1994.The reactivation of tree peony(Paeonia suffruticosa Andr.)vitroplants by chilling is correlated with modifications of abscisic acid,auxin and cytokinin levels[J].Plant Science,93:153-160.
3.Beruto M,Lanteri L,Portogallo C.2004.Micropropagation of tree peony(Paeonia suffruticosa)[J].Plant Cell Tissue and Organ Culture,79(2):249-255.
4.Beruto M,Curir P.2007.In vitro culture of tree peony through axillary budding//Jain S M,Haggman H.Protocols for mieropropagation of woody trees and fruits[M],Springer;477-497.
5.陈正华.1986.木本植物组织培养及其应用[M].北京:高等教育出版社.
6.Hameed M A,Reid J B,Rowe R N.1987.Root confinement,and its effects on the water relations,growth and assimilate partitioning of tomato(Lycopersicon escutentum Mill.)[J].Annals of Botany,59:685-692.
7.Hosoki T,Ando M,Kubara T,Hamada M,Itami M.1989.In vitro propagation of herbaceous peony(Paeonia lactiflora Pall.)by a longitudinal shoot-split method[J].Plant Cell Reports,8(4):243-246.
8.贾梦雪,徐瑾,叶香娟,等.2013.春石斛优良品种‘森禾2006'组培快繁体系的建立[J].植物生理学报,49(12):1363-1367.
9.贾文庆,徐小博,刘会超,等.2013.牡丹‘乌龙捧盛'组培苗生根及生根解剖学研究[J].林业科学研究,26(4):516-520.
10.孔祥生,张妙霞.1998.牡丹离体快繁技术研究[J].北方园艺.3(4):87-89
11.李萍.2007.牡丹组培快繁技术的研究[D].北京林业大学.
12.Li P,Cheng F Y.2008.Basal Medium with Modified Calcium Source and Other Factors Influence on Shoots Culture of Tree Peony[J].Acta Hortic,766:383-389.
13.Page M.1997.The gardener's guide to growingpeonies[M].David&Charles,Timber Press.
14.Page M.2005.Paeonia Itoh hybrids.Plantsman;New Series,4(1):36.
15.潘瑞炽.2001.植物生理学[M].北京:高等教育出版社.
16.庞利铮,成仿云.2011.牡丹与芍药组间远缘杂种(伊藤杂种)的嫁接繁殖[J].中国花卉园艺,(22):30-32.
17.邱金梅.2010.牡丹微繁殖技术的研究[D].北京:北京林业大学硕士论文.
18.Qin L,Cheng F Y,Zhong Y,P Gao,H Yu.2012.Callus development in tree peonies(Paeonia Sect.Moutan);influence of genotype,explant development stage and position,and plant growth regulators[J].Propagation of Ornamental Plants,12(2):117-126.
19.瞿素萍,熊丽,李树发等.2006.云南野生早花象牙参叶片再生体系的建立[J].园艺学报,(02):441-444.
20.沈海龙.2009.树木组织培养微枝试管外生根育苗技术[M].北京:中国林业出版社.
21.Teixeira Da Silva J A,Shen M,Yu X.2012.Tissue culture and micropropagation of tree peony(Paeonia suffhiticosa Andr.)[J].Journal of Crop Science and Biotechnology,15(3):159-168.
22.文书生,成仿云,钟原等.2016.‘正午'牡丹微繁殖体系的建立[J].植物科学学报.(01):1-8.
23.Whysall.2006.Pursuit of the perfect peony,an exciting new breed of peony is being made available this spring,thanks to a groundbreaking propagation technique developed by Quebec company[J].Planteck Biotechnologies.
24.杨世海,刘晓峰,果德安,等.2006.不同附加物对甘草愈伤组织培养中黄酮类化合物形成的影响[J].中国药学杂志,41(2):96-99.
25.杨增海.1987.园艺植物组织培养[M].北京:中国农业出版社.
26.张倩,王华芳.2012.牡丹试管苗生根与移栽技术研究进展[J].园艺学报,(09):1819-1828.
2.Bouza L,Jacques M,Sotta B,et al.1994.The reactivation of tree peony(Paeonia suffruticosa Andr.)vitroplants by chilling is correlated with modifications of abscisic acid,auxin and cytokinin levels[J].Plant Science,93:153-160.
3.Beruto M,Lanteri L,Portogallo C.2004.Micropropagation of tree peony(Paeonia suffruticosa)[J].Plant Cell Tissue and Organ Culture,79(2):249-255.
4.Beruto M,Curir P.2007.In vitro culture of tree peony through axillary budding//Jain S M,Haggman H.Protocols for mieropropagation of woody trees and fruits[M],Springer;477-497.
5.陈正华.1986.木本植物组织培养及其应用[M].北京:高等教育出版社.
6.Hameed M A,Reid J B,Rowe R N.1987.Root confinement,and its effects on the water relations,growth and assimilate partitioning of tomato(Lycopersicon escutentum Mill.)[J].Annals of Botany,59:685-692.
7.Hosoki T,Ando M,Kubara T,Hamada M,Itami M.1989.In vitro propagation of herbaceous peony(Paeonia lactiflora Pall.)by a longitudinal shoot-split method[J].Plant Cell Reports,8(4):243-246.
8.贾梦雪,徐瑾,叶香娟,等.2013.春石斛优良品种‘森禾2006'组培快繁体系的建立[J].植物生理学报,49(12):1363-1367.
9.贾文庆,徐小博,刘会超,等.2013.牡丹‘乌龙捧盛'组培苗生根及生根解剖学研究[J].林业科学研究,26(4):516-520.
10.孔祥生,张妙霞.1998.牡丹离体快繁技术研究[J].北方园艺.3(4):87-89
11.李萍.2007.牡丹组培快繁技术的研究[D].北京林业大学.
12.Li P,Cheng F Y.2008.Basal Medium with Modified Calcium Source and Other Factors Influence on Shoots Culture of Tree Peony[J].Acta Hortic,766:383-389.
13.Page M.1997.The gardener's guide to growingpeonies[M].David&Charles,Timber Press.
14.Page M.2005.Paeonia Itoh hybrids.Plantsman;New Series,4(1):36.
15.潘瑞炽.2001.植物生理学[M].北京:高等教育出版社.
16.庞利铮,成仿云.2011.牡丹与芍药组间远缘杂种(伊藤杂种)的嫁接繁殖[J].中国花卉园艺,(22):30-32.
17.邱金梅.2010.牡丹微繁殖技术的研究[D].北京:北京林业大学硕士论文.
18.Qin L,Cheng F Y,Zhong Y,P Gao,H Yu.2012.Callus development in tree peonies(Paeonia Sect.Moutan);influence of genotype,explant development stage and position,and plant growth regulators[J].Propagation of Ornamental Plants,12(2):117-126.
19.瞿素萍,熊丽,李树发等.2006.云南野生早花象牙参叶片再生体系的建立[J].园艺学报,(02):441-444.
20.沈海龙.2009.树木组织培养微枝试管外生根育苗技术[M].北京:中国林业出版社.
21.Teixeira Da Silva J A,Shen M,Yu X.2012.Tissue culture and micropropagation of tree peony(Paeonia suffhiticosa Andr.)[J].Journal of Crop Science and Biotechnology,15(3):159-168.
22.文书生,成仿云,钟原等.2016.‘正午'牡丹微繁殖体系的建立[J].植物科学学报.(01):1-8.
23.Whysall.2006.Pursuit of the perfect peony,an exciting new breed of peony is being made available this spring,thanks to a groundbreaking propagation technique developed by Quebec company[J].Planteck Biotechnologies.
24.杨世海,刘晓峰,果德安,等.2006.不同附加物对甘草愈伤组织培养中黄酮类化合物形成的影响[J].中国药学杂志,41(2):96-99.
25.杨增海.1987.园艺植物组织培养[M].北京:中国农业出版社.
26.张倩,王华芳.2012.牡丹试管苗生根与移栽技术研究进展[J].园艺学报,(09):1819-1828.