应用鱼类生物标志物和IBRv2监测与评价泉州湾石油类污染生物效应状况
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- 英文论文题名：Applying fish biomarkers and IBRv2 for monitoring and assessing biological effects of petroleum hydrocarbon pollution in Quanzhou Bay
- 论文作者：薄军 ; 郑榕辉 ; 陈晔 ; 洪幅坤 ; 谢晴 ; 陈金民 ; 邝伟明 ; 陈荣 ; 张玉生
- 英文论文作者：BO Jun ; ZHENG Rong-hui ; CHEN Ye ; HONG Fu-kun ; XIE Qing ; CHEN Jin-min ; KUANG Wei-ming ; CHEN Rong ; ZHANG Yu-sheng ; Third Institute of Oceanography ; State Oceanic Administration ; College of the Environment and Ecology ; Xiamen University
- 年：2016
- 作者机构：国家海洋局第三海洋研究所;厦门大学环境与生态学院;
- 论文关键词：细胞色素P4501A ; 生物标志物 ; 石油类污染 ; 野外监测 ; 二代综合生物标志物响应指数法
- 英文论文关键词：Cytochrome P4501A ; Biomarkers ; Petroleum hydrocarbon pollution ; Field monitoring ; Integrated Biological Responses version 2
- 会议召开时间：2016-11-11
- 会议录名称：福建省海洋学会2016年学术年会论文摘要集
- 语种：中文
- 分类号：X835;X55
- 学会代码：FJHY
- 会议名称：福建省海洋学会2016年学术年会
- 会议地点：中国福建厦门
- 主办单位：福建省海洋学会、福建省水产研究所
- 学会名称：福建省海洋学会
- 页数：2
- 文件大小：67k
- 原文格式：O
- 会议级别：地方
摘要
本研究以褐菖鲉细胞色素P4501A四种生物标志物,即鳃丝EROD活性、肝EROD活性、肝CYP1A mRNA和肝CYP1A蛋白为指标,在泉州湾海域选择6个站位(包括1个对照站位),开展了为期2周的野外监测实验,研究了上述生物标志物的变化特征以及它们同表层海水中石油类含量和沉积物物中16种优先监控的PAHs含量之间的相关性,并利用新一代综合生物标志物响应指数法(Integrated Biologic al Responses version 2,IBRv2)对监测站位石油类和PAHs污染效应进行了评价。结果显示,不同站位的上述四种生物标志物变化趋势基本一致;生物标志物的变化与相应站位表层水中石油类含量及沉积物中PAHs总含量相吻合;IBRv2分析结果表明,各监测站位IBRv2指数介于1.5~4.2之间,其中2号站位环境污染压力最大,4号站位最小。在复杂的海洋污染条件下,采用CYP1A多种生物标志物监测技术能够更加全面反映石油类等污染及其生物效应状况。
In the present study,rock fish Sebastiscus marmoratus was chosen as a sentinel animal,and its cytochrome P4501A(CYP1A) biomarkers,namely gill filament and hepatic EROD activities,CYP1 A mRNA and its protein expressions were used as biomarkers to monitor the effects of petroleum hydrocarbon pollution in Quanzhou Bay,Fujian Province.The field monitoring experiment was conducted for 2 weeks in 6 sites including a reference site.The expression patterns of the CYP1 A biomarkers and the correlation of the biomarkers with the contents of petroleum hydrocarbons in the surface water and polycyclic aromatic hydrocarbons(PAHs) in sediment were studied,respectively.Moreover,a novel method for ecological risk assessment named "Integrated Biological Responses version 2(IBRv2)" was used to assess the environmental pollution stress on the S.marmoratus.Compared to the fish caged in the reference site,the gill filament and hepatic EROD activities,CYP1 A mRNA and its protein expressions were significantly elevated in fish from the sites where was much more polluted by petroleum hydrocarbons and PAHs,especially by the high molecular weight PAHs.The IBRv2 index of the 6sites varied between 1.5 and 4.2,and the site 2 had the highest environmental stress while the site4 had the lowest.The present results show that the integrated biomarker of CYP1 A of S.marmoratus is a good approach for monitoring petroleum hydrocarbon pollution and assessing its biological effects in marine coastal environment.
In the present study,rock fish Sebastiscus marmoratus was chosen as a sentinel animal,and its cytochrome P4501A(CYP1A) biomarkers,namely gill filament and hepatic EROD activities,CYP1 A mRNA and its protein expressions were used as biomarkers to monitor the effects of petroleum hydrocarbon pollution in Quanzhou Bay,Fujian Province.The field monitoring experiment was conducted for 2 weeks in 6 sites including a reference site.The expression patterns of the CYP1 A biomarkers and the correlation of the biomarkers with the contents of petroleum hydrocarbons in the surface water and polycyclic aromatic hydrocarbons(PAHs) in sediment were studied,respectively.Moreover,a novel method for ecological risk assessment named "Integrated Biological Responses version 2(IBRv2)" was used to assess the environmental pollution stress on the S.marmoratus.Compared to the fish caged in the reference site,the gill filament and hepatic EROD activities,CYP1 A mRNA and its protein expressions were significantly elevated in fish from the sites where was much more polluted by petroleum hydrocarbons and PAHs,especially by the high molecular weight PAHs.The IBRv2 index of the 6sites varied between 1.5 and 4.2,and the site 2 had the highest environmental stress while the site4 had the lowest.The present results show that the integrated biomarker of CYP1 A of S.marmoratus is a good approach for monitoring petroleum hydrocarbon pollution and assessing its biological effects in marine coastal environment.
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