琼脂硫酸酯酶的发酵优化及中试放大
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- 英文论文题名：Optimization and scale up of fermentation process for agar sulfatase production
- 论文作者：田友明 ; 孙元亨 ; 肖安风
- 英文论文作者：Tian Youming ; Sun Yuanheng ; Xiao Anfeng ; College of Food and Biological Engineering ; Jimei University ; Key Laboratory of Food Microbiology and Enzyme Engineering of Fujian Province ; Fujian Provincial Engineering TechnologyResearch Center of marine functional food ; Xiamen Key Laboratory of Marine Functional Food
- 年：2016
- 作者机构：集美大学食品与生物工程学院;福建省食品微生物与酶工程重点实验室;福建省海洋功能食品工程技术研究中心;厦门市海洋功能食品重点实验室;
- 论文关键词：琼脂硫酸酯酶 ; 发酵 ; 中试放大 ; 酶活力
- 英文论文关键词：agar sulfatase ; fermetation ; scale up ; enzyme activity
- 会议召开时间：2016-11-09
- 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
- 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
- 语种：中文
- 分类号：TQ925
- 学会代码：ZGSP
- 会议名称：中国食品科学技术学会第十三届年会
- 会议地点：中国北京
- 主办单位：中国食品科学技术学会
- 学会名称：中国食品科学技术学会
- 页数：2
- 文件大小：102k
- 原文格式：O
- 会议级别：全国
摘要
本试验以实验室克隆表达的产琼脂硫酸酯酶的重组大肠杆菌(E.coli-ars)为研究对象,首先在摇瓶中对其诱导条件进行单因素优化,得到最佳条件为:诱导温度21℃,诱导时间24h,诱导剂α-乳糖4g/L,诱导初始菌浓度(OD_(50))0.6;然后在5 L罐中对菌株进行发酵,在发酵过程中进行补料试验,结果表明补料提高了生物量,但是对酶活力没有促进效果。最后在摇瓶及5 L罐发酵基础上,分别进行20 L和200 L发酵罐的中试放大实验,结果表明,20 L罐中最大酶活力为157.5 U/mL,200 L罐中的最大酶活力为110.4 U/mL。
This experiment regarded the strain of recombinant Escherichia coli(E.coli-ars) which produced recombinant agar sulfatase as the research object.First,the optimization of induction conditions in shaking flask were implemented by single factor experiment to enhance enzyme production,and then the optimal induction conditions were obtained:induction temperature 21℃,induction time 24 h,inducing agent α-lactose 4g/L,inducing initial biomass(OD_(600)) 0.6.Thereafter,the fermented experiment of E.coli-ars in 5 L bioreactor was carried out.Research results indicated that additional medium significantly improved cell density,while it had no effect on enzyme accumulation.Finally,the pilot scale test of 20 L and 200 L were conducted on the basis of the optimized fermentation processes of shaking flask and 5 L bioreactor.The enzyme activity of the agar sulfatase in 20 L and 200 L bioreactor reached 157.5 U/mL and 110.4 U/mL,respectively.
This experiment regarded the strain of recombinant Escherichia coli(E.coli-ars) which produced recombinant agar sulfatase as the research object.First,the optimization of induction conditions in shaking flask were implemented by single factor experiment to enhance enzyme production,and then the optimal induction conditions were obtained:induction temperature 21℃,induction time 24 h,inducing agent α-lactose 4g/L,inducing initial biomass(OD_(600)) 0.6.Thereafter,the fermented experiment of E.coli-ars in 5 L bioreactor was carried out.Research results indicated that additional medium significantly improved cell density,while it had no effect on enzyme accumulation.Finally,the pilot scale test of 20 L and 200 L were conducted on the basis of the optimized fermentation processes of shaking flask and 5 L bioreactor.The enzyme activity of the agar sulfatase in 20 L and 200 L bioreactor reached 157.5 U/mL and 110.4 U/mL,respectively.
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