食鹿角菜假交替单胞菌ASY5产κ-卡拉胶酶发酵条件优化
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- 英文论文题名：Fermentation optimationofK-carrageenase from pseudoalteromonas carrageenovora ASY5
- 论文作者：李佳佳 ; 朱艳冰 ; 倪辉 ; 蔡慧农 ; 肖安风
- 英文论文作者：Li Jiajia ; Zhu Yanbing ; Ni Hui ; Cai Huinong ; Xiao Anfeng ; College of Food and Biological Engineering ; Jimei University ; Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering ; Fujian Provincial Engineering Technology Research Center of Marine Functional Food ; Xiamen Key Laboratory of Marine Functional Food
- 年：2016
- 作者机构：集美大学食品与生物工程学院;福建省食品微生物与酶工程重点实验室;福建省海洋功能食品工程技术研究中心;厦门市海洋功能食品重点实验室;
- 论文关键词：κ-卡拉胶酶 ; 食鹿角菜假交替单胞菌ASY5 ; 发酵条件优化
- 英文论文关键词：κ-carrageenase ; pseudoalteromonas carrageenovora ASY5 ; fermentation optimation
- 会议召开时间：2016-11-09
- 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
- 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
- 语种：中文
- 分类号：TQ925
- 学会代码：ZGSP
- 会议名称：中国食品科学技术学会第十三届年会
- 会议地点：中国北京
- 主办单位：中国食品科学技术学会
- 学会名称：中国食品科学技术学会
- 页数：2
- 文件大小：110k
- 原文格式：O
- 会议级别：全国
摘要
本实验以红树林土壤腐叶中筛选得到的一株产卡拉胶酶的菌株ASY5为研究对象,对其发酵培养基及培养条件进行单因素优化,得到最佳的条件为:卡拉胶5g/L,胰蛋白胨5g/L,NaCl 20g/L,CaCl_2 0.2g/L,NaH_2PO_4·2H_2O 1.3g/L,Na_2HPO_4·12H_2O 3.82g/L,温度18℃,培养基初始pH为6.5,接种量2%,装液量70mL。经过优化,生物量最大达到1.4,比初始条件下的生物量(0.776)提高80.4%;卡拉胶酶活力最大达到40.8 U/mL,较优化前的酶活力26.8 U/mL提高52.2%。然后对5 L罐的发酵条件进行优化,结果表明,提高转速能促进产酶速率并缩短发酵时间,其中转速为280 rpm时较佳,补料及控制pH对酶活力没有促进作用;最后在摇瓶及5 L罐发酵基础上,进行20 L、75 L和200 L的中试放大实验,结果表明,20 L、75 L和200 L罐中的卡拉胶酶活力最大分别为33.9 U/mL,34.7 U/mL和36.1 U/mL。其中200 L罐中的最大酶活力为摇瓶中最大酶活力的88.5%,为5 L罐中最大酶活力的85.7%。
In this study,a K-carrageenase-producing bacterium from Pseudoalteromonascarrageenovora ASY5 was isolate from mangrove soli leaf by our laboratory.At first,fermentation mediums and culture conditions in shaking flask were carried out by single factor experiment to enhance enzyme production.After optimization,the optimal medium composition and culture conditions were obtained with carrageenan 5g/L,tryptone 5g/L,NaCl 20g/L,CaCl_2 0.2g/L,NaH_2PO_4 · 2H_2O 1.3g/L,Na_2HPO_4 · 12H_2O 3.82g/L,tempreture 18℃,initial pH 6.5,inculation volume 2%,liquid volume 70 mL.Meanwhile,the three mediums were reduced comparing with the basal mediums.The biomass reached 1.4,which was 80.4%over initial biomass(0.776).Carrageenase activity reached 40.8 U/mL,and enzyme activity was 52.2%higher than initial carrageenase activity(26.8 U/mL).Then,the experiment of culture conditions in 5 L bioreactor was carried out.The results showed that high rotation speed can promote the rate of producing enzyme and short the ferment time,280 rpm was the optimal rotation speed,and that additional medium and control pH had not an obvious effect on improving enzyme production.Finally,the pilot enlarge experiment of 20 L,75 L and 200 L were conducted based on the ferment results of shaking flask and 5 L bioreactor.The maximum carrageenase activitives of 20 L,75 L and 200 L bioreactor reached 33.9 U/mL,34.7 U/mL and36.1 U/mL,respectively.And the maximum enzyme activity of 200 L bioreactor was 88.5%of the maximum enzyme activity of shaking flask,and 85.7%of the enzyme activity of 5 L bioreactor.
In this study,a K-carrageenase-producing bacterium from Pseudoalteromonascarrageenovora ASY5 was isolate from mangrove soli leaf by our laboratory.At first,fermentation mediums and culture conditions in shaking flask were carried out by single factor experiment to enhance enzyme production.After optimization,the optimal medium composition and culture conditions were obtained with carrageenan 5g/L,tryptone 5g/L,NaCl 20g/L,CaCl_2 0.2g/L,NaH_2PO_4 · 2H_2O 1.3g/L,Na_2HPO_4 · 12H_2O 3.82g/L,tempreture 18℃,initial pH 6.5,inculation volume 2%,liquid volume 70 mL.Meanwhile,the three mediums were reduced comparing with the basal mediums.The biomass reached 1.4,which was 80.4%over initial biomass(0.776).Carrageenase activity reached 40.8 U/mL,and enzyme activity was 52.2%higher than initial carrageenase activity(26.8 U/mL).Then,the experiment of culture conditions in 5 L bioreactor was carried out.The results showed that high rotation speed can promote the rate of producing enzyme and short the ferment time,280 rpm was the optimal rotation speed,and that additional medium and control pH had not an obvious effect on improving enzyme production.Finally,the pilot enlarge experiment of 20 L,75 L and 200 L were conducted based on the ferment results of shaking flask and 5 L bioreactor.The maximum carrageenase activitives of 20 L,75 L and 200 L bioreactor reached 33.9 U/mL,34.7 U/mL and36.1 U/mL,respectively.And the maximum enzyme activity of 200 L bioreactor was 88.5%of the maximum enzyme activity of shaking flask,and 85.7%of the enzyme activity of 5 L bioreactor.
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