芍药苷防治小鼠急性肝损伤的机制研究
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- 英文论文题名：Study on the mechanism of the protective effects of Paeoniflorin on the mice with acute liver injury
- 论文作者：费立博
- 年：2016
- 论文关键词：芍药苷 ; 急性肝损伤 ; SphK1/S1P信号通路
- 英文论文关键词：Paeoniflorin ; acute liver injury ; SphK1/S1P signal pathway
- 会议召开时间：2016-06-17
- 会议录名称：2016中国中毒救治首都论坛——暨第八届全国中毒及危重症救治学术会议论文集
- 语种：中文
- 分类号：R285.5
- 学会代码：ZGDV
- 会议名称：2016中国中毒救治首都论坛——暨第八届全国中毒及危重症救治学术会议
- 会议地点：中国北京
- 主办单位：中国毒理学会中毒与救治专业委员会
- 学会名称：中国毒理学会
- 页数：4
- 文件大小：844k
- 原文格式：O
- 会议级别：全国
摘要
目的:观察芍药苷(PAE)对四氯化碳(CCl_4)诱导的小鼠急性肝损伤(ALI)的影响及其作用机制。方法:用腹腔注射CCl_4致小鼠急性肝损伤模型,测定不同剂量的PAE对肝损伤血清丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)活性、肝中炎症和脂肪代谢障碍的影响,同时对肝组织进行病理学检查。结果:PAE具有剂量依赖性地降低CCl_4致小鼠肝损伤血清ALT、AST、白介素1β(IL-1β)、IL-6、肿瘤坏死因子-α(TNF-α)、甘油三酯(TG)、总胆固醇(TC)和极低密度脂蛋白(VLDL)值升高,下调肝组织匀浆中卜磷酸鞘氨醇(S1P)的含量,抑制肝脏鞘氨醇激酶-1(SphK1)和肝脏核蛋白中核转录因子-kappaB(NF-κB)的蛋白表达水平,并减轻CCl_4对肝脏细胞的病理损伤。结论:PAE对CCl_4诱导的ALI小鼠的保护作用机制可能与抑制SphK1/S1P信号通路表达有关。
[Abstrac]Objective:To investigate the protective effects of Paeoniflorin(PAE) on the mice with acute liver injury(ALI) induced by CC1_4.Methods:The ALI model was prepared by CCU injected intraperitoneally.Then,the therapeutic effects of PAE on the model were evaluated by the activity determination of serum alanine aminotransferase(ALT) and aspartate aminotransferase(AST)and hepatic inflammation and lipid metabolism disorder,and the changes of hepatic pathohistology following the PAE treatment.Result:Following the treatment,PAE reduced the elevated ALT and AST activities and interleukin-1β(IL-1β),IL-6,tumor necrosis factor-a(TNF-α),triglyceride(TG),total cholesterol(TC) and very low density lipoprotein(VLDL) content induced by CC1_4 significantly,and downregulated the sphingosine-1-phosphate levels in liver homogenates,and inhibited hepatic sphingosine kinase 1(SphK1) and nucleoprotein-nuclear transcription factor-kappaB(NF-kB) protein expression.Furthermore,the pathological changes were also significantly improved in the mice of PAE treating group.Conclusion:The protective effects of PAE on the mice with ALI induced by CCL may be related to its inhibition of SphK 1/S1 P signal pathway.
[Abstrac]Objective:To investigate the protective effects of Paeoniflorin(PAE) on the mice with acute liver injury(ALI) induced by CC1_4.Methods:The ALI model was prepared by CCU injected intraperitoneally.Then,the therapeutic effects of PAE on the model were evaluated by the activity determination of serum alanine aminotransferase(ALT) and aspartate aminotransferase(AST)and hepatic inflammation and lipid metabolism disorder,and the changes of hepatic pathohistology following the PAE treatment.Result:Following the treatment,PAE reduced the elevated ALT and AST activities and interleukin-1β(IL-1β),IL-6,tumor necrosis factor-a(TNF-α),triglyceride(TG),total cholesterol(TC) and very low density lipoprotein(VLDL) content induced by CC1_4 significantly,and downregulated the sphingosine-1-phosphate levels in liver homogenates,and inhibited hepatic sphingosine kinase 1(SphK1) and nucleoprotein-nuclear transcription factor-kappaB(NF-kB) protein expression.Furthermore,the pathological changes were also significantly improved in the mice of PAE treating group.Conclusion:The protective effects of PAE on the mice with ALI induced by CCL may be related to its inhibition of SphK 1/S1 P signal pathway.
引文