东北梅花鹿遗传多样性及系统进化分析
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- 英文论文题名：Analysis of genetic diversity and phyletic evolution of cervus nippon horutrum
- 论文作者：李欢霞 ; 邢秀梅 ; 杨福合 ; 刘华淼 ; 赵东 ; 邵元臣 ; 杨颖
- 英文论文作者：Li huan-xia ; Sing xiu-mei ; Yang fu-he ; Liu hua-miao ; Zhao dong ; Shao Yuan-chen ; Yang-ying ; Institute of Special Animal and Plant Sciences of CAAS ; Jiangsu University Sicence and Technology
- 年：2016
- 作者机构：中国农业科学院特产研究所;江苏科技大学蚕业研究所;
- 论文关键词：梅花鹿 ; 线粒体基因组 ; 遗传多样性 ; 系统进化
- 英文论文关键词：cervus nippon ; mitochondrial genome ; genetic diversity ; phyletic evolution
- 会议召开时间：2016-06-01
- 会议录名称：“十二五” 鹿科学研究进展
- 语种：中文
- 分类号：S825
- 学会代码：ZXMY
- 学会名称：中国畜牧业协会
- 页数：8
- 文件大小：413k
- 原文格式：O
摘要
采用PCR直接测序法和克隆测序法,测定了东北梅花鹿4个品种共12个个体的线粒体基因组全序列,四个品种梅花鹿全长均为164 35bp,序列分析结果表明兴凯湖梅花鹿变异位点最多,然后依次为四平梅花鹿、东丰梅花鹿、敖东梅花鹿。四种梅花鹿的颠换对均小于转换对,四平梅花鹿的转换颠换率最大(R=15.0),其次为东丰梅花鹿(R=10.0)。兴凯湖梅花鹿与其他梅花鹿间的核苷酸距离较大,其中与东丰梅花鹿的核苷酸距离整体最大,梅花鹿的平均遗传距离为0.03。兴凯湖梅花鹿具有较高的核苷酸多样性(π=0.003 69),兴凯湖梅花鹿和东丰梅花鹿核苷酸间的歧异度Dxy最大(Dxy=0.004 82),系统进化关系为兴凯湖梅花鹿自成一单系,其他三个品种梅花鹿相互之间互有交叉。
Abstrat:We sequenced the complete mitochondrial genome of cervus nippon horutorum by PCR and Cloning.12 indiciduals from 4 populations of cervus nippon horutorum were included in this study.The complete mitochondrial genome length was 16435 base pairs.On the analysis:Xingkaihu breed had the highest variation sites,then from more to less was Siping breed,Dongfeng breed,Andong breed.The tansversion pairs of 4 populations were less than the transform pairs.Siping breed had the highest tansversion- transform rate(R = 15.0),then Dongfeng breed had the higher tansversion- transform rate(R = 10.0).Xingkaihu breed had the highest nucleotide distance,the nucleotide distance and the nucleotide divergence between xingkaihu breed and dongfengd breed were the greatest.The phylogenetie analysis displayed Xingkaihu breed was a separate group and other cervus nippon were cross mutually.
Abstrat:We sequenced the complete mitochondrial genome of cervus nippon horutorum by PCR and Cloning.12 indiciduals from 4 populations of cervus nippon horutorum were included in this study.The complete mitochondrial genome length was 16435 base pairs.On the analysis:Xingkaihu breed had the highest variation sites,then from more to less was Siping breed,Dongfeng breed,Andong breed.The tansversion pairs of 4 populations were less than the transform pairs.Siping breed had the highest tansversion- transform rate(R = 15.0),then Dongfeng breed had the higher tansversion- transform rate(R = 10.0).Xingkaihu breed had the highest nucleotide distance,the nucleotide distance and the nucleotide divergence between xingkaihu breed and dongfengd breed were the greatest.The phylogenetie analysis displayed Xingkaihu breed was a separate group and other cervus nippon were cross mutually.
引文
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[15]王忠武,马生良,李海,等.兴凯湖梅花鹿品种选育研究[J].经济动物学报,2004,8(1):1-6.
[2]Ohtaishi N.Preliminary memorandum of classification distribution and geographic variation on sika deer[J].Mamm.Sci,1986,53:13-17.
[3]Ohtaishi,N.and Y.T.Gao.A review of the distribution of all species of deer(T ragulidae,M oshidae and Cervidae)in China[J].Mammal,1990,Rev 20:125-144.
[4]郭延蜀,郑慧珍.中国梅花鹿地理分布的变迁[J].四川师范学院学报(自然科学版),1992,13(1):1-9.
[5]郭延蜀.中国梅花鹿地史分布、种和亚种的划分及演化历史[J].兽类学报,2000,20(3):1-12.
[6]邵伟庚.东北地区人工驯养梅花鹿线粒体控制区遗传特质分析[D].哈尔滨:东北林业大学.2004.
[7]程世鹏,刘彦.我国养鹿业的现状和发展方向[J].草食家畜,2006,4(133):1-3.
[8]吴华,胡杰,方盛国,等.中国圈养梅花鹿的遗传多样性和遗传结构[J].动物学杂志,2006,41(4):41-47.
[9]Sambrook J and David WR.Molecular Cloning:A Laboratory Manual(3rd edn)[M].New York;Cold Spring Harbor Laboratory Press,2001.
[10]Kumar S,K.Tamura,I.Jakobsen and M.Nei 2001 MEGA;molecular evolutionary genetic analysis,ver 2.0[J].Bioinf or matics,2001,17(12):1244-1245.
[11]Rozas.J and R.Rozas.DnaSP version 3:an integrated program for molecular population genetics and molecular evolution analysis[J].Bioinf or matics,1999,15:174-175.
[12]LIU Hai,YANG Guang and WEI Fu-Wen.Sequence variability of the mitochondrial DNA control region and population genetic structure of sika deers(Cervus nippon)in China[J].Acta Zoologica Sinica,2003,49(1):53-60.
[13]Hua Wu,Qiu-Hong Wan and Sheng-Guo Fang.Two genetically distinct units of the Chinese sika deer(cervus nippon):analyses of mitochondrial DNA variation[J].Biological Conservation,2004,119:183-190.
[14]LuXiaoping,Wei Fuwen and Li Ming.Genetic diversity among Chinese sika(Cervus Nippon)populations and relationships between Chinese and Janpanese sika deer Chinese[J].Science Bullentin,2006,51(3):433-440.
[15]王忠武,马生良,李海,等.兴凯湖梅花鹿品种选育研究[J].经济动物学报,2004,8(1):1-6.