大刺鳅微卫星标记开发及野生群体遗传多样性分析
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- 英文论文题名：Development of microsatellite markers and genetic diversity analysis in Mastacembelus armatus
- 论文作者：林婷婷 ; 房祖业 ; 舒琥 ; 李强 ; 江小璐 ; 吴咏诗 ; 陈晓东 ; 谭洁瑢 ; 张玮恩
- 英文论文作者：Tingting Lin ; Zuye Fang ; Hu Shu ; Qiang Li ; Xiaolu Jiang ; Yongshi Wu ; Xiaodong Chen ; Jierong Tan ; Weien Zhang ; School of Life Science ; Guangzhou University
- 年：2016
- 作者机构：广州大学生命科学学院;
- 论文关键词：大刺鳅 ; 微卫星位点 ; 遗传多样性
- 英文论文关键词：Mastacembelus armatus ; microsatellite markers ; genetic diversity
- 会议召开时间：2016-11-02
- 会议录名称：2016年中国水产学会学术年会论文摘要集
- 语种：中文
- 分类号：S917.4
- 学会代码：OGSB
- 会议名称：2016年中国水产学会学术年会
- 会议地点：中国四川成都
- 主办单位：中国水产学会、四川省水产学会
- 学会名称：中国水产学会
- 页数：1
- 文件大小：563k
- 原文格式：D
- 会议级别：全国
摘要
大刺鳅(Mastacembelus armatus)隶属合鳃目(Symbranchiformes)、刺鳅科(Mastacembelidae)、刺鳅属(Mastacembelus),是一种分布在华南地区的小型经济鱼类。本研究利用10对微卫星引物对不同地区大刺鳅野生群体的遗传多样性进行研究,结果显示,10个微卫星位点在283个大刺鳅个体中共检测出183个等位基因,平均等位基因数(NA)为2.1~11.2,观测杂合度(Ho)为0~0.933,期望杂合度(He)为0~0.946。多态信息含量(PIC)为0.038~0.922。Hardy-Weinberg平衡检验结果显示有若干个位点偏离平衡(P<0.05)。连锁不平衡检测表明各位点间无连锁不平衡现象。分子变异分析(AMOVA)表明,遗传变异来源主要在于个体间(46.344%)。遗传分化系数Fst为0.137~0.646,说明群体间存在极显著的遗传分化(FST>0.25)。Structure分析结果显示大刺鳅群体分为五个自由交配群。基于遗传距离构建的UPGMA聚类结果显示10个群体分为三支,西双版纳群体与其他群体距离最远。本研究旨在为大刺鳅的种质资源保护和人工繁殖提供理论依据。
We used 10 microsatellite markers and represented a population genetic analysis of 10 M. armatus wild populations collected from south China. Totally 183 alleles were obtained from the 10 microsatellite loci in 10 M. armatus populations. The observed and expected heterozygosity were ranged from 0 to 0.933 and from 0 to 0.946, respectively. Mean allele numbers at the 10 loci ranged from 2.1(Xishuangbanna) to 11.2(Lechang). The polymorphism information content(PIC)ranged from 0.038 to 0.922. Deviations from Hardy-Weinberg equilibrium were detected in some loci and and no significant linkage disequilibrium was observed between any two loci after the Bonferroni correction. The Fst values ranged from 0.137 to 0.646 which estimated in AMOVA indicated significant genetic differentiation among populations, the genetic variation mainly from individuals within populations(46.344%). Structure analysis showed that the 10 populations were grouped into five clusters. The UPGMA tree showed that the 10 populations clustered into three groups, Xishuangbanna population showed clear divergence to the other 9 populations.
We used 10 microsatellite markers and represented a population genetic analysis of 10 M. armatus wild populations collected from south China. Totally 183 alleles were obtained from the 10 microsatellite loci in 10 M. armatus populations. The observed and expected heterozygosity were ranged from 0 to 0.933 and from 0 to 0.946, respectively. Mean allele numbers at the 10 loci ranged from 2.1(Xishuangbanna) to 11.2(Lechang). The polymorphism information content(PIC)ranged from 0.038 to 0.922. Deviations from Hardy-Weinberg equilibrium were detected in some loci and and no significant linkage disequilibrium was observed between any two loci after the Bonferroni correction. The Fst values ranged from 0.137 to 0.646 which estimated in AMOVA indicated significant genetic differentiation among populations, the genetic variation mainly from individuals within populations(46.344%). Structure analysis showed that the 10 populations were grouped into five clusters. The UPGMA tree showed that the 10 populations clustered into three groups, Xishuangbanna population showed clear divergence to the other 9 populations.
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