高效液相色谱法测定饲料中艾司唑仑的含量
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- 英文论文题名：Determination of Estazolam in feeds by High Performance Liquid Chromatography
- 论文作者：方秋华 ; 肖田安 ; 伍宏凯 ; 胡苑玲
- 英文论文作者：Fang Qiuhua ; Xiao Tianan ; Wu Hongkai ; Hu Yuanling ; Guangdong Institute for Veterinary Drugs and Feedstuffs Control
- 年：2016
- 作者机构：广东省兽药饲料质量检验所;
- 论文关键词：艾司唑仑 ; 饲料 ; 高效液相色谱
- 英文论文关键词：Estazolam ; feeds ; high performance liquid chromatography(HPLC)
- 会议召开时间：2016-06-14
- 会议录名称：第25届广东省科技进步活动月畜牧兽医学术与科技创新发展大会论文集
- 语种：中文
- 分类号：S816.17
- 学会代码：GDKL
- 会议名称：第25届广东省科技进步活动月畜牧兽医学术与科技创新发展大会
- 会议地点：中国广东广州
- 主办单位：广东省畜牧兽医学会
- 学会名称：广东省科学技术协会科技交流部
- 页数：5
- 文件大小：477k
- 原文格式：O
- 会议级别：地方
摘要
本研究建立了高效液相色谱法测定预混合饲料、配合饲料和浓缩饲料中艾司唑仑含量的检测方法。试样用正己烷-二氯甲烷(1:1,V/V)提取,经MCX固相萃取柱进一步净化,采用反相高效液相色谱-紫外检测法进行定量分析。饲料中艾司唑仑的检测限为0.1 mg/kg,定量限为0.25 mg/kg。在饲料中添加0.25,0.5,2.5mg/kg水平的药物回收试验中,平均回收率均在82.8%~105.9%之间,相对标准偏差为1.62%~8.40%。该方法灵敏、准确,适用于饲料中艾司唑仑含量的分析检测。
A confirmatory method has been developed and validated for the determination of estazolam(EZL)in premix,compound feed and concentrate feed using high performance liquid chromatography(HPLC).The analyte was extracted from feeds using hexane-dichloromethane(1:1,V/V),and then the sample extracts were purified by MCX solid-phase extraction.The analyte was analyzed by HPLC-UV.The limit of detection was 0.1mg/kg for EZL,with quantification limit of 0.25 mg/kg.Recoveries were in the range 82.8%to 105.9%from feeds fortified at the 0.25,0.5,2.5 mg/kg levels,and the mean intra-laboratory reproducibility ranged from 1.62%to 8.40%(%R.S.D).The developed method is sensitive and specific for the detection of estazolam in feeds.
A confirmatory method has been developed and validated for the determination of estazolam(EZL)in premix,compound feed and concentrate feed using high performance liquid chromatography(HPLC).The analyte was extracted from feeds using hexane-dichloromethane(1:1,V/V),and then the sample extracts were purified by MCX solid-phase extraction.The analyte was analyzed by HPLC-UV.The limit of detection was 0.1mg/kg for EZL,with quantification limit of 0.25 mg/kg.Recoveries were in the range 82.8%to 105.9%from feeds fortified at the 0.25,0.5,2.5 mg/kg levels,and the mean intra-laboratory reproducibility ranged from 1.62%to 8.40%(%R.S.D).The developed method is sensitive and specific for the detection of estazolam in feeds.
引文
本文发表于《中国兽药杂志》2015年第4期P46-50。