牡丹、芍药花粉活力与柱头可授性研究
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- 英文论文题名：Pollen Viability and Stigma Receptivity of Paeonia ostii and Paeonia lactiflora
- 论文作者：贺丹 ; 解梦珺 ; 吕博雅 ; 王政 ; 刘艺平 ; 何松林
- 英文论文作者：HE Dan ; XIE Meng-jun ; LU Bo-ya ; WANG Zheng ; LIU Yi-ping ; HE Song-lin ; College of Forestry ; Henan Agricultural University
- 年：2016
- 作者机构：河南农业大学林学院;
- 论文关键词：牡丹 ; 芍药 ; 花粉活力 ; 柱头可授性
- 英文论文关键词：Paeonia ostii ; Paeonia lactiflora ; Viability ; Stigma receptivity
- 会议召开时间：2016-07-19
- 会议录名称：中国观赏园艺研究进展2016
- 语种：中文
- 分类号：S685.11;S682.12
- 学会代码：EGYP
- 会议名称：2016年中国观赏园艺学术研讨会
- 会议地点：中国湖南长沙
- 主办单位：中国园艺学会观赏园艺专业委员会、国家花卉工程技术研究中心
- 学会名称：中国园艺学会
- 页数：5
- 文件大小：1983k
- 原文格式：O
- 会议级别：全国
摘要
采用花粉离体萌发法研究牡丹品种‘凤丹白'、‘洛阳红',芍药品种‘粉玉奴'花粉萌发适宜Ca~(2+)浓度;电子显微镜观察花粉、柱头形态特征;联苯胺-过氧化氢法检测‘粉玉奴'柱头可授性。结果表明:①3个品种中,‘凤丹白'的穿孔频率最高,花粉畸形率最低;‘粉玉奴'的穿孔频率最低,花粉畸形率最高;②不同Ca~(2+)浓度条件下,‘凤丹白'在60mg·L~(-1)CaCl_2中花粉管伸长量最大;‘洛阳红'在40mg·L~(-1)CaCl_2浓度条件下花粉管伸长量最大;③‘粉玉奴'柱头在开花后1~6d均有可授性,其中,花后第3d柱头可授性最强。
The pollen germination in vitro was used to study the conditions suitable for Ca * concentration of pollen germination of peony varieties' Feng Dan' and ' Luo yang hong' and ' Fen yu nu';The Pollen and stigma morphology observed by microscope;The stigma receptivity was tested with benzidine-H_20_2 method.The results showed that:①The Perforated frequency of Feng Dan' was highest and the Pollen deformity rate was lowest;The Perforated frequency of ' Fen yu nu' was lowest and the Pollen deformity rate was highest;②The Pollen tube elongation of ' Feng Dan' was maximum that storage in 60 mg · L~(-1)CaCl_2 under the condition of different concentrations of Ca~(2+);③The Pollen tube elongation of ' Luo yang hong' was maximum that storage in 60 mg · L~(-1) CaCl_2 under the condition of different concentrations of Ca~(2+);The stigma of ' Fen yu nu' has to teach after flowering 1-6 days and the highest of stigma receptivity on the third day.
The pollen germination in vitro was used to study the conditions suitable for Ca * concentration of pollen germination of peony varieties' Feng Dan' and ' Luo yang hong' and ' Fen yu nu';The Pollen and stigma morphology observed by microscope;The stigma receptivity was tested with benzidine-H_20_2 method.The results showed that:①The Perforated frequency of Feng Dan' was highest and the Pollen deformity rate was lowest;The Perforated frequency of ' Fen yu nu' was lowest and the Pollen deformity rate was highest;②The Pollen tube elongation of ' Feng Dan' was maximum that storage in 60 mg · L~(-1)CaCl_2 under the condition of different concentrations of Ca~(2+);③The Pollen tube elongation of ' Luo yang hong' was maximum that storage in 60 mg · L~(-1) CaCl_2 under the condition of different concentrations of Ca~(2+);The stigma of ' Fen yu nu' has to teach after flowering 1-6 days and the highest of stigma receptivity on the third day.
引文
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8.张栋.2008.牡丹远缘杂交及部分杂交后代的AFLP分子标记鉴定[D].北京:北京林业大学.
9.荆丹丹,刘政安,李新旭,等.2011.牡丹远缘杂种‘和谐'育性的初步研究[J].林业科学,47(10):59-62.
10.郝青,刘政安,舒庆艳,等.2008.中国首例芍药牡丹远缘杂交种的发现及鉴定[J].园艺学报,35(6):853-858.
11.姬慧娟,尹林克,严成,等.2009.多枝柽柳的开花动态及花粉活力和柱头可授性研究[J].西北农林科技大学学报:自然科学版,37(5):114-118.
2.成仿云.1997.美国牡丹芍药协会与美国牡丹芍药的发展[J].西北师范大学学报(自然科学版),33(1):110-11.
3.郭绍霞,郑国生.2009.芍药切花瓶插期生理生化的变化[J].华北农学报,24(增刊):195-198.
4.孟凡聪,刘燕.2005.芍药花期调控研究进展[J].华北农学报,20(专辑):148-151.
5.肖佳佳.2010.芍药属杂交亲和性及杂种败育研究[D].北京:北京林业大学.
6.何桂梅.2006.牡丹远缘杂交育种及其胚培养与体细胞胚发生的研究[D].北京:北京林业大学.
7.侯甲男.2013.牡丹芍药组间远缘杂交及‘凤丹'多倍体诱导初探[D].郑州:河南农业大学.
8.张栋.2008.牡丹远缘杂交及部分杂交后代的AFLP分子标记鉴定[D].北京:北京林业大学.
9.荆丹丹,刘政安,李新旭,等.2011.牡丹远缘杂种‘和谐'育性的初步研究[J].林业科学,47(10):59-62.
10.郝青,刘政安,舒庆艳,等.2008.中国首例芍药牡丹远缘杂交种的发现及鉴定[J].园艺学报,35(6):853-858.
11.姬慧娟,尹林克,严成,等.2009.多枝柽柳的开花动态及花粉活力和柱头可授性研究[J].西北农林科技大学学报:自然科学版,37(5):114-118.