新型苯并噻唑衍生物作为pH荧光探针应用于细胞成像
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- 英文论文题名：A Novel Fluorescent pH Probe Based on benzothiazole Derivative for Intracellular Imaging
- 论文作者：樊丽 ; 南明 ; 董文娟 ; 林博 ; 双少敏 ; 董川
- 英文论文作者：Li Fan ; Ming Nan ; Wenjuan Dong ; Bo Lin ; Shaomin Shuang ; Chuan Dong ; Institute of Environmental Science ; Shanxi University
- 年：2016
- 作者机构：山西大学环境科学研究所;
- 论文关键词：噻唑衍生物 ; pH荧光探针 ; 细胞成像
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第二十八分会：化学生物学
- 语种：中文
- 分类号：O657.3
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第二十八分会 ; 化学生物学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：215k
- 原文格式：D
- 会议级别：全国
摘要
细胞内pH对于调控许多细胞的生理过程发挥重要的作用~([1]),pH值异常往往导致细胞功能紊乱~([2])。因此,对细胞内pH变化进行灵敏检测,有利于从分子水平上研究细胞的生理病理过程。本文利用乙烯基桥连苯并噻唑(电子给体,D)和喹啉醛(电子受体,A)设计合成了基于分子内电荷转移(ICT)效应的pH荧光探针(BTVQ)。以317 nm为激发波长,BTVQ的最大荧光发射位于428 nm(pH=7),随着pH由7.0降低到2.2,该发射峰逐渐减弱(Fig.1a)。通过激光共聚焦荧光成像技术,BTVQ成功实现人子宫颈鳞癌Siha细胞中pH变化的高灵敏响应(Fig.1b),可作为新型pH探针应用活细胞内酸性环境pH变化的检测。
Intracellular p H play an important role for regulating many cellular processes~([1]). The monitoring of p H changes in living cells, is critical for studying cellular functions and further understanding physiological and pathological processes. A novel p H fluorescent probes(BTVQ) based on intramolecular charge transfer(ICT) process was synthesized via the ethylene bridging of benzothiazole(D) and 2-quinolinecarbaldehyde(A). BTVQ exhibited fluorescent emission at 428 nm with an excitation wavelength of 318 nm(p H=7). Upon decreasing the p H from 7.0 to 2.2, the fluorescence intensity reduces gradually(Fig. 1a). The confocal fluorescent microscopic images of the highly sensitive p H response in Siha cells were achieved successfully(Fig. 1b), suggesting that BTVQ could be used as a novel p H probe for effectively detecting pH changes in acidic organelles.
Intracellular p H play an important role for regulating many cellular processes~([1]). The monitoring of p H changes in living cells, is critical for studying cellular functions and further understanding physiological and pathological processes. A novel p H fluorescent probes(BTVQ) based on intramolecular charge transfer(ICT) process was synthesized via the ethylene bridging of benzothiazole(D) and 2-quinolinecarbaldehyde(A). BTVQ exhibited fluorescent emission at 428 nm with an excitation wavelength of 318 nm(p H=7). Upon decreasing the p H from 7.0 to 2.2, the fluorescence intensity reduces gradually(Fig. 1a). The confocal fluorescent microscopic images of the highly sensitive p H response in Siha cells were achieved successfully(Fig. 1b), suggesting that BTVQ could be used as a novel p H probe for effectively detecting pH changes in acidic organelles.
引文
[1]Yang,M.;Song,Y.;Zhang,M.;Lin,X.;Hao,Z.;Liang,Y.;Zhang,M.;Chen,P.Angew.Chem.Int.Ed.2012,51:7674.
[2]Han,J.;Burgess,K.Chem.Rev.2010,110,2709.
[2]Han,J.;Burgess,K.Chem.Rev.2010,110,2709.