一株类芽孢杆菌(Paenibacillus sp.NJ-1)对苯系物的降解特性研究
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- 英文论文题名：Study on BTEX-degradation characteristics of a Strain NJ-1(Paenibacillus sp.)
- 论文作者：宾丽英 ; 李妙春 ; 李冠桂 ; 张雯翔 ; 汤兵
- 英文论文作者：Liying Bin ; Miaochun Li ; Guangui Li ; Wenxiang Zhang ; Bing Tang ; School of Environmental Science and Engineering ; Guangdong University of Technology ; Institute of Environmental Health and Pollution Control ; Guangdong University of Technology
- 年：2016
- 作者机构：广东工业大学环境科学与工程学院;广东工业大学环境健康与污染控制研究院;
- 论文关键词：类芽孢杆菌 ; NJ-1 ; 苯系物降解 ; 16S rDNA ; 甲苯单加氧酶
- 英文论文关键词：Paenibacillus sp. ; NJ-1 ; BTEX-degrading ; 16S rDNA ; Toluene mono-oxygenase
- 会议召开时间：2016-12-14
- 会议录名称：2016全国水环境污染控制与生态修复技术高级研讨会论文集
- 语种：中文
- 分类号：X172
- 学会代码：HJKP
- 会议名称：2016全国水环境污染控制与生态修复技术高级研讨会
- 会议地点：中国云南腾冲
- 主办单位：中国环境科学学会、中国环境科学研究院
- 学会名称：中国环境科学学会
- 页数：5
- 文件大小：942k
- 原文格式：D
- 会议级别：全国
摘要
本课题分别以苯、甲苯、二甲苯、乙苯作为唯一碳源和能源从广州石化炼油厂的废水处理站活性污泥分离筛选出一株能在不同的苯系物中生长的菌株,命名为NJ-1,经生理生化和16S r DNA鉴定为类芽孢杆菌属。采用甲苯作为唯一碳源进一步研究其生长、降解特性和机制。研究结果表明NJ-1的最适培养温度为32℃,最适生长p H为7.5~8.5;在32℃、p H7.0,初始甲笨浓度为500mg/L条件下,48h甲苯降解率达到66.44%;最适条件下对甲苯最高耐受浓度为60%(v/v);通过设计苯双加氧酶和甲苯单加氧酶、甲苯双加氧酶引物进行PCR扩增分析得出NJ-1对苯系物的降解经甲苯单加氧途径。
To select efficient strains which were used to degrade benzene compounds,a benzene-degrading bacterium(strain NJ-1)was isolated and purified from activated sludge around Petrochemical refinery in Guangzhou, China. NJ-1 could take benzene,toluene,xylene,benzene compounds as sole carbon and energy resources. By 16 S r DNA sequence homology analysis, it was identified as Paenibacillus sp..The optional growth condition for NJ-1 are 32℃,inoculation 10%,shaking rotate 180 r/min,p H7.5~8.5. PCR analysis was performed by designing primers, the BTEX-degrading way of strain NJ-1 is toluene mono-oxygenase. The highest tolerated toluene concentration of NJ-1 is 60%(v/v).
To select efficient strains which were used to degrade benzene compounds,a benzene-degrading bacterium(strain NJ-1)was isolated and purified from activated sludge around Petrochemical refinery in Guangzhou, China. NJ-1 could take benzene,toluene,xylene,benzene compounds as sole carbon and energy resources. By 16 S r DNA sequence homology analysis, it was identified as Paenibacillus sp..The optional growth condition for NJ-1 are 32℃,inoculation 10%,shaking rotate 180 r/min,p H7.5~8.5. PCR analysis was performed by designing primers, the BTEX-degrading way of strain NJ-1 is toluene mono-oxygenase. The highest tolerated toluene concentration of NJ-1 is 60%(v/v).
引文
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2.Nagarajan,K.,Loh,K.C.Formulation of microbial cocktails for BTEX biodegradation.Biodegradation,2015,26:51-63
3.Picone,S.Transport and Biodegradation of Volatile Organic Compounds:Influence on Vapor Intrusion into Buildings.Ph D thesis.Wageningen University,The Netherlands,2012.
4.孙文静.BTEX降解菌的驯化及其好氧降解规律的研究[D].天津大学,2009.
5.Kamal Khodaei,Hamid Reza Nassery,Mahnaz Mazaheri Asadi.BTEX biodegradation in contaminated groundwater using a novel strain(Pseudomonas sp.BTEX-30).International Biodeterioration&Biodegradation,2017,116:234-242
6.杨卫兵,朱润晔等.1株BTEX降解新菌株的分离鉴定及其降解特性研究[J].环境科学,2010,03:821-827.
7.东秀珠,蔡妙英等.常见细菌系统鉴定手册[M].科学出版社,2001:62-66.
8.王亚菲,李慧,李小彬等.沈抚灌区广宿主石油烃代谢质粒的分离及鉴定[J].应用生态学报,2013,24(11):3289-3299.
9.王琳,邵宗泽.4株苯系物降解菌菌株的筛选鉴定、降解特性及其降解基因研究[J].微生物学报,2006,05:753-757.