人居和荒漠草原生态条件下小花棘豆叶片蛋白质表达谱分析
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- 英文论文题名：A comparative analysis of protein expression profile of Oxytropis glabra under adverse environment
- 论文作者：周攀 ; 吴道长 ; 王德军 ; 李国中 ; 尉亚辉
- 英文论文作者：Zhou Pan ; Wu Saochang ; Wang Sejun ; Li Guozhong ; Wei Yahui ; Key Laboratory of Resource Biology and Biotechnology in Western China ; Ministry of Education ; Shaanxi Provincial Key Laboratory of Biotechnology ; College of Life Science ; Northwest University ; Elisi Tengri Alxa Left League Animal Health Surveillance ; Alxa Left League Animal Health Supervision
- 年：2014
- 作者机构：西北大学生命科学院西部资源与现代生物技术省部共建教育部重点实验室/陕西省生物技术重点实验室;内蒙古阿拉善左旗腾格里额里斯动物卫生监督站;内蒙古阿拉善左旗动物卫生监督所;
- 论文关键词：人居生态条件 ; 荒漠生态条件 ; 叶片 ; 蛋白质 ; 表达谱
- 英文论文关键词：Living environment ; Desert grassland environment ; Blade ; Protein ; Expression profile
- 会议召开时间：2014-11-29
- 会议录名称：陕西省毒理学会2014年学术会议论文集
- 语种：中文
- 分类号：Q946
- 学会代码：XBST
- 会议名称：陕西省毒理学会2014年学术会议
- 会议地点：中国陕西西安
- 主办单位：陕西省毒理学会
- 学会名称：西北大学生态毒理研究所
- 页数：11
- 文件大小：977k
- 原文格式：O
- 会议级别：地方
摘要
选取公园(A地)和荒漠草原(B地)两种生境下的小花棘豆,对两地土壤指标分析,B地相对A地干旱、高盐、氮磷含量偏低。利用双向电泳技术,对比蛋白质差异表达谱,选取其中的差异蛋白点进行MADLI-TOF-MS鉴定,获得的PMF数据经Mascot软件在NCBI NR数据库上检索分析后确认蛋白种类。相对A地,B地小花棘豆光合作用相关调控酶类表达下调,而光合作用结构蛋白如叶绿素球蛋白表达上调。通过光合仪测量,B地小花棘豆光合速率下降,可能是因为光合调控酶表达下调影响了它的光合速率。而B地ATP合成相关蛋白的表达上调,说明干旱、高盐、氮磷含量偏低的环境下生长可能需要消耗更多能量。另外B地小花棘豆中发现一些跟胁迫相关蛋白出现上调或者特异性表达,这些蛋白的表达可能跟小花棘豆能够适应逆境有着密切的关系。
Two habitats of Oxytropis glabra were selected from park(place A) and desert steppe(place B),and we analyzed the soil index of the two places.Place B is drier and has high salinity,low nitrogen and phosphorus content than place A.The differences in protein expression profile of plants from the above two sites were compared using two-dimensional electrophoresis technology and the different protein spots were identified by MADLI-TOF-MS.The identities of the proteins were confirmed by Mascot software against the NCBI NR database.The regulating enzymes of photosynthesis of Oxytropis glabra in place B decreased compared to the sample in place A.But the structural proteins of photosynthesis such as chlorophyll and globulin were up-regulated.Photosynthetic rate of Oxytropis glabra decreased in place B measured by the photosynthetic apparatus,probably because of the reduced expression of photosynthetic regulation enzyme affecting its photosynthetic rate.Furthermore,the abundance of ATP synthesis-related proteins in place B increased,which indicated the samples in drought,high salinity,low nitrogen and phosphorus content of the growth environment need to consume more energy.In addition,it was found that some up-regulated proteins in Oxytropis glabra from place B were specific proteins associated with stress.The expression of these proteins may enable Oxytropis glabra to live in adverse environment.
Two habitats of Oxytropis glabra were selected from park(place A) and desert steppe(place B),and we analyzed the soil index of the two places.Place B is drier and has high salinity,low nitrogen and phosphorus content than place A.The differences in protein expression profile of plants from the above two sites were compared using two-dimensional electrophoresis technology and the different protein spots were identified by MADLI-TOF-MS.The identities of the proteins were confirmed by Mascot software against the NCBI NR database.The regulating enzymes of photosynthesis of Oxytropis glabra in place B decreased compared to the sample in place A.But the structural proteins of photosynthesis such as chlorophyll and globulin were up-regulated.Photosynthetic rate of Oxytropis glabra decreased in place B measured by the photosynthetic apparatus,probably because of the reduced expression of photosynthetic regulation enzyme affecting its photosynthetic rate.Furthermore,the abundance of ATP synthesis-related proteins in place B increased,which indicated the samples in drought,high salinity,low nitrogen and phosphorus content of the growth environment need to consume more energy.In addition,it was found that some up-regulated proteins in Oxytropis glabra from place B were specific proteins associated with stress.The expression of these proteins may enable Oxytropis glabra to live in adverse environment.
引文
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[2]史志诚.中国草地重要有毒植物[M].北京:中国农业出版社,1997:13-15,45-66Shi Zhicheng.Important poisonous plants in Chinese grassland[M].Beijing:Chinese Agriculture Press,1997:13-15,45-66.
[3]赵宝玉,童德文,葛鹏斌,等.我国西部草原疯草危害调查[J].中国草地学报,2003,25(4):65-68.Zhao Baoyu,Tong Dewen,Ge Pengbing,et al.Western China grassland locoweed hazard survey[J],Chinese Journal of Grassland,2003,25(4):65-68.
[4]程翠,王雨梅,赵萌莉,等.内生真菌对小花棘豆种子抗渗透胁迫能力的影响[J].中国草地学报,2009,31(5):84-89.Cheng Cui,Wang Yumei,Zhao Mengli,et al.Effects of Endophyte on Germination,Growth and Resistance to Osmotic Stress of Oxytropis glabra[J].Chinese Journal of Grassland,2009,31(5):84-89.
[5]樊泽峰,王建军,赵宝玉,等.内蒙古阿拉善盟草原疯草危害调查[J].中国草地学报,2006,28(2):56-59.FAN Zefeng,Wang Jianjun,Zhao Baoy u,Locoweed harm investigation in alasan league of Inner Mongolia[J].Chinese Journal of Grassland,2006,28(2):56-59.
[6]卢萍,赵萌莉,韩国栋,等.内蒙古疯草及其研究进展[J].中国草地学报,2006,28(1):63-68.Lu Ping,Zhao Mengli,Han Guodun,et al.The Locoweed in Inner Mongolia and Research Progress[J].Chinese Journal of Grassland,2006,28(1):63-68.
[7]樊鹏辉,郭斌,吴道长,等.中国草地有毒棘豆的危害防控及开发利用[J].中国草地学报,2012,34(6):101-106.Fan Penghui,Guo Bin,Wu Daochang,et al.The hazards,prevention and exploitation of poisonous oxytropis in grassland of China[J].Chinese Journal of Grassland,2012,34(6):101-106.
[8]Yoshimura K,Masuda A,Kuwano M,et al.Programmed proteome response for drought avoidance/tolerance in the root of a C3 xerophyte(wild watermelon)under water deficits[J].Plant and Cell Physiology,2008,49(2):226-241.
[9]Aghaei K,Ehsanpour A A,Shah A H,et al.Proteome analysis of soybean hypocotyl and root under salt stress[J].Amino Acids,2009,36(1):91-98.
[10]Jellouli N,Ben Jouira H,Skouri H,et al.Proteomic analysis of Tunisian grapevine cultivar Razegui under salt stress[J].Journal of Plant Physiology,2008,165(5):471-481.
[11]Jorge I,Navarro R M,Lenz C,et al.Variation in the holm oak leaf proteome at different plant developmental stages,between provenances and in response to drought stress[J].Proteomics,2006,6(1):207-214.
[12]劳家柽.土壤农化分析手册[M].北京:中国农业出版社,1988.12.Lao Jiacheng.Soil analysis manual[M].Bei Jing:China Agriculture Press.1988.12.
[13]Luo Haibo,Bao Yonghua,Jiang Juan,et al.Proteome changes of fresh-cut Zizania latifolia during refrigerated(1℃)storage[J].European Food Research and Technology.,2012,235(6):1011-1021.
[14]Hiroyuki Katayama,Takeshi Nagasu,Yoshiya Oda.Improvement of in-gel digestion protocol for peptide mass fingerprinting by matrix-assisted laser desorption/ionizati on time-of-flight mass spectrometry[J].Rapid Communications in Mass Spectrometry,2001,15(16):1416-1421.
[15]Hiroki Ashidaa,Antoine Danchinb,Akiho Yokotaa.Was photosynthetic RuBisCO recruited by acquisitive evolution from RuBisCO-like proteins involved in sulfur metabolism?[J].Research in microbiology,2005,156(5):611-618.
[16]Ghulam Muhammad Ali,Setsuko Komatsu.Proteomic analysis of rice leaf sheath during drought stress[J].Journal of Proteome Research,2006,5(2):396-403.
[17]陶佩琳.低磷胁迫下玉米叶片的光合特性及叶蛋白质组学研究[D].山东大学,2009Tao Peilin.Studies on photosynthetic characteristics and leaves proteomics maize inbred lines under low phosphorus stress[D].Shandong University.2009.
[18]Yu Shunwu,Tang Kexuan.MAP kinase cascades responding to environmental stress in plants[J].Acta Botanica Sinica-English Edition,2004,46(2):127-136.
[19]Richardson K E,Tolbert N E.Oxidation of glyoxylic acid to oxalic acid by glycolic acid oxidase[J].Journal of Biological Chemistry,1961,236(5):1280-1284.
[20]王炜军,李明启.菜心乙醇酸氧化酶二种催化功能的初步研究[J].热带亚热带植物学报,1999,7(2):177-180Wang Weijun,Li Mingqi.Preliminary study on two types of catalytic function of glycollate oxidase in the leaves of Brassica Parachinensis[J]Journal of Tropical and Subtropical Botany.1999,7(2):177-180.
[21]严小龙,张福锁.植物营养遗传学[M].北京:中国农业出版社,1997.113-116Yan Xiaolong,Zhang Fusuo.Genetics of plant nutrition[M].China Agriculture Press.1997.113-116
[22]沈文飚,黄丽琴,徐朗莱.植物抗坏血酸过氧化物酶[J].生命的化学,1997,17(5):112-115.Sheng Wenbiao,Huang liqin,Xu langlai.Ascorbic acid peroxidase of plants[J].Chemistry of Life,1997,17(5):112-115.