基于2-(2-丙炔胺基)-N-(8-喹啉基)乙酰胺荧光探针的合成及其在水中接力识别Zn~(2+),S~(2-)和PPi离子
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- 英文论文题名：A Fluorescence Probe for Relay Recognition of Zn~(2+), S~(2-) and PPi In Water Based on 2-(2-propynamino)-N-(quinolin-8-yl) Acetamide
- 论文作者：赵杰 ; 周雪 ; 郭宝峰 ; 钟克利
- 英文论文作者：Jie Zhao ; Xue Zhou ; Baofeng Guo ; Keli Zhong ; College of Chemistry and Chemical Engineering ; Bohai University.Food Safety Key Lab of Liaoning Province
- 年：2016
- 作者机构：渤海大学化学化工学院辽宁省食品安全重点实验室;
- 论文关键词：荧光探针 ; 喹啉衍生物 ; 锌离子 ; 硫离子 ; 焦磷酸根离子
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第二十七分会：光化学
- 语种：中文
- 分类号：O657.3
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第二十七分会 ; 光化学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：336k
- 原文格式：D
- 会议级别：全国
摘要
锌是生理上重要的过渡金属之一,而硫的毒性以及焦磷酸根在生理和代谢中的重要作用,使它们的检测非常重要。本文利用8-氨基喹啉通过两步反应,成功合成了一种具有三重功能的水溶性荧光探针L。在HEPES缓冲溶液(1%DMSO,10 m M,pH=7.4)中L可以荧光"关-开"识别Zn~(2+),所形成的配合物又能接力识别S~(2-)和P_2O_7~(4-)。实验表明S2-能脱除络合物中的Zn~(2+),而P_2O_7~(4-)是与Zn~(2+)发生了络合。该探针能成功检测出芽孢杆菌和金黄色葡萄球菌中的Zn~(2+),说明可以用于生物体中Zn~(2+)的检测。
Zinc, one of the most important transition metal of physiological importance, and sulfide, pyrophosphate(PPi) detection have also received considerable attention because of notorious toxicity of sulfide and critical roles of PPi in several bioenergetics and metabolic processes. Hence, we designed a water solubility fluorescent probe L with triple functions synthesized through two-step reaction. L detected Zn~(2+) with a fluorescent "OFF-ON" response in HEPES buffer solution(1% DMSO, 10 m M, pH=7.4). X-ray crystal structure of L–Zn~(2+) reveals binding stoichiometry is 1:1. The obtained L-Zn~(2+) complex can relay the detection of S~(2-) and P_2O_7~(4-). The experiment results showed that Zn~(2+) of the complex was removed by S~(2-), and was combined with P_2O_7~(4-). The Zn~(2+) within the Bacillus and Staphylococcus was detected, which declared the fluorescence probe L was applied in the biological system.
Zinc, one of the most important transition metal of physiological importance, and sulfide, pyrophosphate(PPi) detection have also received considerable attention because of notorious toxicity of sulfide and critical roles of PPi in several bioenergetics and metabolic processes. Hence, we designed a water solubility fluorescent probe L with triple functions synthesized through two-step reaction. L detected Zn~(2+) with a fluorescent "OFF-ON" response in HEPES buffer solution(1% DMSO, 10 m M, pH=7.4). X-ray crystal structure of L–Zn~(2+) reveals binding stoichiometry is 1:1. The obtained L-Zn~(2+) complex can relay the detection of S~(2-) and P_2O_7~(4-). The experiment results showed that Zn~(2+) of the complex was removed by S~(2-), and was combined with P_2O_7~(4-). The Zn~(2+) within the Bacillus and Staphylococcus was detected, which declared the fluorescence probe L was applied in the biological system.
引文
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[2]Tang L.,Zheng Z.,Huang Z.,et al.RSC Adv.,2015,5:10505–10511.
[2]Tang L.,Zheng Z.,Huang Z.,et al.RSC Adv.,2015,5:10505–10511.