肝癌特异性靶向黏附肽A54修饰的金纳米笼药物递送系统的制备及其体外释药特性的研究
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- 英文论文题名：Preparation of Hepatocarcinoma-binding Peptide A54-targeted AuNCs-based Delivery System and Its Release Properties in Vitro
- 论文作者：黄胜楠 ; 刘莹 ; 王卫萍 ; 李春明 ; 李焕杰 ; 胡玉荣
- 英文论文作者：HUANG Sheng-nan ; LIU Ying ; WANG Wei-ping ; LI Chun-ming ; LI Huan-jie ; HU Yu-rong ; School of Pharmaceutical Sciences ; Zhengzhou University ; Department of Pharmacy ; The First Affiliated Hospital of Xinxiang Medical University
- 年：2016
- 作者机构：郑州大学药学院;新乡医学院第一附属医院;
- 论文关键词：金纳米笼 ; 靶向黏附肽A54 ; 可控释放 ; 肝癌
- 英文论文关键词：gold nanocages(AuNCs) ; hepatocarcinoma-binding peptide A54 ; controlled-release ; hepatocellular carcinoma(HCC)
- 会议召开时间：2016-09-27
- 会议录名称：中国药学会第十三届青年药学科研成果交流会论文集
- 语种：中文
- 分类号：R943;R96
- 学会代码：YYWS
- 会议名称：中国药学会第十三届青年药学科研成果交流会
- 会议地点：中国江西南昌
- 主办单位：中国药学会
- 学会名称：中国药学会
- 页数：5
- 文件大小：1048k
- 原文格式：O
- 会议级别：全国
摘要
目的:制备肝癌特异性靶向黏附肽A54修饰的金纳米笼药物递送系统,考察其体外释药特性。方法:以具有中空结构的金纳米笼为载体,以酶敏感的透明质酸为控释的闸门,以肝癌特异性靶向黏附肽A54为靶头,制备肝癌特异性靶向黏附肽A54修饰的金纳米笼药物递送系统(DHTPAuNCs)。运用激光纳米粒度仪测定其粒径电位;透射电镜观察其形貌;紫外可见分光光度仪扫描其光谱变化;采用透析法考察其体外释药特性。结果:DHTPAuNCs的平均粒径为105.0±5.78 nm,电位为-12.50 mV,载药量为5.78%。DHTPAuNCs能够实现可控释放,透明质酸酶会触发药物释放,而低pH环境和近红外光照射可以加速药物释放。结论:成功制备了肝癌特异性靶向黏附肽A54修饰的金纳米笼药物递送系统,其载药量高,体外释药具有可控性。
Objective To prepare hepatocarcinoma-binding peptide A54-targeted AuNCs-based delivery system(DHTPAuNCs)and investigate its release properties in vitro.METHODS DHTPAuNCs was prepared with hollow & porous structure-based gold nanocages(AuNCs) as carrier material,enzyme-sensitive hyaluronic acid as gatekeeper and hepatocarcinoma-binding peptide A54 as target ligand.The mean particle size and Zeta potential was measured by laser particle size analyzer;the morphology of DHTPAuNCs was observed by transmission electron microscope;the optical properties were characterized using an UV-vis-NIR spectrometer;the in vitro release behavior was studied by dialysis.RESULTS The mean particle size was 105.0 ± 5.78 nm,the Zeta potential was-12.50 mV and the drug loading content was 5.78%.DHTPAuNCs were capable of triggering DOX release by intracellular enzyme Hyal and NIR laser or at acidic pH could accelerate the release profile.CONCLUSION DHTPAuNCs with high drug loading content was successfully prepared,and displayed controlled-release property in vitro.
Objective To prepare hepatocarcinoma-binding peptide A54-targeted AuNCs-based delivery system(DHTPAuNCs)and investigate its release properties in vitro.METHODS DHTPAuNCs was prepared with hollow & porous structure-based gold nanocages(AuNCs) as carrier material,enzyme-sensitive hyaluronic acid as gatekeeper and hepatocarcinoma-binding peptide A54 as target ligand.The mean particle size and Zeta potential was measured by laser particle size analyzer;the morphology of DHTPAuNCs was observed by transmission electron microscope;the optical properties were characterized using an UV-vis-NIR spectrometer;the in vitro release behavior was studied by dialysis.RESULTS The mean particle size was 105.0 ± 5.78 nm,the Zeta potential was-12.50 mV and the drug loading content was 5.78%.DHTPAuNCs were capable of triggering DOX release by intracellular enzyme Hyal and NIR laser or at acidic pH could accelerate the release profile.CONCLUSION DHTPAuNCs with high drug loading content was successfully prepared,and displayed controlled-release property in vitro.
引文
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[2]ZHENG M B.Application of degradable photosensitive nanoparticles in image tracing and cancer theranostics[D].Beijing:University of Chinese Academy of Sciences,2014.
[3]LIU Y.Enzyme/Laser-responsive Controlled Drug Release Systems Based on Mesoporous Silica Nanoparticles and Their Biological Evaluations[D].Chongqing:Chongqing University,2015.
[4]ZHONG H,XU H P,ZHANG HUI.Preparation and Application in Tumor Cells'Drug Delivery of Gold Nanocapsule.Chinese Journal of Analytical Chemistry(分析化学研究报告),2014,42(4):475-481.
[5]HAN X,CAO J Y Q,ZHOU J L,et al.pH-responsive Dual-drug Delivery System Based on Chitosan.ACTA POLYME RICA SINICA(高分子学报),2015,12:1471-1476.
[6]Chen J,Wiley B,Li ZY,et al.Gold Nanocages:Engineering Their Structure for Biomedical Applications.ADV MATER,2005,17(18):2255-2261.