双酚S对3T3-L1前脂肪细胞脂代谢及其作用机制研究
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- 英文论文题名：Study on the effect and mechanism of BPS on lipid metabolism in 3T3-L1 preadipocytes
- 论文作者：周兴华 ; 孙鑫娟 ; 牟妍 ; 祝莹 ; 周越 ; 伍娟 ; 董英
- 英文论文作者：Zhou Xinghua ; Sun Xinjuan ; Mu Yan ; Zhu Ying ; Zhou Yue ; Wu Juan ; Dong Ying ; School of Food and Biological Engineering ; Jiangsu University ; Yantai Entry-Exit Inspection And Quarantine
- 年：2016
- 作者机构：江苏大学食品与生物工程学院;烟台出入境检验检疫局;
- 论文关键词：双酚S ; 双酚A ; 3T3-L1前脂肪细胞 ; 脂代谢 ; 作用途径
- 英文论文关键词：bisphenol S ; bisphenol A ; 3T3-L1 preadipocytes ; lipid metabolism ; pathways
- 会议召开时间：2016-11-09
- 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
- 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
- 语种：中文
- 分类号：Q2-33
- 学会代码：ZGSP
- 会议名称：中国食品科学技术学会第十三届年会
- 会议地点：中国北京
- 主办单位：中国食品科学技术学会
- 学会名称：中国食品科学技术学会
- 页数：2
- 文件大小：114k
- 原文格式：O
- 会议级别：全国
摘要
本研究以3T3-L1前脂肪细胞为体外模型,探究低浓度双酚A(BPA)和双酚S(BPS)对3T3-L1前脂肪细胞分化及其脂代谢作用途径。试验采用CCK-8法检测细胞增殖能力、油红O染色法分析前脂肪细胞的分化程度;Realtime PCR法检测LFBE对脂代谢关键基因转录水平的调控作用。结果表明,低浓度两种双酚物质均能促进3T3-L1前脂肪细胞生长,但当其浓度超过100μmoL/L时,二者都能显著抑制细胞的增殖。BPA和BPS都可以诱导分化的3T3-Ll细胞内脂质的积累,其中BPA作用更为明显,10~(-15)mol/L BPA和10~(-13)BPSmoL/L作用下,细胞分化率分别165.69%和138.93%(p<0.01),并呈现非剂量依赖关系。BPA能增加脂肪细胞分化关键基因如PPARγ和aP2mRNA等的表达,而BPS影响不同甚至下调一些基因的表达,但是两种酚类物质均能下调GLUT4 mRNA的表达。推测BPS和BPA诱导前脂肪细胞分化的过程极其复杂,两者可能通过不同的作用途径来影响脂代谢。
The adipose cell line 3T3-L1 was used as the in vitro model to compare the effects of low doses of BPS and BPA,on on differentiation and lipid metabolism.3T3-L1 preadipocytes were used to research the effect on their proliferation through CCK- 8 assay and on the differentiation through oil red O staining,the mRNA expression levels of adipogenic genes were measured by real time-polymerase chain reaction(RT-PCR).The main research contents and conclusions are as follows:the low concentrations of bisphenols could promote the growth of 3T3-L1 cells,However,bisphenols at over 100 μmol/L had a greatly significant difference on inhibition of proliferation.Both BPA and BPS could induce the accumulation of lipid in the differentiated 3T3-L1 cells,and the role of BPA is more obvious,for example,the differentiation rates are 165.69%and 138.93%(p <0.01) treated with 10~(-15)mol/L BPA and 10~(13)mol/L BPS respectly,and it has no dose-response relationship.These effects could be linked to the modulation of PPARγ,aP2 and GLUT4 genes after exposure to BPA,whereas BPS seems has different effect in expression of these genes.The findings suggest that both BPA and BPS could be involved in lipid metabolism,but through two different metabolic pathways.
The adipose cell line 3T3-L1 was used as the in vitro model to compare the effects of low doses of BPS and BPA,on on differentiation and lipid metabolism.3T3-L1 preadipocytes were used to research the effect on their proliferation through CCK- 8 assay and on the differentiation through oil red O staining,the mRNA expression levels of adipogenic genes were measured by real time-polymerase chain reaction(RT-PCR).The main research contents and conclusions are as follows:the low concentrations of bisphenols could promote the growth of 3T3-L1 cells,However,bisphenols at over 100 μmol/L had a greatly significant difference on inhibition of proliferation.Both BPA and BPS could induce the accumulation of lipid in the differentiated 3T3-L1 cells,and the role of BPA is more obvious,for example,the differentiation rates are 165.69%and 138.93%(p <0.01) treated with 10~(-15)mol/L BPA and 10~(13)mol/L BPS respectly,and it has no dose-response relationship.These effects could be linked to the modulation of PPARγ,aP2 and GLUT4 genes after exposure to BPA,whereas BPS seems has different effect in expression of these genes.The findings suggest that both BPA and BPS could be involved in lipid metabolism,but through two different metabolic pathways.
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