摘要
本研究以3T3-L1前脂肪细胞为体外模型,探究低浓度双酚A(BPA)和双酚S(BPS)对3T3-L1前脂肪细胞分化及其脂代谢作用途径。试验采用CCK-8法检测细胞增殖能力、油红O染色法分析前脂肪细胞的分化程度;Realtime PCR法检测LFBE对脂代谢关键基因转录水平的调控作用。结果表明,低浓度两种双酚物质均能促进3T3-L1前脂肪细胞生长,但当其浓度超过100μmoL/L时,二者都能显著抑制细胞的增殖。BPA和BPS都可以诱导分化的3T3-Ll细胞内脂质的积累,其中BPA作用更为明显,10~(-15)mol/L BPA和10~(-13)BPSmoL/L作用下,细胞分化率分别165.69%和138.93%(p<0.01),并呈现非剂量依赖关系。BPA能增加脂肪细胞分化关键基因如PPARγ和aP2mRNA等的表达,而BPS影响不同甚至下调一些基因的表达,但是两种酚类物质均能下调GLUT4 mRNA的表达。推测BPS和BPA诱导前脂肪细胞分化的过程极其复杂,两者可能通过不同的作用途径来影响脂代谢。
The adipose cell line 3T3-L1 was used as the in vitro model to compare the effects of low doses of BPS and BPA,on on differentiation and lipid metabolism.3T3-L1 preadipocytes were used to research the effect on their proliferation through CCK- 8 assay and on the differentiation through oil red O staining,the mRNA expression levels of adipogenic genes were measured by real time-polymerase chain reaction(RT-PCR).The main research contents and conclusions are as follows:the low concentrations of bisphenols could promote the growth of 3T3-L1 cells,However,bisphenols at over 100 μmol/L had a greatly significant difference on inhibition of proliferation.Both BPA and BPS could induce the accumulation of lipid in the differentiated 3T3-L1 cells,and the role of BPA is more obvious,for example,the differentiation rates are 165.69%and 138.93%(p <0.01) treated with 10~(-15)mol/L BPA and 10~(13)mol/L BPS respectly,and it has no dose-response relationship.These effects could be linked to the modulation of PPARγ,aP2 and GLUT4 genes after exposure to BPA,whereas BPS seems has different effect in expression of these genes.The findings suggest that both BPA and BPS could be involved in lipid metabolism,but through two different metabolic pathways.
引文