酶催化反应调控纳米金用于可视化免疫分析
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- 英文论文题名：Enzyme-Mediated Modulation of Gold Nanoparticles for Plasmonic Immunoassays
- 论文作者：鲜于运雷 ; 蒋兴宇
- 英文论文作者：Yunlei Xianyu ; Xingyu Jiang ; National Center for Nanoscience and Technology
- 年：2016
- 作者机构：国家纳米科学中心;
- 论文关键词：碱性磷酸酶 ; 辣根过氧化物酶 ; 纳米金 ; 可视化读出 ; 免疫检测
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第三十八分会：纳米生物效应与纳米药物化学
- 语种：中文
- 分类号：TB383.1;R318
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第三十八分会 ; 纳米生物效应与纳米药物化学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：163k
- 原文格式：D
- 会议级别：全国
摘要
发展高灵敏可视化的免疫分析法对生物医学诊断具有重要意义,我们设计了酶催化的级联反应调控纳米金的表面等离子体共振信号用于可视化的免疫分析:(1)利用碱性磷酸酶催化的点击化学反应构建了可视化的免疫分析方法;(2)利用辣根过氧化物酶和碘离子催化的反应构建了可视化的免疫分析方法。和传统免疫分析方法相比,这种基于酶调控纳米金光学信号的方法不需要复杂的仪器设备,能够实现高灵敏和肉眼可视化的读出。
Current techniques for plasmonic immunoassay often require the introduction and additional conjugation of enzyme, thus cannot accommodate conventional immunoassay platforms. We propose a methodology for plasmonic immunoassay using alkaline phosphatase-triggered click chemistry between azide/alkyne functionalized gold nanoparticles as the readout. It well accommodates the current immunoassay platforms and allows naked-eye readout of immunoassays with enhanced sensitivity and stability, which holds great promise for applications in resource-constrained settings.
Current techniques for plasmonic immunoassay often require the introduction and additional conjugation of enzyme, thus cannot accommodate conventional immunoassay platforms. We propose a methodology for plasmonic immunoassay using alkaline phosphatase-triggered click chemistry between azide/alkyne functionalized gold nanoparticles as the readout. It well accommodates the current immunoassay platforms and allows naked-eye readout of immunoassays with enhanced sensitivity and stability, which holds great promise for applications in resource-constrained settings.
引文
[1]Xianyu,Y.;Jiang,X.ACS Nano 2014,8:12741.
[2]Xianyu,Y.;Jiang,X.Small 2014,10:4833.
[3]Xianyu,Y.;Jiang,X.Small 2015,11:5510.
[4]Xianyu,Y.;Jiang,X.Anal.Chem.2015,87:10688.
[2]Xianyu,Y.;Jiang,X.Small 2014,10:4833.
[3]Xianyu,Y.;Jiang,X.Small 2015,11:5510.
[4]Xianyu,Y.;Jiang,X.Anal.Chem.2015,87:10688.