不同食品接触材料表面副溶血性弧菌生物被膜比较蛋白质组学研究
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- 英文论文题名：Comparativeproteomic analysis of biofilm formed by Vibrio parahaemolyticus at various food contact surfaces
- 论文作者：宋雪迎 ; 赵爱静 ; 韩乔 ; 赵勇
- 英文论文作者：Song Xueying ; Zhao Aijing ; Han Qiao ; Zhao Yong ; College of Food Science and Technology ; Shanghai Ocean University ; Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation ; Laboratory of Quality & Safety Risk Assessment for Aquatic Product on Storage and Preservation(Shanghai) ; Ministry of Agriculture
- 年：2016
- 作者机构：上海海洋大学食品学院;上海水产品加工及贮藏工程技术研究中心;农业部水产品贮藏保鲜质量安全风险评估实验室;
- 论文关键词：副溶血性弧菌 ; 生物被膜 ; 蛋白质组学 ; 食品接触表面
- 英文论文关键词：vibrio parahaemolyticus ; biofilm ; proteomic ; food contact surfaces
- 会议召开时间：2016-11-09
- 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
- 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
- 语种：中文
- 分类号：TS201.6
- 学会代码：ZGSP
- 会议名称：中国食品科学技术学会第十三届年会
- 会议地点：中国北京
- 主办单位：中国食品科学技术学会
- 学会名称：中国食品科学技术学会
- 页数：2
- 文件大小：109k
- 原文格式：O
- 会议级别：全国
摘要
本研究采用结晶紫染色法和扫描电镜法分析了副溶血性弧菌(Vibrio parahaemolyticus,VP)在不同食品接触材料(聚苯乙烯,玻璃和不锈钢)表面生物被膜形成情况,并用蛋白质组学的方法探究不同材料表面形成差异生物被膜的初步机制。结果表明,VP在玻璃表面生物被膜形成能力最强,聚苯乙烯表面次之,不锈钢表面最弱。借助二维电泳技术找到156个差异蛋白点。GO富集分析表明这些差异蛋白主要与细菌的生物过程、细胞定位和分子功能相关,生物过程主要体现在小分子及单细胞代谢过程,细胞定位主要体现在细胞及细胞液上,分子功能集中于结合蛋白和金属阳离子方面,使细胞不断伸长、分泌胞外聚合物并结合周围金属阳离子形成骨架结构以粘附于不同载体表面。蛋白相互作用分析发现伴侣蛋白DnaK和外膜蛋白TolC在VP生物被膜的形成中有重要的调控作用。本研究为不同食品接触材料表面VP生物被膜的形成机制提供了理论基础。
Crystal violet staining and scanning electron microscope were used to compare the biofilm formation ability of Vibrio parahaemolyticus at different food contact materials(polystyrene,glass and stainless steel),and proteomics were analyzed to investigate the mechanism of discrepant biofilm formation at these surfaces.The results showed that V.parahaemolyticus exhibited strongest biofilm-forming ability at glass,polystyrene is next and the last is stainless steel.In total,156 differential protein were detected based on the two dimensional electrophoresis.Gene Ontology(GO) analysis indicated that these differentially expressed proteins are mainly related to biological process,cellular component and molecular function.When V.parahaemolyticus formed discrepant biofilm on three contact surfaces,small molecule metabolic and single-organism metabolic process play a leading role in biological process,cell components were mainly reflected by cytosol and cytoplasmic,and molecular function were primarily embodied with binding protein and metal positive ions.Because of these,bacteria could undergo dramatic elongation,secretion of extracellular polymeric substances and bind metal positive ions for attaching to different contact surfaces.Protein-protein interaction analysis indicated that chaperone protein DnaK and outer membrane protein TolC may regulate the process of biofilm formation by V.parahaemolyticus on various food contact surfaces.This study provides theoretical basis for the underlying mechanism of V.parahaemolyticus biofilm formation at different contact materials.
Crystal violet staining and scanning electron microscope were used to compare the biofilm formation ability of Vibrio parahaemolyticus at different food contact materials(polystyrene,glass and stainless steel),and proteomics were analyzed to investigate the mechanism of discrepant biofilm formation at these surfaces.The results showed that V.parahaemolyticus exhibited strongest biofilm-forming ability at glass,polystyrene is next and the last is stainless steel.In total,156 differential protein were detected based on the two dimensional electrophoresis.Gene Ontology(GO) analysis indicated that these differentially expressed proteins are mainly related to biological process,cellular component and molecular function.When V.parahaemolyticus formed discrepant biofilm on three contact surfaces,small molecule metabolic and single-organism metabolic process play a leading role in biological process,cell components were mainly reflected by cytosol and cytoplasmic,and molecular function were primarily embodied with binding protein and metal positive ions.Because of these,bacteria could undergo dramatic elongation,secretion of extracellular polymeric substances and bind metal positive ions for attaching to different contact surfaces.Protein-protein interaction analysis indicated that chaperone protein DnaK and outer membrane protein TolC may regulate the process of biofilm formation by V.parahaemolyticus on various food contact surfaces.This study provides theoretical basis for the underlying mechanism of V.parahaemolyticus biofilm formation at different contact materials.
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