基于化学蛋白质组学的黄芩苷抗肝脏脂肪化机制研究
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- 英文论文题名：Research on Baicalin's anti-steatosis effects in hepatocyte based on chemoproteomics
- 论文作者：戴建业 ; 王初
- 英文论文作者：Jianye Dai ; Chu Wang ; Department of Chemical Biology ; Peking University
- 年：2016
- 作者机构：北京大学化学生物学系;
- 论文关键词：化学蛋白质组学 ; 肝脏脂肪化 ; 黄芩苷
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第二十八分会：化学生物学
- 语种：中文
- 分类号：R285
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第二十八分会 ; 化学生物学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：301k
- 原文格式：D
- 会议级别：全国
摘要
脂肪性肝病是世界最为常见的疾病之一。它不仅包括单纯性肝脏脂肪化,而且会进一步的发展为脂肪性肝炎、肝硬化、纤维化甚至肝癌。在脂肪性肝病的疾病进程中,肝脏脂肪化是其共同特征。然而现阶段主流医学并没有针对肝脏脂肪化的有效的治疗方法。随着传统中医药在世界健康体系中发挥了越来越重要的作用,其安全性和有效性得到越来越多的认可。在传统中药中,黄芩作为治疗肝脏疾病的常用药引起了大家的注意。现代药理学证明,黄芩具有抗脂肪肝、抗炎、抗氧化、抗纤维化等作用。黄芩苷作为黄芩中最主要活性成分,在其抗脂肪肝的疗效中发挥着重要作用。虽然已有大量研究对于黄芩苷作用机制进行了探讨,但是对于其直接作用靶点的系统研究未见报道。因此,我们利用化学蛋白质组学的方法对于黄芩苷抗脂质化的机制进行了系统研究。最终发现,黄芩苷是通过调节脂肪酸β氧化发挥其抗脂质化作用的。
Fatty Liver Disease(FLD) is one of the most prevalent liver diseases in modern society,which is characteristic of steatosis.It starts from simple steatosis to more advanced stages,such as steatohepatitis,fibrosis,cirrhosis,and even hepatocellular carcinoma.Mainstream medicine has made many endeavors,however there's no effective treatment yet,unfortunately.Traditional Chinese Medicine(TCM) with the multi-target characteristics have been used for thousands years to treat FLD related diseases,the mechanism of which still remains elusive.In this work,we confirmed the anti-hepatic steatosis effect of Baicalin,an active ingredient,from Scutellaria Baicalensis,then performed the activity-based protein profiling(ABPP) to find out Baicalin's direct targets.With further functional study,,we demonstrated that Baicalin has multi-target effects in mitochondria fatty acid β-oxidation.
Fatty Liver Disease(FLD) is one of the most prevalent liver diseases in modern society,which is characteristic of steatosis.It starts from simple steatosis to more advanced stages,such as steatohepatitis,fibrosis,cirrhosis,and even hepatocellular carcinoma.Mainstream medicine has made many endeavors,however there's no effective treatment yet,unfortunately.Traditional Chinese Medicine(TCM) with the multi-target characteristics have been used for thousands years to treat FLD related diseases,the mechanism of which still remains elusive.In this work,we confirmed the anti-hepatic steatosis effect of Baicalin,an active ingredient,from Scutellaria Baicalensis,then performed the activity-based protein profiling(ABPP) to find out Baicalin's direct targets.With further functional study,,we demonstrated that Baicalin has multi-target effects in mitochondria fatty acid β-oxidation.
引文
[1]Peng,XD.;Dai,LL.;Huang,CQ.;He,CM;Chen,LJ.World J Gastroenterol 2009,15(37):4720.
[2]Lu,MF.;Xiao,ZT.;Zhang,HY.Biochem Biophys Res Commun.2013,434(4):701.
[2]Lu,MF.;Xiao,ZT.;Zhang,HY.Biochem Biophys Res Commun.2013,434(4):701.