以氮硫杂化的碳量子点为MALDI新型基质对小分子的检测
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- 英文论文题名：N,S-doped carbon dots as a novel matrix for the analysis of small molecules by MALDI-TOF MS
- 论文作者：王彦伟 ; 陈永丽 ; 高丹 ; 刘红霞
- 英文论文作者：Yanwei Wang ; Yongli Chen ; Dan Gao ; Hongxia Liu ; Graduate School at Shenzhen ; Tsinghua University ; The Key Laboratory of Chemical Biology
- 年：2016
- 作者机构：清华大学深圳研究生院广东省化学生物学重点实验室;
- 论文关键词：N ; S-CDs ; MALDI-TOFMS ; 基质 ; 小分子检测
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第四十三分会：质谱分析
- 语种：中文
- 分类号：O657.63;R115
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第四十三分会 ; 质谱分析
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：153k
- 原文格式：D
- 会议级别：全国
摘要
近年来,基质辅助激光解析电离飞行时间质谱(MALDI-TOFMS)由于其样品前处理简单、高盐耐受度以及快速高通量数据采集能力等优势而成为越来越重要的分析工具。受限于现有基质检测时的背景基质干扰以及低重现性,目前MALDI-TOF质谱很少用于检测小分子代谢物。本课题采用水热反应法合成一种新基质N,S-CDs,并将其应用于小分子检测。通过对N,S-CDs进行结构形态表征,并与常用基质9-AA、CHCA等对小分子代谢物如氨基酸、多肽、核苷在正负离子模式下的检测结果进行对比。结果显示N,S-CDs相对于9-AA、CHCA等基质,能更好地提高检测灵敏度,并且无基质干扰峰,其耐盐能力、重现性也相对较好。此外,我们将这一新基质应用于血清样本的检测以及不同亚型乳腺癌细胞的分类,其结果显示我们合成的新基质能很好地检测出复杂生物样本中的内源性代谢物小分子,并实现了肿瘤细胞更明显的分类。综上,本研究合成的N,S-CDs在代谢组学、生物标记物发现、环境分析等领域将会有较大的应用潜力。
Recently,the application of MALDI-TOF MS in low-molecular-weight analytes detection was restricted by the existing matrices due to the matrix-related peaks and low reproducibility.Here,a new matrix N,S-CDs,for the first time,was synthesized and used in small molecule analysis.Comprehensive assessments of N,S-CDs were conducted in this work,and the results showed that N,S-CDs could be a sensational matrix which enhanced the signal intensity of the analytes without matrix-related interference,especially when it was used in negative ion mode.The application of N,S-CDs in serum analysis and classification the subtypes of breast cancer cell lines indicated the ability of our new matrix to detect endogenous metabolites in complex biological sample.In summary,the synthesized N,S-CDs has the potential to be applied in metabolomics,biomakers and environmental analysis.
Recently,the application of MALDI-TOF MS in low-molecular-weight analytes detection was restricted by the existing matrices due to the matrix-related peaks and low reproducibility.Here,a new matrix N,S-CDs,for the first time,was synthesized and used in small molecule analysis.Comprehensive assessments of N,S-CDs were conducted in this work,and the results showed that N,S-CDs could be a sensational matrix which enhanced the signal intensity of the analytes without matrix-related interference,especially when it was used in negative ion mode.The application of N,S-CDs in serum analysis and classification the subtypes of breast cancer cell lines indicated the ability of our new matrix to detect endogenous metabolites in complex biological sample.In summary,the synthesized N,S-CDs has the potential to be applied in metabolomics,biomakers and environmental analysis.
引文
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