草鱼NgR1基因的克隆及表达分析
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- 英文论文题名：Cloning and expression analysis of the NgR1 gene in grass carp(Ctenopharyngodon idellus)
- 论文作者：焦真真 ; 田园园 ; 叶星
- 英文论文作者：JIAO Zhenzhen ; TIAN Yuanyuan ; YE Xing ; Key Laboratory of Tropical&Subtropical Fisheries Resource Application&Cultivation of China Ministry of Agriculture ; Pearl River Fisheries Institute ; Chinese Academy of Fishery Sciences ; Guangzhou ; College of Fisheries and Life Science ; Shanghai Ocean University
- 年：2016
- 作者机构：农业部热带亚热带水产资源利用与养殖重点实验室珠江水产研究所中国水产科学研究院;上海海洋大学水产与生命技术学院;
- 论文关键词：草鱼 ; NgR1 ; 整胚原位杂交 ; 组织表达模式 ; 呼肠孤病毒 ; 病毒受体
- 英文论文关键词：Grass carp ; NgR1 ; gene cloning ; tissue expression ; reovirus ; virus receptor
- 会议召开时间：2016-11-02
- 会议录名称：2016年中国水产学会学术年会论文摘要集
- 语种：中文
- 分类号：S917.4
- 学会代码：OGSB
- 会议名称：2016年中国水产学会学术年会
- 会议地点：中国四川成都
- 主办单位：中国水产学会、四川省水产学会
- 学会名称：中国水产学会
- 页数：1
- 文件大小：533k
- 原文格式：D
- 会议级别：全国
摘要
NgR1(Nogo-66 receptor)是在哺乳类上发现的一种神经元受体,可调节轴突可塑性并抑制损伤CNS的再生。本研究在草鱼脑组织中克隆到NgR1(GcNgR1)的cDNA序列,其ORF长1443bp,编码480个氨基酸。分析发现其氨基酸序列上具有NgR1保守结构域—亮氨酸重复序列(LRR);qRT-PCR检测该基因的组织分布,发现该基因在健康草鱼脑中高表达,在鳃、肝等其它8个组织中的表达量均较低;早期胚胎发育中GcNgR1的表达,发现在未受精卵中可检测到GcNgR1的表达,受精卵至孵化出膜第1天(1 dph)幼鱼GcNgR1的表达量均较低;2 dph开始表达量大幅升高;整胚原位杂交结果也显示在2 dph GcNgR1在脑中高表达,而在胚胎发育早期(肌节出现期和心脏效应期)信号不明显。以草鱼呼肠孤病毒GCRV-GD108株攻毒后,草鱼鳃、肝等组织中的NgR1表达量都有不同程度的升高,但以脑中的表达上升最显著;GCRV-GD108感染草鱼成纤维细胞后,攻毒第4天GcNgR1的表达量出现显著上升趋势,与病毒增殖趋势一致。本研究结果表明,GcNgR1在草鱼脑组织中高表达,受GCRV时感染表达显著上调,推测GcNgR1极可能是GCRV的神经元受体分子。
Nogo-66 receptor(NgR1), expresses in neuron surface, can inhibit the regeneration of injury CNS and regulate the axon plasticity in mammals. In this study, the cDNA sequence of the grass carp NgR1(GcNgR1) was cloned. Its ORF was 1443 bp in length, encoding 480 amino acids.Domain analysis shows it possesses conserved NgR1 domain LRRs in its amino acid sequence.qRT-PCR were performed to detect its expression patterns in embryonic and juvenile. Expression level of GcNgR1 was higher in the brain than in other tissues. It is expressed in the early embryo development stages, with a significant rise at 2 dph, which was verified by in situ hybridization.After challenged with GCRV-GD108, expression of GcNgR1 showed more significant increase in brain than other tissues.
Nogo-66 receptor(NgR1), expresses in neuron surface, can inhibit the regeneration of injury CNS and regulate the axon plasticity in mammals. In this study, the cDNA sequence of the grass carp NgR1(GcNgR1) was cloned. Its ORF was 1443 bp in length, encoding 480 amino acids.Domain analysis shows it possesses conserved NgR1 domain LRRs in its amino acid sequence.qRT-PCR were performed to detect its expression patterns in embryonic and juvenile. Expression level of GcNgR1 was higher in the brain than in other tissues. It is expressed in the early embryo development stages, with a significant rise at 2 dph, which was verified by in situ hybridization.After challenged with GCRV-GD108, expression of GcNgR1 showed more significant increase in brain than other tissues.
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