斑马鱼mc4r对能量代谢和性腺发育影响的分子机制研究
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- 英文论文题名：The effect and underlying mechanism of mc4r on the energy metabolism and gonad development in zebrafish
- 论文作者：王超 ; 边万平 ; 谢少林 ; 邹记兴 ; 裴得胜
- 英文论文作者：WANG Chao ; BIAN Wanping ; XIE Shaolin ; ZOU Jixing ; PEI Desheng ; College of Marine Sciences ; South China Agricultural University ; Chongqing Institute of Green and Intelligent Technology ; Chinese Academy of Sciences
- 年：2016
- 作者机构：华南农业大学海洋学院;中国科学院重庆绿色智能技术研究院环境与健康研究中心;
- 论文关键词：Mc4r ; CRISPR/Cas9 ; 性腺发育 ; 能量代谢 ; 斑马鱼
- 英文论文关键词：mc4r ; CRISPR/Cas9 ; energy metabolism ; gonad development ; zebrafish
- 会议召开时间：2016-11-02
- 会议录名称：2016年中国水产学会学术年会论文摘要集
- 语种：中文
- 分类号：S917.4
- 学会代码：OGSB
- 会议名称：2016年中国水产学会学术年会
- 会议地点：中国四川成都
- 主办单位：中国水产学会、四川省水产学会
- 学会名称：中国水产学会
- 页数：1
- 文件大小：561k
- 原文格式：D
- 会议级别：全国
摘要
黑素皮质素受体-4(mc4r)是下丘脑分泌的具有调节摄食和维持体内能量稳态的一类肽类物质。为了研究mc4r基因在能量平衡中的调控机制,我们通过CRISPR/Cas9技术构建了mc4r基因缺失斑马鱼模型,并对其生长和繁殖力等参数与野生型斑马鱼进行了比较研究;同时通过实时荧光定量PCR检测了斑马鱼性腺发育以及能量调节信号通路的相关基因表达情况。生理测试结果显示mc4r缺失斑马鱼出现了摄食增加、繁殖力增强现象,具体表现在产卵量显著增加。雌鱼和雄鱼大脑中调控性腺发育的基因leptin、kisspeptin、gnrh和adcyap1a在纯合子中表达显著上调。在性腺中,促性腺发育相关基因fshβ、lhβ和lhcgr在纯合子中同样表达上调,而负调控基因bmp15表达量显著下调。本实验初步证明了在斑马鱼中mc4r基因参与了调节摄食和能量代谢平衡,并且能够明显的促进斑马鱼的性腺发育并提高其繁殖力。该研究初步阐释了mc4r在内分泌调节作用机理,为深入揭示mc4r与生殖之间的调控机制奠定了基础。
Melanocortin receptor-4(MC4R), which is secreted by the hypothalamus, can regulate food intake and maintain energy homeostasis. We constructed mc4 r deletion zebrafish by CRISPR/Cas9 technology for studying the mechanism of mc4 r in energy balance regulation. A comprehensive comparative study was performed on various aspects of growth and fertility between deletion and wild type zebrafish and the expression of genes involed in energy metabolism and gonad development were analysed. The results show that both heterozygous and homozygous appeared food intake increased and fertility enhancement. The expression of genes leptin, kisspeptin, gnrh3, adcyap1 a, fsh β, lh β and lhcgr, which were regarded to enhance fecundity, were notable up-regulated in brain and gonad of homozygous zebrafish. Concomitantly,the expression of bmp15 was inhibited, which was a negative regulator. The result indicated that mc4 r involved in food intake regulation, energy balance and gonad development in zebrafish. This work will be a foundation for the further study of the mechanism between mc4 r and reproductive regulation.
Melanocortin receptor-4(MC4R), which is secreted by the hypothalamus, can regulate food intake and maintain energy homeostasis. We constructed mc4 r deletion zebrafish by CRISPR/Cas9 technology for studying the mechanism of mc4 r in energy balance regulation. A comprehensive comparative study was performed on various aspects of growth and fertility between deletion and wild type zebrafish and the expression of genes involed in energy metabolism and gonad development were analysed. The results show that both heterozygous and homozygous appeared food intake increased and fertility enhancement. The expression of genes leptin, kisspeptin, gnrh3, adcyap1 a, fsh β, lh β and lhcgr, which were regarded to enhance fecundity, were notable up-regulated in brain and gonad of homozygous zebrafish. Concomitantly,the expression of bmp15 was inhibited, which was a negative regulator. The result indicated that mc4 r involved in food intake regulation, energy balance and gonad development in zebrafish. This work will be a foundation for the further study of the mechanism between mc4 r and reproductive regulation.
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