不同时期冷藏金枪鱼优势腐败菌多样性及生物胺变化规律的研究
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- 英文论文题名：Changes of diversity of dominant spoilage bacteria and biogenic amines in tuna(Thunnus obesus) from different growing seasons during chilled storage
- 论文作者：刘爱芳 ; 钱韻芳 ; 谢晶 ; 邓添
- 英文论文作者：Liu Aifang ; Qian Yunfang ; Xie Jing ; Deng Tian ; Shanghai Engineering Research Center of Aquatic Product Processing and preservation.College of Food Science and Technology ; Shanghai Ocean University
- 年：2016
- 作者机构：上海水产品加工及贮藏工程技术研究中心上海海洋大学食品学院;
- 论文关键词：优势腐败菌 ; 冷藏 ; 金枪鱼 ; 聚合酶链式反应(PCR) ; 生理生化鉴定 ; 生物胺
- 英文论文关键词：dominant spoilage bacteria ; chilled storage ; tuna ; microbial flora ; polymerase chain reaction(PCR) ; biogenic amines
- 会议召开时间：2016-11-09
- 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
- 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
- 语种：中文
- 分类号：TS254.1
- 学会代码：ZGSP
- 会议名称：中国食品科学技术学会第十三届年会
- 会议地点：中国北京
- 主办单位：中国食品科学技术学会
- 学会名称：中国食品科学技术学会
- 页数：1
- 文件大小：54k
- 原文格式：O
- 会议级别：全国
摘要
研究了不同时期冷藏金枪鱼优势腐败菌多样性及生物胺的变化规律。通过生理生化鉴定、16S rRNA序列分析鉴定及系统发育树的建立,以及生物胺(腐胺Put、组胺His、酪胺Tyr)的检测,分析得到4℃冷藏8天内的金枪鱼菌优势腐败菌多样性及生物胺的变化规律,并最终分离筛选到21株不同特征的纯化菌株。结果表明,随着贮藏时间的延长,金枪鱼的生物胺含量逐渐升高,其中组胺含量最高,而酪胺含量始终处于低水平状态;冷藏金枪鱼初始菌相主要构成为嗜油不动杆菌(Acinetobacter oleivorans)、莓实假单胞菌(Pseudomonas fragi)、热死环丝菌(Brochothrix thermosphacta)、黄歇假单胞菌(Pseudomonas fulva);微生物菌落结构在贮藏期间不断变化,多样性降低,在货架期第8天逐渐形成以不动杆菌(Acinetobacter spp.)、假单胞菌(Pseudomonas spp.)为主的微生物群落结构。
The changes of dominant microflora and biogenic amines of tuna(Thunnus obesus) during chilled storage were studied by henotypic analysis 16 S rRNA gene sequencing analysis and the establishment of phylogenetic tree,and the content of biogenic amines(putrescine,histamine,tyramine) changes of tuna were researched regularly during iced storage.Finally,21 bacteria strains of tuna stored under 4℃ were obtained.The results showed that the content of biogenic amines were increased with the extension of storage time,and histamine content were increased rapidly and became the major biogenic amines,while tyramine was low in all storage time.The initial microbial flora was dominated by Acinetobacter oleivorans,Pseudomonas fragi,Brochothrix thermosphacta,Pseudomonas fulva.The structure of microbial growth kept changing during storage,and the bacterial diversity of tuna decreased along with storage time.On the shelf-life of eighth day Acinetobacter spp.,Pseudomonas spp.,Brochothrix thermosphacta became the dominant bacteria in the microbial community.
The changes of dominant microflora and biogenic amines of tuna(Thunnus obesus) during chilled storage were studied by henotypic analysis 16 S rRNA gene sequencing analysis and the establishment of phylogenetic tree,and the content of biogenic amines(putrescine,histamine,tyramine) changes of tuna were researched regularly during iced storage.Finally,21 bacteria strains of tuna stored under 4℃ were obtained.The results showed that the content of biogenic amines were increased with the extension of storage time,and histamine content were increased rapidly and became the major biogenic amines,while tyramine was low in all storage time.The initial microbial flora was dominated by Acinetobacter oleivorans,Pseudomonas fragi,Brochothrix thermosphacta,Pseudomonas fulva.The structure of microbial growth kept changing during storage,and the bacterial diversity of tuna decreased along with storage time.On the shelf-life of eighth day Acinetobacter spp.,Pseudomonas spp.,Brochothrix thermosphacta became the dominant bacteria in the microbial community.
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