HPLC法测定盐酸左旋咪唑粉中盐酸左旋咪唑的含量
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- 英文论文题名：Determination ofLevamisoleHydrochloride in LevamisoleHydrochloride Powder by HPLC
- 论文作者：王丽 ; 刘素梅 ; 藏合英 ; 赵晨宇 ; 韩立 ; 邱天宝
- 英文论文作者：WANG Li ; LIU Su-mei ; ZANG He-ying ; ZHAO Chen-yu ; HAN Li ; QIU Tian-bao ; Henan Province Institute of Veterinary Drug and Feed Control ; Chongqing Medical University
- 年：2016
- 作者机构：河南省兽药饲料监察所;重庆医科大学;
- 论文关键词：盐酸左旋咪唑粉 ; 高效液相色谱法 ; 盐酸左旋咪唑
- 英文论文关键词：Levamisole hydrochloride powder ; HPLC ; Levamisole hydrochloride
- 会议召开时间：2016-09-18
- 会议录名称：中国畜牧兽医学会动物药品学分会第五届全国会员代表大会暨2016年学术年会论文集
- 语种：中文
- 分类号：S859.79;O657.72
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物药品学分会第五届全国会员代表大会暨2016年学术年会
- 会议地点：中国四川成都
- 主办单位：中国畜牧兽医学会动物药品学分会
- 学会名称：中国畜牧兽医学会
- 页数：5
- 文件大小：292k
- 原文格式：O
- 会议级别：全国
摘要
建立盐酸左旋眯唑粉中盐酸左旋咪唑含量的高效液相色谱测定法。以0.05mol/L磷酸二氢钾(用三乙胺调节pH值为7)-乙腈(80:20)为流动相,盐酸左旋咪唑经C18色谱柱分离,在214nm波长下检测。试验结果表明,盐酸左旋咪唑在0.1~2.0mg/ml范围内线性关系良好,R~2=0.9995,平均回收率为101.3%(n=6),RSD为0.6%。本方法简便、准确、重现性好,可更好的控制盐酸左旋咪唑粉的质量。
The method for determination of levamisole hydrochloride inlevamisole hydrochloride powder by HPLC was developed.Theanalytewas separated on C18 chromatographiccolumn using a mobile phase of potassium dihydrogen phosphate(0.05mol/L,pH value adjusted to 7.0 withtriethylamine)-acetonitrile(80:20),and detected at the wavelength of 214 nm.The method was validated foe specificity,accuracy,linearity,precision and robustness.The results showed that a good linear relationship was accepted in the range of 0.1~2.0mg/ml(R~2=0.9995),and the average recovery was 101.3%(n=6),and the RSD value was 0.6%.This methodwas suited to control the quality of levamisole hydrochloride powder.
The method for determination of levamisole hydrochloride inlevamisole hydrochloride powder by HPLC was developed.Theanalytewas separated on C18 chromatographiccolumn using a mobile phase of potassium dihydrogen phosphate(0.05mol/L,pH value adjusted to 7.0 withtriethylamine)-acetonitrile(80:20),and detected at the wavelength of 214 nm.The method was validated foe specificity,accuracy,linearity,precision and robustness.The results showed that a good linear relationship was accepted in the range of 0.1~2.0mg/ml(R~2=0.9995),and the average recovery was 101.3%(n=6),and the RSD value was 0.6%.This methodwas suited to control the quality of levamisole hydrochloride powder.
引文
[1]兽药国家标准汇编—兽药地方标准上升国家标准第二册[S].
[2]刘素梅,李华岑,韩立等.万乳康中盐酸左旋眯唑含量测定方法的研究[J]冲国兽药杂志,2010,44(2):26-28.
[3]曾建亭,钟小燕.HPLC法测定盐酸左旋咪唑片的含量及溶出度[J].中国热带医学,2010,10(5):628-629.
[4]徐灿辉,何维为.高效液相色谱法测定盐酸左旋咪唑片的含量[J].药物鉴定,2007,16(7):12~13.
[5]王勇忠,高效液相色谱法测定盐酸左旋咪唑搽剂的含量[J].海峡药学,2007,19(12):52~53.
[6]陆兴毅.HPLC法测定盐酸左旋咪唑糖浆的含量[J].药物分析杂志,2008,28(10):1753~1754.
[2]刘素梅,李华岑,韩立等.万乳康中盐酸左旋眯唑含量测定方法的研究[J]冲国兽药杂志,2010,44(2):26-28.
[3]曾建亭,钟小燕.HPLC法测定盐酸左旋咪唑片的含量及溶出度[J].中国热带医学,2010,10(5):628-629.
[4]徐灿辉,何维为.高效液相色谱法测定盐酸左旋咪唑片的含量[J].药物鉴定,2007,16(7):12~13.
[5]王勇忠,高效液相色谱法测定盐酸左旋咪唑搽剂的含量[J].海峡药学,2007,19(12):52~53.
[6]陆兴毅.HPLC法测定盐酸左旋咪唑糖浆的含量[J].药物分析杂志,2008,28(10):1753~1754.