核酸适配体修饰的石墨烯量子点构建荧光传感器检测赭曲霉素A
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- 英文论文题名：Fluorescence biosensor based on aptmater modified graphene quantum dots for the detection of OTA
- 论文作者：汪松 ; 张瑛洧
- 英文论文作者：Song Wang ; Yingwei Zhang ; State Key Laboratory of Chemical Resource Engineering ; Beijing University of Chemical Technology
- 年：2016
- 作者机构：北京化工大学化工资源有效利用国家重点实验室;
- 论文关键词：石墨烯量子点 ; 赭曲霉素A ; 核酸适配体 ; 荧光传感器
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第三十六分会：纳米材料合成与组装
- 语种：中文
- 分类号：TB383.1;O657.3
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第三十六分会 ; 纳米材料合成与组装
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：185k
- 原文格式：D
- 会议级别：全国
摘要
传统的适配体荧光传感器通常需要荧光分子对探针进行修饰,同时引入额外的淬灭剂材料关闭荧光信号。由于修饰的荧光分子其光稳定性较差,淬灭剂材料的引入增加了操作的繁琐性,因而需要构建一种新型的适配体荧光传感器。本文以光学稳定性及生物相容性都较好的石墨烯量子点作为荧光信号基团,修饰到核酸适配体的一端,利用石墨烯量子点在聚集和解聚状态下荧光信号的淬灭及恢复,构建了一种无需荧光猝灭剂的新型荧光传感器。分别在OTA适配体DNA以及与其互补的短链探针DNA上修饰石墨烯量子点,当这两种DNA混合杂交时,石墨烯量子点近距离接触,发生激子能量转移和非辐射共振能量转移~[1],导致量子点荧光信号淬灭。加入目标分析物OTA后,其与适配体DNA特异性结合,替换下的短链探针DNA脱离,引起石墨烯量子点聚集体解组装而重新分散,体系荧光强度恢复。该方法的线性检测范围为0.005ng/m L~(-1)ng/mL,检测下限为0.004ng/m L,且具有很高的特异性检测能力。
we demonstrate an sensitive OTA detection method based on an extremely simple probe with only fluorogens but without quencher groups. It avoids complex and difficult steps to accurately design the relative distance between the fluorogens and quencher groups in the probes. The strategy was inspired by some recent works on self-aggregation and disaggregation for the detection of glucose, protein and metal ions. It was confirmed that two kinds of grapheme quantum dots modified by complementary DNA through specific DNA hybridization forms self-assembled aggregates in aqueous solution(signal off) and that the aggregates disassemble in response to the OTA added in system through the specific aptamer–OTA interaction(signal on). This switching mechanism based on the self-aggregation and disaggregation for the determination of OTA is considered to be unique. The new optical biosensor has many advantages such as high sensitivity and very low detection limit without any quencher groups.
we demonstrate an sensitive OTA detection method based on an extremely simple probe with only fluorogens but without quencher groups. It avoids complex and difficult steps to accurately design the relative distance between the fluorogens and quencher groups in the probes. The strategy was inspired by some recent works on self-aggregation and disaggregation for the detection of glucose, protein and metal ions. It was confirmed that two kinds of grapheme quantum dots modified by complementary DNA through specific DNA hybridization forms self-assembled aggregates in aqueous solution(signal off) and that the aggregates disassemble in response to the OTA added in system through the specific aptamer–OTA interaction(signal on). This switching mechanism based on the self-aggregation and disaggregation for the determination of OTA is considered to be unique. The new optical biosensor has many advantages such as high sensitivity and very low detection limit without any quencher groups.
引文
[1]Liu,J.;Liu,Y.;Yang,X.;Wang,K.;Wang,Q.;Shi,H.;Li,L.Anal.Chem.2013,85,11121-11128.