杂化硅胶整体柱固定化木瓜蛋白酶反应器的制备及应用
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摘要
<正>固定化酶反应器由于兼具固定化酶的特异性催化、低消耗、易分离和可重复利用等优点,在生物催化、蛋白质组学、酶抑制剂筛选、食品处理、污水处理等领域得到了快速发展1,2。由于整体柱压力小、通透性好、传质快和易功能化等优点,毛细管整体柱固定化酶的研究引起人们极大兴趣3。为了提高酶处理量,本工作在不锈钢色谱柱中构建固定化酶反应器。首先采用有机硅烷制备了杂化硅胶整体柱,然后用环氧基硅烷进行原位修饰,通过环氧基的开
引文
1.G.Shen,W.Yu,M.Liu,et al.,Preparation and application of immobilized enzyme micro-reactor,Prog.Chem.,2013,25:1198-1207
2 .C.Verdnyn,A.Suksomeheep,M.Suphnatharika,Effect of high pressure homogenization and papain on the preparation of autolysed yeast extract,World J.Microbiol.Biotechnol.,1999,15(l):57-63
3 .J.Ma,Z.Liang,X.Qiao,et al.,Organic-inorganic hybrid silica monolith based immobilized trypsin reactor with high enzymatic activity,Anal.Chem.,2008,80:2949-2956
2 .C.Verdnyn,A.Suksomeheep,M.Suphnatharika,Effect of high pressure homogenization and papain on the preparation of autolysed yeast extract,World J.Microbiol.Biotechnol.,1999,15(l):57-63
3 .J.Ma,Z.Liang,X.Qiao,et al.,Organic-inorganic hybrid silica monolith based immobilized trypsin reactor with high enzymatic activity,Anal.Chem.,2008,80:2949-2956