葛根纤维低聚糖的酶解制备及其对乳酸菌的体外促生长作用
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- 英文论文题名：The enzymatic preparation of Radix Puerariae oligosaccharides and itsgrowth-promoting effectson Lactic acid bacteria in Vitro
- 论文作者：张雁 ; 刘松珍 ; 池建伟 ; 唐小俊 ; 张名位
- 英文论文作者：Zhang Yan ; Liu Song-zhen ; Chi Jianwei ; Tang Xiaojun ; Zhang Mingwei ; Sericultural & Agri-Food Research Institute Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods ; Ministry of Agriculture/Guangdong Key Laboratory of Agricultural Products Processing
- 年：2016
- 作者机构：广东省农业科学院蚕业与农产品加工研究所/农业部功能食品重点实验室/广东省农产品加工重点实验室;
- 论文关键词：葛根 ; 纤维低聚糖 ; 酶解 ; 乳酸菌 ; 促生长作用
- 英文论文关键词：radix puerariae ; fiber oligosaccharides ; enzymolysis ; lactic acid bacteria ; growth promoting effects
- 会议召开时间：2016-11-09
- 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
- 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
- 语种：中文
- 分类号：TS201.3
- 学会代码：ZGSP
- 会议名称：中国食品科学技术学会第十三届年会
- 会议地点：中国北京
- 主办单位：中国食品科学技术学会
- 学会名称：中国食品科学技术学会
- 页数：2
- 文件大小：111k
- 原文格式：O
- 会议级别：全国
摘要
葛根富含淀粉、异黄酮和膳食纤维,其中的淀粉和异黄酮已得到较好利用,而大量膳食纤维作为垃圾丢弃。本研究采用β-葡聚糖酶酶解葛根膳食纤维制备纤维低聚糖;并以葛根纤维低聚糖为碳源,探讨其对肠道益生菌的体外促生长作用。采用Box-Benhnken中心组合试验设计优化得到葛根纤维低聚糖酶解制备工艺:酶用量5U/g,pH5.8,酶解时间3.0hr,温度48℃;此优化条件下,酶解产物中葡萄糖质量浓度最低,为0.49mg/mL,纤维低聚糖质量浓度可达到2.07mg/mL,其中纤维二糖1.36mg/mL,纤维三糖为0.71mg/mL。以葛根纤维低聚糖完全取代乳酸菌基础培养基中的碳源,培养24hr后,保加利亚乳杆菌、嗜热链球菌及青春双歧杆菌活菌数分别比基础培养基提高123%,124%and 146%。
Radix Puerariae is richedin isoflavonoids,starch and dietary fiber.Itis mainly used for production of starch and isoflavonoids nowadays.But plenty of dietary fiberwasignored.In the present work,cellooligosaccharides were prepared from Radix Puerariae dietary fiber by β-glucanase.By using Radix Puerariae cellooligosaccharidesas carbon source substitute in microbiological culture media,its growth-promoting effects on lactic acid bacteria in Vitro were also investigated.The preparing conditionsof Radix Puerariae cellooligosaccharides were optimized by Box-Benhnken design:β-glucanaseaddtion 5U/g,enzymolysis pH5.8,enzymolysis time 3.0 hr,enzymolysis temperature 48 ℃.Under these conditions,the glucose concentration in hydrolysatewas 0.49mg/mL,while the cellooligosaccharideconcentration was 2.07mg/mL,in which,cellobiose was1.36mg/mL,cellotriose was 0.71mg/mL.When carbon sourcein microbiological culture media was totally substituted by Radix Puerariae cellooligosaccharides,the number of Bulgaria Lactobacillus,Thermophilic streptococcus and Bifidobacterium adolescentis were increased by 123%,124%and 146%respectively 24 hr later.
Radix Puerariae is richedin isoflavonoids,starch and dietary fiber.Itis mainly used for production of starch and isoflavonoids nowadays.But plenty of dietary fiberwasignored.In the present work,cellooligosaccharides were prepared from Radix Puerariae dietary fiber by β-glucanase.By using Radix Puerariae cellooligosaccharidesas carbon source substitute in microbiological culture media,its growth-promoting effects on lactic acid bacteria in Vitro were also investigated.The preparing conditionsof Radix Puerariae cellooligosaccharides were optimized by Box-Benhnken design:β-glucanaseaddtion 5U/g,enzymolysis pH5.8,enzymolysis time 3.0 hr,enzymolysis temperature 48 ℃.Under these conditions,the glucose concentration in hydrolysatewas 0.49mg/mL,while the cellooligosaccharideconcentration was 2.07mg/mL,in which,cellobiose was1.36mg/mL,cellotriose was 0.71mg/mL.When carbon sourcein microbiological culture media was totally substituted by Radix Puerariae cellooligosaccharides,the number of Bulgaria Lactobacillus,Thermophilic streptococcus and Bifidobacterium adolescentis were increased by 123%,124%and 146%respectively 24 hr later.
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