DNA超分子水凝胶及其应用
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- 英文论文题名：DNA Hydrogels and their application in 3D Bioprinting
- 论文作者：刘冬生 ; 李闯 ; 程恩隽 ; 邢永政 ; 金娟
- 英文论文作者：Dongsheng Liu ; Chuang Li ; Enjun Cheng ; Yongzheng Xing ; Juan Jin ; Department of Chemistry ; Tsinghua University
- 年：2016
- 作者机构：清华大学化学系;
- 论文关键词：DNA ; 水凝胶 ; 超分子 ; 3D打印
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第二十四分会：超分子组装与软物质材料
- 语种：中文
- 分类号：O648.17
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第二十四分会 ; 超分子组装与软物质材料
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：163k
- 原文格式：D
- 会议级别：全国
摘要
利用DNA的高度特异性识别、结构可设计、生物相容性以及多种响应性,我们设计、制备了多种纯DNA以及多肽-DNA复合超分子水凝胶。这些凝胶具有优异的分子通透性、较高的力学强度、秒级成型、良好的触变性以及自愈合等特点;还可在化学组成、交联度、网络结构不改变的情况下,实现机械强度从10Pa到7000Pa的任意调整;在此基础上,我们将其应用于活细胞的3D打印和直写。
Based on sequence and structure design, we have prepared kinds of pure or hybrid DNA supramolecular hydrogels, which could be formed under physiological condition within a minute at room temperature and without using any organic solvents. By tailoring the length of "sticky ends" of DNA linker, mechanical property of the hydrogel could be varied from hundreds to thousands Pa(G', storage modulus); we also found that the viability of cell in a 4 mm diameter hydrogel is nearly 100% after 24 hours incubation from top in plastic tubes. These hydrogels possess extraordinary healing and fast-responding thixotropic properties, which make them injectable and writable. Because the formations of such hydrogels are based on DNA assembly, by DNA sequence design, they could be easily conferred excellent responsiveness including pH, DNA restriction enzymes, temperature etc., and enable easy removal after cell culture. In addition, we will show their application in 3D cell printing.
Based on sequence and structure design, we have prepared kinds of pure or hybrid DNA supramolecular hydrogels, which could be formed under physiological condition within a minute at room temperature and without using any organic solvents. By tailoring the length of "sticky ends" of DNA linker, mechanical property of the hydrogel could be varied from hundreds to thousands Pa(G', storage modulus); we also found that the viability of cell in a 4 mm diameter hydrogel is nearly 100% after 24 hours incubation from top in plastic tubes. These hydrogels possess extraordinary healing and fast-responding thixotropic properties, which make them injectable and writable. Because the formations of such hydrogels are based on DNA assembly, by DNA sequence design, they could be easily conferred excellent responsiveness including pH, DNA restriction enzymes, temperature etc., and enable easy removal after cell culture. In addition, we will show their application in 3D cell printing.
引文
1.C.Li,W.Shu and D.Liu et al.Angew.Chem.Int.Ed.,DOI:10.1002/anie.201411383
2.J.Jin,S.Wang and D.Liu et al.Advanced Materials,2013,25(34),4714.
3.D.Liu,E.Cheng and Z.Yang,NPG Asia Mater.,2011,3(10),109.
4.Y.Xing,D.Liu,et al.Advanced Materials,2011,23,1117.
2.J.Jin,S.Wang and D.Liu et al.Advanced Materials,2013,25(34),4714.
3.D.Liu,E.Cheng and Z.Yang,NPG Asia Mater.,2011,3(10),109.
4.Y.Xing,D.Liu,et al.Advanced Materials,2011,23,1117.