含银敷料表征、银的体外释放及其安全性评估
详细信息 查看官网全文
- 英文论文题名：The Characterization and Release Profiles of Ag Forms in the Silver-containing Wound Dressings: Implication for Safety Evaluation
- 论文作者：白茹 ; 徐丽明 ; 程祥 ; 陈春英
- 英文论文作者：Ru Bai ; Liming Xu ; Xiang Cheng ; Chunying Chen ; National Center for Nanoscience and Technology of China ; National Institutes for Food and Drug Control
- 年：2016
- 作者机构：国家纳米科学中心;中国食品药品检定研究院医疗器械检定所;
- 论文关键词：含银敷料 ; 纳米银 ; 银离子 ; 银释放 ; 风险评估
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第三十八分会：纳米生物效应与纳米药物化学
- 语种：中文
- 分类号：O614.122;TB383.1
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第三十八分会 ; 纳米生物效应与纳米药物化学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：2
- 文件大小：335k
- 原文格式：D
- 会议级别：全国
摘要
纳米银(AgNPs)被认为具有广谱的抗菌性,不易产生耐药,因而被广泛地应用于医疗领域。含银敷料作为应用最为广泛的医用材料之一,其所含银总量、银的存在形式和释放方法却缺乏规范性研究。本研究通过扫描电子显微镜(SEM)、透射电子显微镜(SEM)、X射线能量色散谱仪(EDS/EDX)、X射线光电子能谱仪(XPS)和X射线衍射仪(XRD)对含银敷料进行形貌及银颗粒价态分析表征,采用往复支架法研究含银敷料在模拟体液中的释放特性,通过体外细胞毒性研究,讨论材料表征与细胞毒性风险评价的相关性。结果表明,三种敷料均含有AgNPs,AT敷料呈现出致密、均一的特征,AgNPs尺寸为30-45 nm,为四面体结构,YB和Anson敷料呈纤维状,AgNPs散在分布,尺寸为5-20 nm;AT和YB敷料主要含有Ag~0,AS敷料含有Ag~0和Ag~+;三种敷料均释放出AgNPs和Ag~+,AT敷料银含量最高(1938.88±42.18μg/cm~2),AT敷料24小时内释放的总银含量(59.90±0.11μg/cm~2)及银纳米颗粒含量(29.04±0.12μg/cm~2)最高,但释放率仅为3%,可能与晶体结构及生产工艺有关。AT敷料银纳米颗粒释放量是YB敷料的2倍,是AS敷料的11倍;细胞毒性与AgNPs释放量正相关,即AT>YB>AS。本研究建立的含银表征和银释放特性的实验方法,能有效地判定敷料中是否含有纳米银及是否有纳米银颗粒的释放,为正确地对含银敷料进行风险评估及合理有效的监管提供科学依据。
Silver-containing wound dressings are now commonly available due to their advantage of broad-spectrum antimicrobial activities. However, few studies demonstrate their chemical composition, release kinetics and bioeffects, especially the strategies to distinguish and identify Ag-nanoparticle(Ag~0) and Ag-ion(Ag~+) forms. In this work, we have established systemic characterization and release profiles of various Ag forms in three commercial silver-containing wound dressings by combining several state-of-arts techniques, such as X-ray photoelectron spectroscope(XPS), transmission electron microscope(TEM), scanning electron microscopy with integrated energy dispersive X-ray spectroscopy(SEM-EDX), and ultrafiltration, etc. The results demonstrate that AT dressing is covered by condensed particles with size ranging from 30 to 45 nm, which mainly contains Ag~0 in face-centred cubic structure. YB and AS dressings show fibrous structures with 5~20 nm silver particles attached on the surface. YB dressing mainly contains Ag~0 and AS dressing contains both Ag~0 and Ag~+ forms. Both Ag nanoparticles and Ag ions can be found in simulated body fluids(SBF) after treating with the three dressings by the reciprocating holder method. AT dressing contains the highest total silver amount and exhibits highest release of Ag-nanoparticles and in turn the strongest cytotoxicity. The cumulative release percent of products is highly dependent on the crystal structure, silver surface coating process and binding modes with upholders. This provides an insight for rational design for further safe applications. This study establishes a systematic characterization methodology and provides comprehensive information of physicochemical properties for better understanding of risk assessment of silver-containing wound dressings.
Silver-containing wound dressings are now commonly available due to their advantage of broad-spectrum antimicrobial activities. However, few studies demonstrate their chemical composition, release kinetics and bioeffects, especially the strategies to distinguish and identify Ag-nanoparticle(Ag~0) and Ag-ion(Ag~+) forms. In this work, we have established systemic characterization and release profiles of various Ag forms in three commercial silver-containing wound dressings by combining several state-of-arts techniques, such as X-ray photoelectron spectroscope(XPS), transmission electron microscope(TEM), scanning electron microscopy with integrated energy dispersive X-ray spectroscopy(SEM-EDX), and ultrafiltration, etc. The results demonstrate that AT dressing is covered by condensed particles with size ranging from 30 to 45 nm, which mainly contains Ag~0 in face-centred cubic structure. YB and AS dressings show fibrous structures with 5~20 nm silver particles attached on the surface. YB dressing mainly contains Ag~0 and AS dressing contains both Ag~0 and Ag~+ forms. Both Ag nanoparticles and Ag ions can be found in simulated body fluids(SBF) after treating with the three dressings by the reciprocating holder method. AT dressing contains the highest total silver amount and exhibits highest release of Ag-nanoparticles and in turn the strongest cytotoxicity. The cumulative release percent of products is highly dependent on the crystal structure, silver surface coating process and binding modes with upholders. This provides an insight for rational design for further safe applications. This study establishes a systematic characterization methodology and provides comprehensive information of physicochemical properties for better understanding of risk assessment of silver-containing wound dressings.
引文
[1]Wang,L.M.;Zhang,T.L.;Li,P.Y.;Huang,W.X.;Tang,J.L.;Wang,P.Y.;Liu,J.;Yuan,Q.X.;Bai,R.;Li,B.;Zhang,K.;Zhao,Y.L.;Chen,C.Y.ACS Nano 2015,9:6532.
[2]Huo,L.L.;Chen,R.;Zhao,L.;Shi,X.F.;Bai,R.;Long,D.X.;Chen,F.;Zhao,Y.L.;Chang,Y.Z.;Chen,C.Y.Biomaterials 2015,61:307.
[2]Huo,L.L.;Chen,R.;Zhao,L.;Shi,X.F.;Bai,R.;Long,D.X.;Chen,F.;Zhao,Y.L.;Chang,Y.Z.;Chen,C.Y.Biomaterials 2015,61:307.