高效液相色谱-二极管阵列法测定人血浆中左乙拉西坦的浓度
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- 英文论文题名：Determination of Levetiracetam Concentration in serum by HPLC-DAD
- 论文作者：沈艳琳 ; 陈俊珺 ; 黄可航 ; 黄晓会
- 英文论文作者：SHEN Yan-lin ; CHEN Jun-jun ; HUANG Ke-hang ; HUANG Xiao-hui ; Department of Pharmacy ; Xinhua Hospital ; Shanghai jiaotong University School of Medicine
- 年：2017
- 作者机构：上海交通大学附属新华医院药学部;
- 论文关键词：左乙拉西坦 ; 血药浓度 ; 高效液相色谱 ; 血浆
- 英文论文关键词：Levetiracetam ; HPLC ; Drug level monitoring ; serum
- 会议召开时间：2017-05-26
- 会议录名称：2017年《中国组织化学与细胞化学杂志》临床研讨会论文集
- 语种：中文
- 分类号：R969
- 学会代码：ZHXB
- 会议名称：2017年《中国组织化学与细胞化学杂志》临床研讨会
- 会议地点：中国湖北武汉
- 主办单位：《中国组织化学与细胞化学杂志》社有限公司
- 学会名称：《中国组织化学与细胞化学杂志》社有限公司
- 页数：2
- 文件大小：330k
- 原文格式：O
- 会议级别：全国
摘要
目的:建立HPLC法测定血浆中左乙拉西坦血药浓度的方法。方法:采用甲醇沉淀蛋白,安捷伦Eclipse-XDB-C18(4.6mm×250mm,5μm)柱分离,以0.05M磷酸二氢钾缓冲液(PH=3.2):乙腈=(94:6,v/v)为流动相,二极管阵列检测器检测,紫外205nm波长检测。结果:线性范围为1.0~500μg/mL,3个不同水平添加回收率及相对标准偏差RSD(n=6)为95.5%~97.4%和1.0%~2.3%。日内精密度与日间精密度均小于5%,重现性好。方法的检出限为0.3mg·L~(-1),定量限为1.0mg·L~(-1)。结论:该方法准确,简便,精密度高,可用于左乙拉西坦血药浓度监测。
Objective:A simple HPLC method was established for the determination of Levetiracetam concentration in plasma.Methods:The Levetiracetam was extracted from the plasma samples with solvents of methanol.The extract was separated by Agilent Eclipse XDB-C18 with a mobile phase consisting of tribasic potassium phosphate buffer-methanol(94;6,v/v) and detected by diode array detector.The detection wavelength was at 205 nm.Result:The calibration curve was linear within 1.0 ~500μg/mL,The average recoveries and RSD ranged from 95.5%to 97.4%and 1.0%to 2.3%,at three spiked levels.Inter-day and intra-day RSD were 1.1%~ 1.8%and 1.0%~ 3.3%.respectively.The limit of detection(LOD) of the method was 0.3mg · L~(-1),the limit of quantity(LOQ) was 1.0mg · L~(-1).Conclusion:The proposed method appears to be a rapid,convenient and accurate method to monitor the Levetiracetam concentration in serum and can be used for its pharmacokinetic study.
Objective:A simple HPLC method was established for the determination of Levetiracetam concentration in plasma.Methods:The Levetiracetam was extracted from the plasma samples with solvents of methanol.The extract was separated by Agilent Eclipse XDB-C18 with a mobile phase consisting of tribasic potassium phosphate buffer-methanol(94;6,v/v) and detected by diode array detector.The detection wavelength was at 205 nm.Result:The calibration curve was linear within 1.0 ~500μg/mL,The average recoveries and RSD ranged from 95.5%to 97.4%and 1.0%to 2.3%,at three spiked levels.Inter-day and intra-day RSD were 1.1%~ 1.8%and 1.0%~ 3.3%.respectively.The limit of detection(LOD) of the method was 0.3mg · L~(-1),the limit of quantity(LOQ) was 1.0mg · L~(-1).Conclusion:The proposed method appears to be a rapid,convenient and accurate method to monitor the Levetiracetam concentration in serum and can be used for its pharmacokinetic study.
引文
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[6]钱进,陈凯云,罗文艳,等.高效液相色谱-二极管阵列检测器法测定老年癫痫患者左乙拉西坦的血药浓度[J].中国医院药学杂志,2013,33(16):1368-1369.
[7]王颖慧,魂敏吉,王云秀,等.高效液相色谱法测定左乙拉西坦血药浓度[J].儿科药学杂志,2010,16(4):34-36.
[2]李立敏,王宁,胡海鹏,等.左乙拉西坦治疗儿童癫痫疗效观察[J].山东医药,2016,56(2):80-81.
[3]高志杰,姜玉武.左乙拉西坦单药治疗小儿癫痫的疗效和安全性随访研究[J].中国当代儿科杂志,2008,10(6):711-714.
[4]范玲,于栋伟.高效液相色谱法在血药浓度监测中的应用进展[J].山东医药,2015,55(32):100-102.
[5]王刚,张君梅,易晓燕,等.高效液相色谱法测定癫痫患儿血清中左乙拉西坦的浓度[J].中国新药与临床杂志,2011,30(6):476-480.
[6]钱进,陈凯云,罗文艳,等.高效液相色谱-二极管阵列检测器法测定老年癫痫患者左乙拉西坦的血药浓度[J].中国医院药学杂志,2013,33(16):1368-1369.
[7]王颖慧,魂敏吉,王云秀,等.高效液相色谱法测定左乙拉西坦血药浓度[J].儿科药学杂志,2010,16(4):34-36.