UPLC-MS/MS法测定氟喹诺酮类药物可溶性粉中非法添加物利巴韦林
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- 英文论文题名：Determination of Ribavirin Illegally Added in the FQs Veterinary Medicine by UPLC-MS/MS
- 论文作者：高迎春 ; 魏秀丽 ; 张呈军 ; 薄永恒 ; 陆庆泉 ; 陈玲 ; 杨林
- 英文论文作者：GAO Yiny-chun ; WEI Xiu-li ; ZHANG Cheng-jun ; BO Yong-heng ; LU Qing-quan ; CHEN Ling ; YANG Lin ; Institute of Veterinary Drug Quality Inspection of Shandong Province ; Shandong Provincial Key Laboratory Of Quality Safety Risk Assessment for Livestock and Poultry Products
- 年：2016
- 作者机构：山东省兽药质量检验所;山东省畜产品质量安全监测与风险评估重点实验室;
- 论文关键词：利巴韦林 ; 氟喹诺酮类药物 ; 超高效液相-串联质谱法
- 英文论文关键词：Ribavirin ; FQs Veterinary Medicine ; UPLC-MS/MS
- 会议召开时间：2016-09-18
- 会议录名称：中国畜牧兽医学会动物药品学分会第五届全国会员代表大会暨2016年学术年会论文集
- 语种：中文
- 分类号：S859.79;O657.63
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物药品学分会第五届全国会员代表大会暨2016年学术年会
- 会议地点：中国四川成都
- 主办单位：中国畜牧兽医学会动物药品学分会
- 学会名称：中国畜牧兽医学会
- 页数：4
- 文件大小：456k
- 原文格式：O
- 会议级别：全国
摘要
建立了氟喹诺酮类药物可溶性粉中非法添加物利巴韦林检测的UPLC-MS/MS方法。样品经提取稀释后,采用ACQUITY UPLC~RBEH amide色谱柱为分离柱,质谱正离子扫描测定。结果显示,利巴韦林在1~50 ng/mL的范围内线性关系良好(R~2=0.9984);样品检出限为0.5 mg/g,定量限为2 mg/g。在50mg/g添加水平的回收率为90%110%,批内批间变异系数均小于10%。本方法快速、灵敏、重现性好,适用于氟喹诺酮类药物可溶性粉中非法添加利巴韦林的检测。
A method was established for determination of ribavirin added in FQs veterinary medicine by UPLC-MS/MS.The diluted ribavirin were extracted from samples and separated by ACQUITY UPLC~R BEH amide.The goals solution was analyzed by UPLC-MS/MS with positive ion mode.The results showed that the good linear range was 1 to 50 ng/mL(R~2=0.9984).The detection limit was 0.5mg/g,and the quantitation limit was 2 mg/g.The analysis of spiked 50mg/g ribavirin showed that the recoveries ranged from 90%to 110%and the relative standard deviations were below 10%.The experiment showed that the method was reliable,sensitive and reproducibility and suitable for the determination of ribavirin added in FQs veterinary medicine.
A method was established for determination of ribavirin added in FQs veterinary medicine by UPLC-MS/MS.The diluted ribavirin were extracted from samples and separated by ACQUITY UPLC~R BEH amide.The goals solution was analyzed by UPLC-MS/MS with positive ion mode.The results showed that the good linear range was 1 to 50 ng/mL(R~2=0.9984).The detection limit was 0.5mg/g,and the quantitation limit was 2 mg/g.The analysis of spiked 50mg/g ribavirin showed that the recoveries ranged from 90%to 110%and the relative standard deviations were below 10%.The experiment showed that the method was reliable,sensitive and reproducibility and suitable for the determination of ribavirin added in FQs veterinary medicine.
引文
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[2].强晓妍,朱监宝,徐敏,等.UPLC-MS/MS法测定大鼠血浆中利巴韦林及其毒代动力学参数[J].药学进展,2012,36(9):413-417.
[3].中华人民共和国农业部公告第560号[Z].2005.
[4].农业部文件农医发[2005]33号关于清查金刚烷胺等抗病毒药物的紧急通知[Z].2005.
[5].崔成富,陈创华,林海丹,等.中兽药中非法添加利巴韦林的检测方法研究[J].中国兽药杂志,2011,45(9):32-35.