光质对菊花花青素苷合成与呈色的影响
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- 英文论文题名：Effects of Different Light Qualities on Anthocyanin Biosynthesis and Coloration in Chrysanthemum(Chrysanthemum x morifolium Ramat.)
- 论文作者：李梦灵 ; 洪艳 ; 戴思兰 ; 沈如怡 ; 周义
- 英文论文作者：LI Meng-ling ; HONG Yan ; DAI Si-lan ; SHEN Ru-yi ; ZHOU Yi ; Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding ; National Engineering Research Center for Floriculture ; Beijing Laboratory of Urban and Rural Ecological Environment and College of Landscape Architecture ; Beijing Forestry University
- 年：2016
- 作者机构：花卉种质创新与分子育种北京市重点实验室国家花卉工程技术研究中心城乡生态环境北京实验室园林学院北京林业大学;
- 论文关键词：菊花 ; 花青素苷 ; 光质 ; 花色表型 ; 基因表达
- 英文论文关键词：Chrysanthemum ; Anthocyanin ; Different light qualities treatment ; Color phenotype ; Gene expression
- 会议召开时间：2016-07-19
- 会议录名称：中国观赏园艺研究进展2016
- 语种：中文
- 分类号：S682.11
- 学会代码：EGYP
- 会议名称：2016年中国观赏园艺学术研讨会
- 会议地点：中国湖南长沙
- 主办单位：中国园艺学会观赏园艺专业委员会、国家花卉工程技术研究中心
- 学会名称：中国园艺学会
- 页数：7
- 文件大小：958k
- 原文格式：O
- 会议级别：全国
摘要
光是影响植物花色最重要的环境因子之一,光质会通过影响植物花朵呈色过程中花青素苷的产生来影响花色,然而迄今关于植物花朵响应不同波长光照呈色的研究还很缺乏。菊花具有极其重要的观赏价值和商业价值,其大多数品种花朵中花青素苷的合成依赖光照。本研究以菊花品种‘丽金'紫色系为试验材料,以正常光照为对照,测定了蓝光(460nm)和红光(630nm)处理对花发育过程中舌状花的花色表型、花青素苷含量及花青素苷合成相关基因表达的影响。研究结果表明:(1)蓝光使舌状花颜色加深,而红光使花色变浅;(2)舌状花中花青素苷含量在蓝光处理下的最高值较正常光照超出225.14μg·g~(-1),而红光处理下的最高值比正常光照低3.84μg·g~(-1);(3)对花青素苷合成相关基因表达水平的分析发现:与正常光照相比,蓝光处理使CHS1、CHS2、F3H、ANS、3GT和3MT的表达量明显上调,而红光处理使CHS2、F3H和MYB4表达量明显下降。上述结果表明,不同的光质条件影响花青素苷合成相关基因的表达,使舌状花中花青素苷含量发生变化,最终影响菊花呈色。这些结果将为花青素苷响应不同光质合成的分子机理研究提供新的参考,为利用环境因子进行花色改良提供理论依据。
Light is one of the most significant environmental factors affecting flower coloration,The composition of the light spectrum has been shown to affect flower color by affecting the production of anthocyanin during the developmental state of flower.However,specific information on the coloration in flowers in response to different wavelengths of light is still scarce.Chrysanthemum has a very important ornamental and commercial value,and the synthesis of anthocyanin in most varieties of chrysanthemum is dependent on light.In the present study,chrysanthemum ' Purple Reagan' was illuminated with blue,red or white light during the development process of capitulum.Following the illumination,the color phenotype of ray floret,relative anthocyanin content and the expression of anthocyanin biosynthesis related genes during different capitulum developmental stages were measured.The main results are the followings:(1) Compared to treatment with white light,blue light treatment had significant positive effect on the coloration of the ray floret,while red light treatment inhibited the coloration;(2) The relative anthocyanin contents in ray floret increased by 225.14μg · g~(-1) under blue light treatment and decreased by 3.84μg · g~(-1)under red light treatment,compared with white light treatment(3) The process coincided with the change of the expression patterns of anthocyanin biosynthesis related genes;Compared with white light treatment,blue light treatment significantly up-regulated the expression of CHS1、 CHS2、 F3H、 ANS、 3GT and 3MT,while red light treatment down- regulated the expression of CHS2、 F3 H and MYBA.In summary,different light qualities regulated the expression of anthocyanin biosynthesis related genes,result in the change of relative anthocyanin contents in the ray floret,thus corresponding changes of the color phenotype of ray floret have occurred.These findings provide references for molecular mechanism of anthocyanin biosynthesis in response to different light qualities,and have practical significance for flower color modification by using environmental factors.
Light is one of the most significant environmental factors affecting flower coloration,The composition of the light spectrum has been shown to affect flower color by affecting the production of anthocyanin during the developmental state of flower.However,specific information on the coloration in flowers in response to different wavelengths of light is still scarce.Chrysanthemum has a very important ornamental and commercial value,and the synthesis of anthocyanin in most varieties of chrysanthemum is dependent on light.In the present study,chrysanthemum ' Purple Reagan' was illuminated with blue,red or white light during the development process of capitulum.Following the illumination,the color phenotype of ray floret,relative anthocyanin content and the expression of anthocyanin biosynthesis related genes during different capitulum developmental stages were measured.The main results are the followings:(1) Compared to treatment with white light,blue light treatment had significant positive effect on the coloration of the ray floret,while red light treatment inhibited the coloration;(2) The relative anthocyanin contents in ray floret increased by 225.14μg · g~(-1) under blue light treatment and decreased by 3.84μg · g~(-1)under red light treatment,compared with white light treatment(3) The process coincided with the change of the expression patterns of anthocyanin biosynthesis related genes;Compared with white light treatment,blue light treatment significantly up-regulated the expression of CHS1、 CHS2、 F3H、 ANS、 3GT and 3MT,while red light treatment down- regulated the expression of CHS2、 F3 H and MYBA.In summary,different light qualities regulated the expression of anthocyanin biosynthesis related genes,result in the change of relative anthocyanin contents in the ray floret,thus corresponding changes of the color phenotype of ray floret have occurred.These findings provide references for molecular mechanism of anthocyanin biosynthesis in response to different light qualities,and have practical significance for flower color modification by using environmental factors.
引文
1.陈大清.2002.不同光质和激动素对拟南芥(Arabidopsis thaliana L)幼苗光形态建成影响的研究[D].华南师范大学.
2.陈强.2009.不同LED光源对番茄果实转色过程中生理特性及果实品质的影响[D].山东农业大学.
3.胡可,韩科厅,戴思兰.2010.环境因子调控植物花青素苷合成及呈色的机理[J].植物学报,45(3):307-317.
4.李韶山,潘瑞炽.1994.蓝光对水稻幼苗叶绿体发育的影响[J].中国水稻科学,8(3):185-188.
5.孙卫,李崇晖,王亮生,等.2010.菊花不同花色品种中花青素苷代谢分析[J].园艺学报,45(3):327-336.
6.齐艳,邢燕霞,郑禾,等.2014.UV-A和UV-B提高甘蓝幼苗花青素含量以及调控基因表达分析[J].中国农业大学学报,19(2):86-94.
7.王曼,王小菁.2004.蓝光和蔗糖对拟南芥花色素苷积累和CHS基因表达的影响[J].热带亚热带植物学报,12(3):252-256.
8.王宇.2013.短波长光质诱导津田芜菁花青素合成相关基因差异表达机制研究[D].东北林业大学.
9.张泽岑,王能彬.2002.光质对茶树花青素含量的影响[J].四川农业大学学报,20(4):337-339.
10.Carreno J,Martinez A,Almela L,Fern6ndez-L6pez JA.1995.Proposal of an index for the objective evaluation of the colour of red table grapes[J].Food Research International,28(4):373-377.
11.Cominelli E,Gusmaroli G,AUegra D,Galbiatia M,Helena K,Jenkinsb G,Tonellia C.2008.Expression analysis of anthocyanin regulatory genes in response to different light qualities in Arabidopsis thaliana.[J].Journal of Plant Physiology,165(8):886-94.
12.Guo J,Han W,Wang MH.2008.Ultraviolet and environmental stresses involved in the induction and regulation of anthocyanin biosynthesis:a review[J].African Journal of Biotechnology,7,4966-4972.
13.Frohnmeyer H,Ehmann B,Kretsch T,Rocholl M,Harter K,Nagatani A,Furuya M,Batschauer A and Schafer E.1992.Differential usage of photoreceptors for chalcone synthase gene expression during plant development[J].Plant Journal,1992,2(6):899-906.
14.Hong Y,Tang XJ,Huang H,Zhang Y,Dai SL 2015.Transcriptomic analyses reveal species-specific light-induced anthocyanin biosynthesis in chrysanthemum[J].Bmc Genomics,16(1);1-18.
15.Kadomura-Ishikawa Y,Miyawaki K,Noji S,Takahashi A.2013.Phototropin 2,is involved in blue light-induced anthocyanin accumulation in Fragaria x ananassa fruits[J].Journal of Plant Research,126(6):847-57.
16.Koes R E,Spelt C E,Mol J N M.1989.The chalcone synthase multigene family of Petunia hybrida(V30):differential,light-regulated expression during flower development and UV light induction.[J].Plant Molecular Biology,12(2):213-25.
17.Meng X,Xing T and Wang X.2004.The role of light in the regulation of anthocyanin accumulation in Gerbera hybrida[J].Plant Growth Regulation,44(3):243-250.
18.Tanaka Y,Ohmiya A.2008.Seeing is believing:engineering anthocyanin and carotenoid biosynthetic pathways[J].Current Opinion in Biotechnology,19(2):190-7.
19.Ubi B E,Honda C,Bessho H,Kondod S,Wadac M,Kobayashia S,Moriguchia T.2006.Expression analysis of anthocyanin biosynthetic genes in apple skin;Effect of UV-B and temperature[J].Plant Science,170(3):571-578.
20.Weiss D.2000.Regulation of flower pigmentation and growth:Multiple signaling pathways control anthocyanin synthesis in expanding petals[J].Physiologia Plantarum,110(2):152-157.
21.Zhao D,Tao J.2015.Recent advances on the development and regulation of flower color in ornamental plants[J].Frontiers in Plant Science,6;261.
22.Zhou B,Li Y,Xu Z,Yan H,Homma S,Kawabata S.2007.Ultraviolet A-specific induction of anthocyanin biosynthesis in the swollen hypocotyls of turnip(Brassica rapa)[J].Journal of Experimental Botany,58:1771-1781.
23.Zoratti L,Sarala M,Carvalho E,Karppinen K,Martens S,Giongo L,Haggman H and Jaakola L 2014.Monochromatic light increases anthocyanin content during fruit development in bilberry[J].Bmc Plant Biology,14(1):1-10.
2.陈强.2009.不同LED光源对番茄果实转色过程中生理特性及果实品质的影响[D].山东农业大学.
3.胡可,韩科厅,戴思兰.2010.环境因子调控植物花青素苷合成及呈色的机理[J].植物学报,45(3):307-317.
4.李韶山,潘瑞炽.1994.蓝光对水稻幼苗叶绿体发育的影响[J].中国水稻科学,8(3):185-188.
5.孙卫,李崇晖,王亮生,等.2010.菊花不同花色品种中花青素苷代谢分析[J].园艺学报,45(3):327-336.
6.齐艳,邢燕霞,郑禾,等.2014.UV-A和UV-B提高甘蓝幼苗花青素含量以及调控基因表达分析[J].中国农业大学学报,19(2):86-94.
7.王曼,王小菁.2004.蓝光和蔗糖对拟南芥花色素苷积累和CHS基因表达的影响[J].热带亚热带植物学报,12(3):252-256.
8.王宇.2013.短波长光质诱导津田芜菁花青素合成相关基因差异表达机制研究[D].东北林业大学.
9.张泽岑,王能彬.2002.光质对茶树花青素含量的影响[J].四川农业大学学报,20(4):337-339.
10.Carreno J,Martinez A,Almela L,Fern6ndez-L6pez JA.1995.Proposal of an index for the objective evaluation of the colour of red table grapes[J].Food Research International,28(4):373-377.
11.Cominelli E,Gusmaroli G,AUegra D,Galbiatia M,Helena K,Jenkinsb G,Tonellia C.2008.Expression analysis of anthocyanin regulatory genes in response to different light qualities in Arabidopsis thaliana.[J].Journal of Plant Physiology,165(8):886-94.
12.Guo J,Han W,Wang MH.2008.Ultraviolet and environmental stresses involved in the induction and regulation of anthocyanin biosynthesis:a review[J].African Journal of Biotechnology,7,4966-4972.
13.Frohnmeyer H,Ehmann B,Kretsch T,Rocholl M,Harter K,Nagatani A,Furuya M,Batschauer A and Schafer E.1992.Differential usage of photoreceptors for chalcone synthase gene expression during plant development[J].Plant Journal,1992,2(6):899-906.
14.Hong Y,Tang XJ,Huang H,Zhang Y,Dai SL 2015.Transcriptomic analyses reveal species-specific light-induced anthocyanin biosynthesis in chrysanthemum[J].Bmc Genomics,16(1);1-18.
15.Kadomura-Ishikawa Y,Miyawaki K,Noji S,Takahashi A.2013.Phototropin 2,is involved in blue light-induced anthocyanin accumulation in Fragaria x ananassa fruits[J].Journal of Plant Research,126(6):847-57.
16.Koes R E,Spelt C E,Mol J N M.1989.The chalcone synthase multigene family of Petunia hybrida(V30):differential,light-regulated expression during flower development and UV light induction.[J].Plant Molecular Biology,12(2):213-25.
17.Meng X,Xing T and Wang X.2004.The role of light in the regulation of anthocyanin accumulation in Gerbera hybrida[J].Plant Growth Regulation,44(3):243-250.
18.Tanaka Y,Ohmiya A.2008.Seeing is believing:engineering anthocyanin and carotenoid biosynthetic pathways[J].Current Opinion in Biotechnology,19(2):190-7.
19.Ubi B E,Honda C,Bessho H,Kondod S,Wadac M,Kobayashia S,Moriguchia T.2006.Expression analysis of anthocyanin biosynthetic genes in apple skin;Effect of UV-B and temperature[J].Plant Science,170(3):571-578.
20.Weiss D.2000.Regulation of flower pigmentation and growth:Multiple signaling pathways control anthocyanin synthesis in expanding petals[J].Physiologia Plantarum,110(2):152-157.
21.Zhao D,Tao J.2015.Recent advances on the development and regulation of flower color in ornamental plants[J].Frontiers in Plant Science,6;261.
22.Zhou B,Li Y,Xu Z,Yan H,Homma S,Kawabata S.2007.Ultraviolet A-specific induction of anthocyanin biosynthesis in the swollen hypocotyls of turnip(Brassica rapa)[J].Journal of Experimental Botany,58:1771-1781.
23.Zoratti L,Sarala M,Carvalho E,Karppinen K,Martens S,Giongo L,Haggman H and Jaakola L 2014.Monochromatic light increases anthocyanin content during fruit development in bilberry[J].Bmc Plant Biology,14(1):1-10.