酸性电解水对食源性致病菌的作用位点研究
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- 英文论文题名：Determination of injury sites in foodborne pathogens caused by acidic electrolyzed water
- 论文作者：巴永兵 ; 赵莉 ; 刘海泉 ; 潘迎捷 ; 赵勇
- 英文论文作者：Ba Yongbing ; Zhao Li ; Liu Haiquan ; Pan Yingjie ; Zhao Yong ; College of Food Science and Technology ; Shanghai Ocean University ; Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation ; Laboratory of Quality & Safety Risk Assessment for Aquatic Product on Storage and Preservation(Shanghai) ; Ministry of Agriculture
- 年：2016
- 作者机构：上海海洋大学食品学院;上海水产品加工及贮藏工程技术研究中心;农业部水产品贮藏保鲜质量安全风险评估实验室(上海);
- 论文关键词：酸性电解水 ; 副溶血性弧菌 ; 杀菌机制 ; 新陈代谢抑制剂
- 英文论文关键词：acidic electrolyzed water ; vibrio parahemolyticus ; sterilization mechanism ; metabolic inhibitors
- 会议召开时间：2016-11-09
- 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
- 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
- 语种：中文
- 分类号：TS201.3
- 学会代码：ZGSP
- 会议名称：中国食品科学技术学会第十三届年会
- 会议地点：中国北京
- 主办单位：中国食品科学技术学会
- 学会名称：中国食品科学技术学会
- 页数：2
- 文件大小：112k
- 原文格式：O
- 会议级别：全国
摘要
[目的]酸性电解水是一种高效的杀菌剂,近年来在食品产业的杀菌保鲜上得到广泛关注。本研究主要探究了酸性电解水对食源性致病菌的作用位点。[方法]选取副溶血性弧菌ATCC17802为本次研究对象,利用非选择性培养基可以修复受损伤菌,而受损伤菌在选择性培养基中无法生长的原理进行靶向实验。应用四种作用原理已知的新陈代谢抑制剂探究酸性电解水对食源性致病菌的作用位点为哪一种(四种新陈代谢抑制剂,氯霉素的作用位点是核糖体蛋白,萘啶酸的作用位点是DNA,盘尼西林为细胞壁,利福平的作用位点是RNA聚合酶。[结果]根据预实验结果,将0.1%NaCl电解制得的电解水稀释十倍后选为本次实验的酸性电解水浓度,选取的抗生素临界浓度分别为:0.25μg/mL的氯霉素、0.125μg/mL的萘啶酸、4μg/mL的盘尼西林、0.01875μg/mL的利福平(此临界浓度为抗生素刚好对细菌不产生作用的最大浓度)。电解水处理后结果发现,氯霉素、盘尼西林和利福平对副溶血性弧菌的修复作用有显著性的抑制作用(p<0.05),而萘啶酸则没有显著性的抑制(p>0.05)。[结论]结果表明酸性电解水主要破坏食源性致病菌的细胞壁、核糖体和RNA聚合酶。
[Objective]Acidic electrolyzed water(AEW),an effective bactericidal agent,has gained interest as a newly developed disinfectant in the food industry.In this study,injury sites in pathogenic-bacteria caused by acidic electrolyzed water were investigated.[Methods]Overlay method was used to enumerate injured cells of Vibrio parahemolyticus ATCC17802.The principle is that nonselective medium can repair injured cells,instead,injured cells can not growth in selective medium.Four types of metabolic inhibitors,of which primary synthesis inhibitions are well-known,were employed in order to specify the character of the injury in the target microorganism,(four metabolic inhibitors,chloramphenicol targets Ribosomal protein,nalidixic acid targets DNA,Penicillin G targets cell wall,and rifampin targets RNA polymerase).[Results]0.1%NaCl concentration to electrolysis in 10-fold dilution and the concentrations of metabolic inhibitors,chloramphenicol(0.25μg/mL),nalidixic acid(0.125μg/mL),penicillin G(4μg/mL),and rifampin(0.01875μg/mL),were chosen after preliminary experiments were performed.After AEW treatment,significant(p < 0.05) inhibition of repair was detected in the presence of chloramphenicol.Similar results were found in the presence of penicillin G or rifampin.Nalidixic acid did not affect the viability of AEW-treated cells.[Conclusion]There results showed that the sites of cellular damage of AEW on pathogenic bacteria might be related to damage to cellular wall,ribosomal,and inhibit RNA synthesis.
[Objective]Acidic electrolyzed water(AEW),an effective bactericidal agent,has gained interest as a newly developed disinfectant in the food industry.In this study,injury sites in pathogenic-bacteria caused by acidic electrolyzed water were investigated.[Methods]Overlay method was used to enumerate injured cells of Vibrio parahemolyticus ATCC17802.The principle is that nonselective medium can repair injured cells,instead,injured cells can not growth in selective medium.Four types of metabolic inhibitors,of which primary synthesis inhibitions are well-known,were employed in order to specify the character of the injury in the target microorganism,(four metabolic inhibitors,chloramphenicol targets Ribosomal protein,nalidixic acid targets DNA,Penicillin G targets cell wall,and rifampin targets RNA polymerase).[Results]0.1%NaCl concentration to electrolysis in 10-fold dilution and the concentrations of metabolic inhibitors,chloramphenicol(0.25μg/mL),nalidixic acid(0.125μg/mL),penicillin G(4μg/mL),and rifampin(0.01875μg/mL),were chosen after preliminary experiments were performed.After AEW treatment,significant(p < 0.05) inhibition of repair was detected in the presence of chloramphenicol.Similar results were found in the presence of penicillin G or rifampin.Nalidixic acid did not affect the viability of AEW-treated cells.[Conclusion]There results showed that the sites of cellular damage of AEW on pathogenic bacteria might be related to damage to cellular wall,ribosomal,and inhibit RNA synthesis.
引文