‘美人'梅不同扦插时间嫩枝扦插叶片内相关生理生化变化
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- 英文论文题名：Related Physiological and Biochemical Changes in Blade during Rooting of Prunus mume ‘Meiren’ in Different Cutting Date
- 论文作者：董然然 ; 陈瑞丹
- 英文论文作者：DONG Ran-ran ; CHEN Rui-dan ; Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding ; National Engineering Research Center for Floriculture ; Beijing Laboratory of Urban and Rural Ecological Environment and College of Landscape Architecture ; Beijing Forestry University
- 年：2016
- 作者机构：花卉种质创新与分子育种北京市重点实验室国家花卉工程技术研究中心城乡生态环境北京实验室园林学院北京林业大学;
- 论文关键词：‘美人’梅 ; 嫩枝扦插 ; 叶片 ; 营养物质 ; 酶活性
- 英文论文关键词：Prunus mume ‘Meiren’ ; Softwood cutting ; Blade ; Nutrients ; Oxidases
- 会议召开时间：2016-07-19
- 会议录名称：中国观赏园艺研究进展2016
- 语种：中文
- 分类号：S685.17
- 学会代码：EGYP
- 会议名称：2016年中国观赏园艺学术研讨会
- 会议地点：中国湖南长沙
- 主办单位：中国园艺学会观赏园艺专业委员会、国家花卉工程技术研究中心
- 学会名称：中国园艺学会
- 页数：6
- 文件大小：1021k
- 原文格式：O
- 会议级别：全国
摘要
为研究不同扦插时间‘美人'梅扦插后插穗叶片的相关生理生化变化,以‘美人'梅半木质化嫩枝为材料,分别在5月份和8月份采用2500 mg·L~(-1)IBA处理插穗,测定扦插后不同时期叶片内可溶性糖、可溶性蛋白、淀粉含量和过氧化物酶(POD)、多酚氧化酶(PPO)、吲哚乙酸氧化酶(IAAO)活性的变化。结果表明:①母株叶片内的IAAO活性较高可能是导致‘美人'梅生根率季节性差异的原因。②生根过程中,可溶性糖含量均呈现"升高-下降-升高"的变化趋势,而淀粉含量均呈现先下降后升高的现象;8月份可溶性糖及淀粉含量峰值点相比于5月份均存在滞后现象,可溶性蛋白含量变化趋势相比与5月份不明显。PPO活性在5月份与8月份呈现相反的规律;8月份,POD活性变化趋势不明显,且其活性较低,IAAO活性较高,可能是导致其生根率较低的原因。研究表明:母株叶片内的IAAO活性以及扦插生根过程中,叶片内的3种营养物质含量和3种氧化酶的活性及其相关变化趋势与扦插生根密切相关。5月份,母株叶片内的IAAO活性较低,生根过程中叶片内的可溶性糖及淀粉含量峰值提前,POD活性出现两个明显的峰值,LAAO活性较低,可能对生根有利。
The objective was to study the related physiological and biochemical changes in blade during rooting of Prunusmume‘Meiren' in different cutting date.Contents of soluble sugar,starch,soluble protein and activities of POD,PPO,IAAOin phloem at various rooting phase were measuredin asimplerandom replicationexperiment.The semi-hard wood stems were collected and treated with IBA at a concentration of 2500 mg·L~(-1)with water dipping as control in May and August respectively.The result showed:①The high IAAO activity in matemalmay cause cutting date effect,②uring the rooting process,the soluble sugar content increased at first and then decreased,and it increased again at last,while the starch content fall before increased.The peak of the content of soluble sugar and starch during the cutting in August compared with that in May existed hysteresis and the changing trend of soluble content was not obvious.During the rooting process,the activity of PPO changed on the contrary in May and August,at the same time the POD activity was lower and IAAO activity was higher,and the variation trend of PPO activity was unconspicuous,that might be why the rooting percentage in August was so low.Our results suggested that the IAAO activity in maternal and the threenutrients and three oxidases during cutting were closely related to rooting.In May,the IAAO activity in maternal was lower and in rooting process the content peaks of soluble sugar and starch were in advance and POD activity appeared two obvious peaks while IAAO activity was lower,which may be favored for rooting.
The objective was to study the related physiological and biochemical changes in blade during rooting of Prunusmume‘Meiren' in different cutting date.Contents of soluble sugar,starch,soluble protein and activities of POD,PPO,IAAOin phloem at various rooting phase were measuredin asimplerandom replicationexperiment.The semi-hard wood stems were collected and treated with IBA at a concentration of 2500 mg·L~(-1)with water dipping as control in May and August respectively.The result showed:①The high IAAO activity in matemalmay cause cutting date effect,②uring the rooting process,the soluble sugar content increased at first and then decreased,and it increased again at last,while the starch content fall before increased.The peak of the content of soluble sugar and starch during the cutting in August compared with that in May existed hysteresis and the changing trend of soluble content was not obvious.During the rooting process,the activity of PPO changed on the contrary in May and August,at the same time the POD activity was lower and IAAO activity was higher,and the variation trend of PPO activity was unconspicuous,that might be why the rooting percentage in August was so low.Our results suggested that the IAAO activity in maternal and the threenutrients and three oxidases during cutting were closely related to rooting.In May,the IAAO activity in maternal was lower and in rooting process the content peaks of soluble sugar and starch were in advance and POD activity appeared two obvious peaks while IAAO activity was lower,which may be favored for rooting.
引文
1.陈俊愉.1988.梅花与园林[M].北京:北京科学技术出版社.20.
2.陈俊愉.1989.中国梅花品种图志[M].北京:中国林业出版社,28-30.
3.陈俊愉.2001.中国花卉品种分类学[M].北京:中国林业出版社,88-89.
4.陈有民.1990.园林树木学[M].北京:中国林业出版社,438-440.
5.董然然,陈瑞丹.2016.‘美人'梅不同扦插时间嫩枝扦插生根特性及相关生理生化变化[J].浙江农业学报,(2016年9-10月份见刊)
6.杜蕊.2012.矮生彩色针叶树扦插繁殖年龄效应及栽培技术研究[D].北京林业大学.
7.GasparT,Kevers C,Hausman J F,Berthon J Y,Ripetti V.1992.Practical uses of peroxidase activity as a predictive marker of rooting performance of micropropagated shoots[J].Agronomy for Sustainable Development,10(12):757-765.
8.高辉远,孟庆伟.2011.植物生理学[M].北京:中国农业出版社,196.
9.郭英超.2007.兴安园柏扦插繁殖技术及插穗生根生理基础的研究[D].河北农业大学.
10.Haissig B E.1974.Influence of auxins and auxin synergists on adventitious primordium initiation and development[J].New Zealand Journal Forestry Science,4;311-323.
11.扈红军,曹帮华,尹伟伦,等.2007.不同处理对欧榛硬枝扦插生根的影响及生根过程中相关氧化酶活性的变化[J].林业科学,43(12):70-75.
12.黄卓烈,李明,谭绍满,等.2003.吲哚丁酸对桉树插条多酚氧化酶的影响及其与生根的关系[J].广西植物,23(1):77-82.
13.李合生.1999.植物生理生化实验原理和技术[M].北京:高等教育出版社,164,185,195.
14.李明,黄卓烈,谭绍满,等.2011.难易生根桉树的过氧化物酶活性及其同工酶多型性比较研究[J].林业科学研究,14(2):131-140.
15.李振坚,陈瑞丹,李庆卫,等.2009.生长素和基质对梅花嫩枝扦插生根的影响[J].林业科学研究,22(1):120-123.
16.刘玉民,刘亚敏,马明,等.2010.马尾松扦插生根过程相关生理生化分析[J].林业科学,46(9):28-33.
17.刘玉艳,于凤鸣,于娟.2003.对含笑扦插生根影响初探[J].河北农业大学学报,26(2):25-29.
18.马振华,赵忠,张晓鹏,等.2007.四倍体刺槐扦插生根过程中氧化酶活性的变化[J].西北农林科技大学学报(自然科学版),35(7):85-89.
19.Murai Y,Harada H,Mochioka R,Ogata T,Shiozaki S,Horiuchi S,Mukai H,Takagi T.1999.Relationships between rooting in softwood cuttings of mume(Primus mume Sieb.et Zucc.)and sorbitol in shoots[J].Journal Japanese Society Horticulture Science,3(68):648-654.
20.Ouyama N,Morishita Y.The principle and technology of cutting[M].Beijing:Chinese Forestry Publishing House,1988:8-10.(in Chinese)
21.Satisha J,Raveendran P,Rokade N D.2008.Changes in polyphenol oxidase activity during rooting of hardwood cuttings in three grape rootstocks under Indian conditions[J].South African Journal of Enology,2(29):94-97.
22.宋金耀,宋刚,李辉,等.2010.几种园艺植物扦插生根过程中生化指标的变化[J].江苏农业科学,(3):211-214.
23.王小玲,高柱,余发新,等.2010.观赏羽扇豆离体培养生根关联酶活性及可溶性蛋白含量变化规律研究[J].云南省农业大学学报,25(6):835-839.
24.王小玲,赵忠,权金娥,等.2011.外源激素对四倍体刺槐硬枝扦插生根及其关联酶活性的影响[J].西北植物学报,21(1):116-112.
25.魏海蓉,陈新,宗晓娟,等.2013.甜樱桃矮化砧‘吉塞拉6号'扦插过程中氧化酶活性和碳氮含量变化[J].林业科学,49(9):172-177.
26.闫绍鹏,武晓东,王秋玉,等.2011.欧美山杨杂种嫩枝微扦插生根相关氧化酶活性变化及繁殖技术[J].东北林业大学学报,39(11):5-7.
27.原牡丹,候智霞,翟明普,等.2008.分解代谢相关酶(IAAO、PPO)的研究进展[J].中国农学通报,24(8):88-92.
28.张行言,王其超,包满珠.1998.梅花[M].上海:上海科学技术出版社,101-110.
29.赵云龙,陈训,李朝婵.2013.糙叶杜鹃扦插生根过程中生理生化分析[J].林业科学,49(6):45-51.
30.张志良,瞿伟菁,李小芳.2009.植物生理学实验指导[M].北京:高等教育出版社,155.
31.郑晨.2011.南京椴扦插繁殖与生根机理研究[D].南京林业大学.
2.陈俊愉.1989.中国梅花品种图志[M].北京:中国林业出版社,28-30.
3.陈俊愉.2001.中国花卉品种分类学[M].北京:中国林业出版社,88-89.
4.陈有民.1990.园林树木学[M].北京:中国林业出版社,438-440.
5.董然然,陈瑞丹.2016.‘美人'梅不同扦插时间嫩枝扦插生根特性及相关生理生化变化[J].浙江农业学报,(2016年9-10月份见刊)
6.杜蕊.2012.矮生彩色针叶树扦插繁殖年龄效应及栽培技术研究[D].北京林业大学.
7.GasparT,Kevers C,Hausman J F,Berthon J Y,Ripetti V.1992.Practical uses of peroxidase activity as a predictive marker of rooting performance of micropropagated shoots[J].Agronomy for Sustainable Development,10(12):757-765.
8.高辉远,孟庆伟.2011.植物生理学[M].北京:中国农业出版社,196.
9.郭英超.2007.兴安园柏扦插繁殖技术及插穗生根生理基础的研究[D].河北农业大学.
10.Haissig B E.1974.Influence of auxins and auxin synergists on adventitious primordium initiation and development[J].New Zealand Journal Forestry Science,4;311-323.
11.扈红军,曹帮华,尹伟伦,等.2007.不同处理对欧榛硬枝扦插生根的影响及生根过程中相关氧化酶活性的变化[J].林业科学,43(12):70-75.
12.黄卓烈,李明,谭绍满,等.2003.吲哚丁酸对桉树插条多酚氧化酶的影响及其与生根的关系[J].广西植物,23(1):77-82.
13.李合生.1999.植物生理生化实验原理和技术[M].北京:高等教育出版社,164,185,195.
14.李明,黄卓烈,谭绍满,等.2011.难易生根桉树的过氧化物酶活性及其同工酶多型性比较研究[J].林业科学研究,14(2):131-140.
15.李振坚,陈瑞丹,李庆卫,等.2009.生长素和基质对梅花嫩枝扦插生根的影响[J].林业科学研究,22(1):120-123.
16.刘玉民,刘亚敏,马明,等.2010.马尾松扦插生根过程相关生理生化分析[J].林业科学,46(9):28-33.
17.刘玉艳,于凤鸣,于娟.2003.对含笑扦插生根影响初探[J].河北农业大学学报,26(2):25-29.
18.马振华,赵忠,张晓鹏,等.2007.四倍体刺槐扦插生根过程中氧化酶活性的变化[J].西北农林科技大学学报(自然科学版),35(7):85-89.
19.Murai Y,Harada H,Mochioka R,Ogata T,Shiozaki S,Horiuchi S,Mukai H,Takagi T.1999.Relationships between rooting in softwood cuttings of mume(Primus mume Sieb.et Zucc.)and sorbitol in shoots[J].Journal Japanese Society Horticulture Science,3(68):648-654.
20.Ouyama N,Morishita Y.The principle and technology of cutting[M].Beijing:Chinese Forestry Publishing House,1988:8-10.(in Chinese)
21.Satisha J,Raveendran P,Rokade N D.2008.Changes in polyphenol oxidase activity during rooting of hardwood cuttings in three grape rootstocks under Indian conditions[J].South African Journal of Enology,2(29):94-97.
22.宋金耀,宋刚,李辉,等.2010.几种园艺植物扦插生根过程中生化指标的变化[J].江苏农业科学,(3):211-214.
23.王小玲,高柱,余发新,等.2010.观赏羽扇豆离体培养生根关联酶活性及可溶性蛋白含量变化规律研究[J].云南省农业大学学报,25(6):835-839.
24.王小玲,赵忠,权金娥,等.2011.外源激素对四倍体刺槐硬枝扦插生根及其关联酶活性的影响[J].西北植物学报,21(1):116-112.
25.魏海蓉,陈新,宗晓娟,等.2013.甜樱桃矮化砧‘吉塞拉6号'扦插过程中氧化酶活性和碳氮含量变化[J].林业科学,49(9):172-177.
26.闫绍鹏,武晓东,王秋玉,等.2011.欧美山杨杂种嫩枝微扦插生根相关氧化酶活性变化及繁殖技术[J].东北林业大学学报,39(11):5-7.
27.原牡丹,候智霞,翟明普,等.2008.分解代谢相关酶(IAAO、PPO)的研究进展[J].中国农学通报,24(8):88-92.
28.张行言,王其超,包满珠.1998.梅花[M].上海:上海科学技术出版社,101-110.
29.赵云龙,陈训,李朝婵.2013.糙叶杜鹃扦插生根过程中生理生化分析[J].林业科学,49(6):45-51.
30.张志良,瞿伟菁,李小芳.2009.植物生理学实验指导[M].北京:高等教育出版社,155.
31.郑晨.2011.南京椴扦插繁殖与生根机理研究[D].南京林业大学.