摘要
<正>魔芋葡甘聚糖(KGM)是一种水溶性多糖,具有良好的生物相容性,具备作为理想生物材料的优良特性。本论文以魔芋粗粉为原料,精制提纯制得魔芋精粉,采用悬浮搅拌法经过化学交联制备出KGM微球,对KGM微球微球用二乙胺基盐酸(DEAE-HCl)进行修饰,制备了细胞培养微载体。利用正交实验法对影响微载体上细胞生长密度的微载体粒径、培基密度、魔芋浓度、交
引文
[1]Sun X,Zhang Y,Tan W,Zhou Y,Hua P.Attachment kinetics of Vero cells onto CT-3 microcarriers.Journal of bioscience and bioengineering,2000,90:32-36
[2]Rodrigues M E,Costa A R,Fernandes P,Henriques M,Cunnah P.Evaluation of macroporous and microporous carriers for CKO-K1 cell growth and monoclonal antibody production.J Microbiol Biotechn,2013,23:1308-13