复方中药萃液对卷烟的生物学减害作用研究
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- 英文论文题名：Effect of Chinese Herbal Compound Extract on Reducing Biological Harmful in Cigarette
- 论文作者：吴彦 ; 田兆福 ; 孟冬玲 ; 杜树山 ; 王萍娟 ; 朱茂祥 ; 白家峰 ; 黄东业
- 英文论文作者：WU Yan ; TIAN Zhaofu ; Meng Dongling ; DU Shushan ; WANG Pingjuan ; ZHU Maoxiang ; BAI Jiafeng ; HUANG Dongye ; Technical Center ; China tobacco Guangxi Industrial Co. ; Ltd ; College of Resources Science & Technology ; Beijing Normal University ; Institute of Radiation Medicine ; Academy of Military Medical Sciences
- 年：2016
- 作者机构：广西中烟工业有限责任公司技术中心;北京师范大学资源学院;军事医学科学院放射与辐射医学研究所;
- 论文关键词：复方中药萃液 ; 卷烟烟气 ; 细胞保护 ; 镇咳祛痰 ; 体外毒性
- 英文论文关键词：Chinese herbal compound extract ; Cigarette smoke ; cytoprotection ; antitussive expectorant ; cytotoxicity in vitro
- 会议召开时间：2016-12-01
- 会议录名称：中国烟草学会2016年度优秀论文汇编——烟草工业主题
- 语种：中文
- 分类号：R285
- 学会代码：ZGYG
- 学会名称：中国烟草学会
- 页数：7
- 文件大小：900k
- 原文格式：D
摘要
目的:研究复方中药萃液对卷烟的生物学减害作用。方法:分别检测卷烟烟气对CHO细胞的细胞毒性、鼠伤寒沙门氏菌诱变性和体外细胞微核率、BEP2D细胞增殖、及对动物卷烟烟气暴露30天后外周血细胞改变、致咳反应、泌痰反应的影响,并观察复方中药萃液对这些影响的作用。结果:复方中药萃液添加后不增加卷烟烟气对鼠伤寒沙门氏菌诱变性及对体外细胞微核的诱导作用,并能抑制卷烟烟气对CHO细胞的细胞毒作用;复方中药萃液添加后对卷烟烟气对人呼吸道上皮细胞的损伤具有良好的保护作用;可以逆转在动物烟气暴露30天气引起的血红蛋白下降,血小板数量增加状况(P<0.05);逆转卷烟烟气引起的动物咳嗽潜伏期降低、咳嗽次数增加、酚红排泌量降低等症状(p<0.05)。结论:复方中药萃液不增加卷烟烟气的体外毒性且有一定减轻毒性的作用或趋势;可以通过减轻吸烟引起的咳嗽、痰液粘稠咳出困难、外周血液学改变等症状,及对呼吸道上皮细胞的直接的保护作用减轻卷烟烟气对机体损伤。
Objective: To study the effect of Chinese herbal compound extract on Reducing Biological Harmful in Cigarette. Method: Detect the effect of Cigarette Mainstream smoke on cytotoxicity toward chinese hamster ovarian cells(CHO cells), thesalmonella mutagenicitytest, micronucleus test, proliferation of BEP2 D cells in vitro, and on peripheral blood cells, ammonia induced cough, phenolsulfanphthalein excretion test in vivo. Observe intervention effects of Chinese herb compound on all these test. Result: Chinese herbal compound extract does not increase thesalmonella mutagenicitytest and micronucleus frequency and could reduce the cytotoxicity toward CHO cells induced by Cigarette smoke. Chinese herbal compound extract could reduce the injury of human respiratory epithelial cells and reverse the changes of peripheral blood cells induced by Cigarette smoke. Chinese herbal compound extract could reverse the shorten of latent period of cough and the increasing of times of cough(p<0.05),and reverse the decrease of excretion of phenolsulfonphthalein(p<0.05) caused by cigarette smoke in mice. Conclusion: Chinese herbal compound extract doesn't increase the in vitro cyto-toxicity of cigarette smoke and even shows reducing effect or tendency, Chinese herbal compound extract could reduce the injury of cigarette smoke by reducing the cough, thick sputum and change of peripheral blood cells induced by smoking, and though directly protective effect on respiratory epithelial cells.
Objective: To study the effect of Chinese herbal compound extract on Reducing Biological Harmful in Cigarette. Method: Detect the effect of Cigarette Mainstream smoke on cytotoxicity toward chinese hamster ovarian cells(CHO cells), thesalmonella mutagenicitytest, micronucleus test, proliferation of BEP2 D cells in vitro, and on peripheral blood cells, ammonia induced cough, phenolsulfanphthalein excretion test in vivo. Observe intervention effects of Chinese herb compound on all these test. Result: Chinese herbal compound extract does not increase thesalmonella mutagenicitytest and micronucleus frequency and could reduce the cytotoxicity toward CHO cells induced by Cigarette smoke. Chinese herbal compound extract could reduce the injury of human respiratory epithelial cells and reverse the changes of peripheral blood cells induced by Cigarette smoke. Chinese herbal compound extract could reverse the shorten of latent period of cough and the increasing of times of cough(p<0.05),and reverse the decrease of excretion of phenolsulfonphthalein(p<0.05) caused by cigarette smoke in mice. Conclusion: Chinese herbal compound extract doesn't increase the in vitro cyto-toxicity of cigarette smoke and even shows reducing effect or tendency, Chinese herbal compound extract could reduce the injury of cigarette smoke by reducing the cough, thick sputum and change of peripheral blood cells induced by smoking, and though directly protective effect on respiratory epithelial cells.
引文
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2 .吴彦,杨鸿,刘红,等.中药组分清除烟气有害物质及降低细胞毒性的研究[J].中国中药杂志.2011,36(22):3184-3188.
3 .杨鸿,吴彦,马琰岩,等.清除致癌物多环芳烃的中药组分复方配伍研究[J].中国实验方剂学杂志.2012,18(8):228-231.
4 .杨鸿,吴彦,马琰岩,等.具抗氧化活性的中药有效组分的配伍研究[J].中国中药杂志.2012,37(12):1826-1829.
5 .GB/T 16450-2004,常规分析用吸烟机定义的标准条件[S].北京:中国标准出版社,2004.
6 .谢剑平,朱茂祥,杨陟华,等.评价卷烟烟气毒性的小鼠体内微核分析检测方法[P].中国专利:200810049451.9,2008-04-01.
7 .朱茂祥,郑劲林,杨陟华,等.动物气体染毒装置[P].中国专利:201110406975.0,2011-12-08.