5/6肾切除大鼠慢性肾功能衰竭经典模型的建立
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- 英文论文题名：5/6 Nephrectomy in Rats with Chronic Renal Failure and Classical Model
- 论文作者：孙梅艳
- 英文论文作者：Sun Meiyan ; Chuxiong Medical College
- 年：2017
- 作者机构：楚雄医药高等专科学校;
- 论文关键词：5/6肾切除/慢性肾功能衰竭 ; 血尿素氮(BUN) ; 血肌酐(SCR)
- 英文论文关键词：5/6 Nephrectomy/chronic Renal Failure ; Blood Urea Nitrogen(BUN) ; Serum Creatinine(SCR)
- 会议召开时间：2017-05-26
- 会议录名称：2017年《中国组织化学与细胞化学杂志》临床研讨会论文集
- 语种：中文
- 分类号：R-332;R692.5
- 学会代码：ZHXB
- 会议名称：2017年《中国组织化学与细胞化学杂志》临床研讨会
- 会议地点：中国湖北武汉
- 主办单位：《中国组织化学与细胞化学杂志》社有限公司
- 学会名称：《中国组织化学与细胞化学杂志》社有限公司
- 页数：1
- 文件大小：392k
- 原文格式：O
- 会议级别：全国
摘要
目的:通过动物实验研究探讨寻找建立接近临床实际,适用广泛、统一、简便、稳定的CRF动物模型,对研究CRF的发病机理,探讨组织形态的变化与生化指标及临床表现的相互关系,为深入到细胞和分子水平研究CRF,筛选有效药物,阐明疗效机制,提供符合人类自然发病病理表现经典动物模型。方法:采用5/6肾切除建立CRF大鼠模型,分为模型组、未手术组(空白组)、假手术组(正常对照组)。观察各组大鼠血清尿素氮(BUN)、肌酐(Scr)、肾组织HE、masson染色病理切片观察。结果:5/6肾切除大鼠血清BUN(P<0.01)、Scr(P<0.01);病理切片残余肾脏组织纤维化存在。结论:5/6肾切除大鼠慢性肾衰竭BUN(P<0.01)、Scr(P<0.01),病理切片示残肾纤维化存在,符合人类慢性肾衰竭肾脏的病理学特征,证明造模成功。
Objective:through the animal experiment research for establishing close to clinical practice,and is used widely,unified,simple and stable animal classic model of CRF.Methods:5/6 nephrectomy is adopted to establish the CRF rats model,were divided into model group,positive control group,at the same time a control group(normal control group).Observe each group rats serum urea nitrogen(BUN),creatinine(Scr),kidney tissues HE and masson staining pathological observation.Results:5/6 nephrectomy in rats serum BUN(P < 0.01),Scr(P < 0.01);Residual kidney pathological tissue fibrosis.Conclusion:5/6 nephrectomy in rats with chronic renal failure BUN(P < 0.01),the Scr(P < 0.01),pathological section residual renal fibrosis,proved that building success.
Objective:through the animal experiment research for establishing close to clinical practice,and is used widely,unified,simple and stable animal classic model of CRF.Methods:5/6 nephrectomy is adopted to establish the CRF rats model,were divided into model group,positive control group,at the same time a control group(normal control group).Observe each group rats serum urea nitrogen(BUN),creatinine(Scr),kidney tissues HE and masson staining pathological observation.Results:5/6 nephrectomy in rats serum BUN(P < 0.01),Scr(P < 0.01);Residual kidney pathological tissue fibrosis.Conclusion:5/6 nephrectomy in rats with chronic renal failure BUN(P < 0.01),the Scr(P < 0.01),pathological section residual renal fibrosis,proved that building success.
引文
[1]Piatt R,Roseoe MH,Smith FW,et al.Experimental renal failure.Clin Sci.1952,11:217
[2]慢性肾衰竭大鼠模型检测指标研究进展刘辉;黄国钧;聂娜-《辽宁中医药大学学报》-2010
[3]Andrew S Levey,Josef Coresh.Chronic kidney disease[J].The Lancet.2012(9811)
[4]American Diabetes Association.Executive summary:Standards of medical care in diabetes-2014.Diabetes Care-2014
[5]王清.益肾化浊注射液影响CRF大鼠残余肾中细胞因子含量的实验研究.[J],中华肾脏病杂志2002,27(3):16-17
[6]Ying Shi,Dongtao Wang,Lu Lu,Yi Yin,Ming Wang,Chengjie Li,Jianxin Diao,Yanjing Wang,Lianbo Wei.Ligustilide prevents the apoptosis effects of tumour necrosis factor-alpha during C2C12 cell differentiation[J].International Immunopharmacology.2014(2)
[2]慢性肾衰竭大鼠模型检测指标研究进展刘辉;黄国钧;聂娜-《辽宁中医药大学学报》-2010
[3]Andrew S Levey,Josef Coresh.Chronic kidney disease[J].The Lancet.2012(9811)
[4]American Diabetes Association.Executive summary:Standards of medical care in diabetes-2014.Diabetes Care-2014
[5]王清.益肾化浊注射液影响CRF大鼠残余肾中细胞因子含量的实验研究.[J],中华肾脏病杂志2002,27(3):16-17
[6]Ying Shi,Dongtao Wang,Lu Lu,Yi Yin,Ming Wang,Chengjie Li,Jianxin Diao,Yanjing Wang,Lianbo Wei.Ligustilide prevents the apoptosis effects of tumour necrosis factor-alpha during C2C12 cell differentiation[J].International Immunopharmacology.2014(2)