一株野生梯棱羊肚菌Morchella importuna对重金属的耐受性研究
详细信息 查看官网全文
- 英文论文题名：Tolerance ability of a strain of wild Morchella importuna on heavy metals
- 论文作者：张能 ; 赵苗 ; 谢敬宜 ; 王银 ; 贺新生
- 英文论文作者：ZHANG Neng ; ZHAO Miao ; XIE Jing-Yi ; WANG Yin ; HE Xin-Sheng ; School of Life Science and Engineering ; Southwest University of Science and Technology
- 年：2016
- 作者机构：西南科技大学生命科学与工程学院;
- 论文关键词：梯棱羊肚菌 ; 重金属 ; 浓度梯度 ; 耐受性
- 英文论文关键词：Morchella importuna ; heavy metal ; concentration gradient ; tolerance
- 会议召开时间：2016-08-08
- 会议录名称：2016中国南华野生菌大会资料汇编
- 英文会议录名称：Complation of Information of the Wild Mushroom Conference of Nanhua,China
- 语种：中文
- 分类号：S646.7
- 学会代码：ZGVL
- 会议名称：2016中国南华野生菌大会
- 会议地点：中国云南南华
- 主办单位：中国科学院微生物研究所、中国菌物学会、楚雄州林业局、云南省南华县人民政府
- 学会名称：中国菌物学会
- 页数：11
- 文件大小：1278k
- 原文格式：O
- 会议级别：全国
摘要
羊肚菌是一种珍贵食药用真菌,拥有较高的食药用价值和广阔的市场前景。研究羊肚菌对重金属的耐受性在羊肚菌生理研究、生产实践的指导以及其对重金属吸附研究都具有重要意义。本研究以不同浓度的6种重金属离子单因素作用研究一株野生梯棱羊肚菌Motchella importuna的耐受性。以平板培养法,测量菌落生长长度,计算平均生长速率和生长促进率,绘制生长曲线获取菌丝生长最佳耐受浓度;采用定性方法对气生菌丝浓密、菌核大小和数量进行描述,获得菌核生成最佳耐受浓度。在试验浓度内梯棱羊肚菌对Cr、Mn、Pb、Mo、Cu具有较好的耐受性,这些重金属促进了羊肚菌菌丝生长。对Cd耐受性较差,高浓度Cd抑制菌丝生长。菌丝生长最大促进浓度分别为Cr100.0mg/L;Mn 100.0mg/L;Cd 5.0mg/L;Pb 50.0mg/L;Mo 20.0mg/L;Cu 100.0mg/L;最大生长抑制浓度为Cd 100.0mg/L。促进菌核生成最佳浓度浓度分别为Cr 50.0mg/L、Mn 100.0mg/L、Cd 5.0mg/L、Pb 20.0mg/L、Mo10.0mg/L、Cu 100.0mg/L。
Morelsare one group of precious edible and medicinal fungi,and they have high edible and medicinal value and broad market prospect.To studying on the tolerance ability of morels on heavy metals has great significance in morels physiological research,productive practice and the adsorption ability of morels on heavy metals.The research studies on the tolerance ability of a strain of wild Morchella importuna on six different concentrations of heavy metal ions under the action of single factor.Morchella importuna was cultured by plate culture method.Measuring colony growth length calculated the average growth rate and growth promoting.The optimum concentration of mycelium growth was obtained by drawing growth curve.And the best tolerance concentration of sclerotium generate was described with qualitative methods by mvcelium density,size and number of sclerotium.Morchella importuna had good tolerance ability on Cr,Mn,Pb,Cu and Mo in study concentration range and these heavy metals promoted the mycelium growth.The tolerance ability on Cd was poor,and high concentration of Cd inhibited the growth of mycelium.The maximum promotion concentration were Cr 100.0mg/L;Mn 100.0mg/L;Cd 5.0mg/L;Pb50.0mg/L;Mo 20.0mg/L and Cu 100.0mg/L.The maximum growth inhibition concentration was Cd100.0mg/L.The maximum promotion concentration of sclerotium generates was Cr 50.0mg/L,Mn100.0mg/L,Cd 5.0mg/L,Pb 20.0mg/L,Mo 10.0mg/L and Cu 100.0mg / L.
Morelsare one group of precious edible and medicinal fungi,and they have high edible and medicinal value and broad market prospect.To studying on the tolerance ability of morels on heavy metals has great significance in morels physiological research,productive practice and the adsorption ability of morels on heavy metals.The research studies on the tolerance ability of a strain of wild Morchella importuna on six different concentrations of heavy metal ions under the action of single factor.Morchella importuna was cultured by plate culture method.Measuring colony growth length calculated the average growth rate and growth promoting.The optimum concentration of mycelium growth was obtained by drawing growth curve.And the best tolerance concentration of sclerotium generate was described with qualitative methods by mvcelium density,size and number of sclerotium.Morchella importuna had good tolerance ability on Cr,Mn,Pb,Cu and Mo in study concentration range and these heavy metals promoted the mycelium growth.The tolerance ability on Cd was poor,and high concentration of Cd inhibited the growth of mycelium.The maximum promotion concentration were Cr 100.0mg/L;Mn 100.0mg/L;Cd 5.0mg/L;Pb50.0mg/L;Mo 20.0mg/L and Cu 100.0mg/L.The maximum growth inhibition concentration was Cd100.0mg/L.The maximum promotion concentration of sclerotium generates was Cr 50.0mg/L,Mn100.0mg/L,Cd 5.0mg/L,Pb 20.0mg/L,Mo 10.0mg/L and Cu 100.0mg / L.
引文
An XL,Chou QX,Li T,2008.Growth and Detachment responses of Marcella succulent Hyphen to Single and Comber Pollution of Cd and Pb.Journal of Basic Science and Engine,16(1):35-42(in Chinese)
Chen TS,1995.Manufacture and application of microbial culture media.Beijing:China Agriculture Press,1-642(in Chinese)
Aid YC,Yang ZL,2008.A revised checklist of medicinal fungi in China.Systematic,27:801-824(in Chinese)
Du XH,Chaos Q,Yang ZL,2014.Diversity,evolutionary history and cultivation of morels:a review.Systematic,33(2):183-197(in Chinese)
Hou JR,Li Y,Tolgor,Sun XB,2002.Effect of UV Rays on Hypha Growth of Morchella esculenta,Trichotoma matsumake and Collybia velutipes.Journal of Jilin Agriculture university,24(6):20-24(in Chinese)
Li T,Huang Y,Huang ZJ,2005.Changes of accumulation and distribution of elements and micro-ambient for correctional Hyphen.Ecology and Environment,14(6):870-875
Li WH,Au LL,Chang XH,Chen JD,Dong HX,Ba Tortola,2011.Growth tolerance and accumulation characeeristics of the Mycenae of two macro fungi species to heavy metals.ACT Cosmological Silica,31(5):1240-1248(in Chinese)
Meng YF,2010.Extraction and antioxidant activities of exopolysaccharide and intracellular selenium polysaccharide by Morchella esculenta.Master Thesis,Shandong Agricultural University.6-13(in Chinese)
Michael K,O'Donnell,Folk TJ,2012.Taxonomic revision of true morels(Marcella)in Canada and the United States.Mycology,104(5):1172
Pletsch M,de Araujo BS,Charlwood BV,1999.Novel biotechnological approaches in environmental remediation research.Biotechnology Advances,17:679-687
Rose DJ,May B,1990.Inter specific allotment variation among Marcella app.And its inferences for systematic within the genus.Biochemical systematic and Ecology,18(7-8):475-479
Sanglimsuwan S,Yoshida N,Morinaga T,1993.Resistance to and uptake of heavy metals in mushrooms.Journal of argumentation&Bio engineering,75:112-114
Taskin,Hattie,2013.Detection of Volatile Aroma Compounds of Marcella by Head space Gas Cartographic Mass Electromagnet(HSBC/MS).Tulane Botanic Horowitz Botanical Apocalyptic,41(1):122-125
Die F,Wu PM,Chaos CQ,2014.Biological Characteristics of 7 Strains of Marcella succulent Mycenae.Chinese Agricultural Science Bulletin,30(10):140-147(in Chinese)
Au DY,Chang XH,Duo Y,Li WH,2011.Research Progress on Accumulation of Heavy Metal in Macro-fungi.Edible Fungi of China,30(1):10-13(in Chinese)
Chou QX,An XL,Wei SH,2008.Heavy metal pollution ecology of macro-fungi:research advances and expectation.Chinese Journal of Applied Ecology,19(8):1848-1853(in Chinese)
Thu DX,2008.Research progress of artificial cultivation of Marcella.Edible Fungi of China,27(4):3-5(in Chinese)
安鑫龙,周启星,李婷,2008.羊肚菌菌丝体对镉、铅及其复合污染的生长与富集响应.应用基础与工程科学学报,16(1):35-42.
陈天寿,1995.微生物培养基的制造与应用.北京:中国农业出版社,1-642
戴玉成,杨祝良,2008.中国药用真菌名录及部分名称的修订.菌物学报,27:801-824
杜习慧,赵琪,杨祝良,2014.羊肚菌的多样性、演化历史及栽培研究进展.菌物学报,33(2):183-197
侯集瑞,李玉,图力古尔,孙晓波,2002.紫外线诱导对药用真菌菌丝体生长的影响.吉林农业大学学报,24(6):20-24
李婷,黄艺,黄志基,2005.重金属胁迫下外生菌根真菌Boltholes dueliscs重金属积累分配与生长微环境变化.生态环境,14(6):870-875
李维焕,于兰兰,程显好,陈敬丹,董洪新,图力古尔,2011.两种大型真菌菌丝体对重金属的耐受和富集特性.生态学报,31(5):1240-1248.
孟凡云,2010.羊肚菌胞外多糖与胞内硒多糖抗氧化活性研究.山东农业大学硕士论文.6-13
谢放,吴萍民,赵春巧,2014.7株羊肚菌菌丝的生物学特性研究.中国农学通报,30(10):140-147
于德洋,程显好,罗毅,李维焕,2011.大型真菌重金属富集的研究进展.中国食用菌,30(1):10-13.
周启星,安鑫龙,魏树和,2008.大型真菌重金属污染生态学研究进展与展望.应用生态学报,19(8):1848-1853.
朱斗锡,2008.羊肚菌人工栽培研究进展.中国食用菌,27(4):3-5
Chen TS,1995.Manufacture and application of microbial culture media.Beijing:China Agriculture Press,1-642(in Chinese)
Aid YC,Yang ZL,2008.A revised checklist of medicinal fungi in China.Systematic,27:801-824(in Chinese)
Du XH,Chaos Q,Yang ZL,2014.Diversity,evolutionary history and cultivation of morels:a review.Systematic,33(2):183-197(in Chinese)
Hou JR,Li Y,Tolgor,Sun XB,2002.Effect of UV Rays on Hypha Growth of Morchella esculenta,Trichotoma matsumake and Collybia velutipes.Journal of Jilin Agriculture university,24(6):20-24(in Chinese)
Li T,Huang Y,Huang ZJ,2005.Changes of accumulation and distribution of elements and micro-ambient for correctional Hyphen.Ecology and Environment,14(6):870-875
Li WH,Au LL,Chang XH,Chen JD,Dong HX,Ba Tortola,2011.Growth tolerance and accumulation characeeristics of the Mycenae of two macro fungi species to heavy metals.ACT Cosmological Silica,31(5):1240-1248(in Chinese)
Meng YF,2010.Extraction and antioxidant activities of exopolysaccharide and intracellular selenium polysaccharide by Morchella esculenta.Master Thesis,Shandong Agricultural University.6-13(in Chinese)
Michael K,O'Donnell,Folk TJ,2012.Taxonomic revision of true morels(Marcella)in Canada and the United States.Mycology,104(5):1172
Pletsch M,de Araujo BS,Charlwood BV,1999.Novel biotechnological approaches in environmental remediation research.Biotechnology Advances,17:679-687
Rose DJ,May B,1990.Inter specific allotment variation among Marcella app.And its inferences for systematic within the genus.Biochemical systematic and Ecology,18(7-8):475-479
Sanglimsuwan S,Yoshida N,Morinaga T,1993.Resistance to and uptake of heavy metals in mushrooms.Journal of argumentation&Bio engineering,75:112-114
Taskin,Hattie,2013.Detection of Volatile Aroma Compounds of Marcella by Head space Gas Cartographic Mass Electromagnet(HSBC/MS).Tulane Botanic Horowitz Botanical Apocalyptic,41(1):122-125
Die F,Wu PM,Chaos CQ,2014.Biological Characteristics of 7 Strains of Marcella succulent Mycenae.Chinese Agricultural Science Bulletin,30(10):140-147(in Chinese)
Au DY,Chang XH,Duo Y,Li WH,2011.Research Progress on Accumulation of Heavy Metal in Macro-fungi.Edible Fungi of China,30(1):10-13(in Chinese)
Chou QX,An XL,Wei SH,2008.Heavy metal pollution ecology of macro-fungi:research advances and expectation.Chinese Journal of Applied Ecology,19(8):1848-1853(in Chinese)
Thu DX,2008.Research progress of artificial cultivation of Marcella.Edible Fungi of China,27(4):3-5(in Chinese)
安鑫龙,周启星,李婷,2008.羊肚菌菌丝体对镉、铅及其复合污染的生长与富集响应.应用基础与工程科学学报,16(1):35-42.
陈天寿,1995.微生物培养基的制造与应用.北京:中国农业出版社,1-642
戴玉成,杨祝良,2008.中国药用真菌名录及部分名称的修订.菌物学报,27:801-824
杜习慧,赵琪,杨祝良,2014.羊肚菌的多样性、演化历史及栽培研究进展.菌物学报,33(2):183-197
侯集瑞,李玉,图力古尔,孙晓波,2002.紫外线诱导对药用真菌菌丝体生长的影响.吉林农业大学学报,24(6):20-24
李婷,黄艺,黄志基,2005.重金属胁迫下外生菌根真菌Boltholes dueliscs重金属积累分配与生长微环境变化.生态环境,14(6):870-875
李维焕,于兰兰,程显好,陈敬丹,董洪新,图力古尔,2011.两种大型真菌菌丝体对重金属的耐受和富集特性.生态学报,31(5):1240-1248.
孟凡云,2010.羊肚菌胞外多糖与胞内硒多糖抗氧化活性研究.山东农业大学硕士论文.6-13
谢放,吴萍民,赵春巧,2014.7株羊肚菌菌丝的生物学特性研究.中国农学通报,30(10):140-147
于德洋,程显好,罗毅,李维焕,2011.大型真菌重金属富集的研究进展.中国食用菌,30(1):10-13.
周启星,安鑫龙,魏树和,2008.大型真菌重金属污染生态学研究进展与展望.应用生态学报,19(8):1848-1853.
朱斗锡,2008.羊肚菌人工栽培研究进展.中国食用菌,27(4):3-5