First genetic characterization of Toxoplasma gondii infection in Arctic foxes(Vulpes lagopus) in China
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- 英文论文题名：First genetic characterization of Toxoplasma gondii infection in Arctic foxes(Vulpes lagopus) in China
- 论文作者：Xiaoxuan Zhang ; Wei Cong ; Jiangang Ma ; Zhilong Lou ; Quan Zhao ; Qingfeng Meng ; Aidong Qian ; Xingquan Zhu
- 英文论文作者：Xiaoxuan Zhang ; Wei Cong ; Jiangang Ma ; Zhilong Lou ; Quan Zhao ; Qingfeng Meng ; Aidong Qian ; Xingquan Zhu ; State Key Laboratory of Veterinary Etiological Biology ; Key Laboratory of Veterinary Parasitology of Gansu Province ; Lanzhou Veterinary Research Institute ; Chinese Academy of Agricultural Sciences ; College of Animal Science and Technology ; Jilin Agricultural University ; College of Marine Science ; Shandong University at Weihai ; Jilin Entry-Exit Inspection and Quarantine Bureau
- 年：2016
- 作者机构：State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences;College of Animal Science and Technology, Jilin Agricultural University;College of Marine Science, Shandong University at Weihai;Jilin Entry-Exit Inspection and Quarantine Bureau;
- 英文论文关键词：Toxoplasma gondii ; Arctic fox ; Genetic characterization ; China
- 会议召开时间：2016-08-08
- 会议录名称：中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会论文摘要集
- 英文会议录名称：The Abstracts of the Tenth Symposium for Yong Scientists of China Society of Parasitology
- 语种：英文
- 分类号：S858.92
- 学会代码：JISC
- 会议名称：中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会
- 会议地点：中国四川成都
- 主办单位：中国动物学会寄生虫学专业青年委员会
- 学会名称：中国动物学会寄生虫学专业委员会
- 页数：1
- 文件大小：153k
- 原文格式：D
- 会议级别：全国
摘要
Toxoplasma gondii can infect virtually all warm-blooded animals including foxes. However, little is known of the molecular epidemiology and genotypes of T. gondii infecting foxes in China. Therefore, the present study characterized T. gondii genotypes in foxes in China for the first time. During November 2014 to October 2015, brain tissue samples collected from 264 Arctic foxes(Vulpes lagopus) in Jilin, Heilongjiang and Shandong provinces were used to detect the T. gondii B1 gene by a semi-nested PCR, and the positive samples were genotyped at 10 nuclear loci(i.e., SAG1, alternative SAG2, 5'-and 3'-SAG2, SAG3, L358, BTUB, c22-8, GRA6, c29-2, PK1) and an apicoplast locus(Apico) by multi-locus PCR-RFLP technology. Twenty-one(7.96%) samples from 264 foxes were positive for T. gondii B1 gene. T. gondii infection in male and female foxes was 7.14% and 8.70%, respectively. The highest infection rate(11.86%) was detected in foxes from Shandong, followed by foxes from Jilin(6.49%) and Heilongjiang(2.90%). Two genotypes(Toxo DB#9 and Toxo DB#10) were identified. This is the first genetic characterization of T. gondii from foxes in China, which provides basic data for the surveillance and control of T. gondii infection in foxes, other animals and humans.
Toxoplasma gondii can infect virtually all warm-blooded animals including foxes. However, little is known of the molecular epidemiology and genotypes of T. gondii infecting foxes in China. Therefore, the present study characterized T. gondii genotypes in foxes in China for the first time. During November 2014 to October 2015, brain tissue samples collected from 264 Arctic foxes(Vulpes lagopus) in Jilin, Heilongjiang and Shandong provinces were used to detect the T. gondii B1 gene by a semi-nested PCR, and the positive samples were genotyped at 10 nuclear loci(i.e., SAG1, alternative SAG2, 5'-and 3'-SAG2, SAG3, L358, BTUB, c22-8, GRA6, c29-2, PK1) and an apicoplast locus(Apico) by multi-locus PCR-RFLP technology. Twenty-one(7.96%) samples from 264 foxes were positive for T. gondii B1 gene. T. gondii infection in male and female foxes was 7.14% and 8.70%, respectively. The highest infection rate(11.86%) was detected in foxes from Shandong, followed by foxes from Jilin(6.49%) and Heilongjiang(2.90%). Two genotypes(Toxo DB#9 and Toxo DB#10) were identified. This is the first genetic characterization of T. gondii from foxes in China, which provides basic data for the surveillance and control of T. gondii infection in foxes, other animals and humans.
Toxoplasma gondii can infect virtually all warm-blooded animals including foxes. However, little is known of the molecular epidemiology and genotypes of T. gondii infecting foxes in China. Therefore, the present study characterized T. gondii genotypes in foxes in China for the first time. During November 2014 to October 2015, brain tissue samples collected from 264 Arctic foxes(Vulpes lagopus) in Jilin, Heilongjiang and Shandong provinces were used to detect the T. gondii B1 gene by a semi-nested PCR, and the positive samples were genotyped at 10 nuclear loci(i.e., SAG1, alternative SAG2, 5'-and 3'-SAG2, SAG3, L358, BTUB, c22-8, GRA6, c29-2, PK1) and an apicoplast locus(Apico) by multi-locus PCR-RFLP technology. Twenty-one(7.96%) samples from 264 foxes were positive for T. gondii B1 gene. T. gondii infection in male and female foxes was 7.14% and 8.70%, respectively. The highest infection rate(11.86%) was detected in foxes from Shandong, followed by foxes from Jilin(6.49%) and Heilongjiang(2.90%). Two genotypes(Toxo DB#9 and Toxo DB#10) were identified. This is the first genetic characterization of T. gondii from foxes in China, which provides basic data for the surveillance and control of T. gondii infection in foxes, other animals and humans.
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