单频逆流式超声辅助酶解制备大米ACE抑制肽的影响及其机制研究
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- 英文论文题名：Effects and mechanism of single frequency countercurrent ultrasound(SFCU)assisted enzymatic preparation of angiotensin-I-converting enzyme(ACE)inhibitory peptides from rice protein
- 论文作者：杨雪 ; 马海乐
- 英文论文作者：Yang Xue ; Ma Haile ; School of food and biological engineering ; Jiangsu University
- 年：2016
- 作者机构：江苏大学食品与生物工程学院;
- 论文关键词：超声波 ; 大米蛋白 ; ACE
- 英文论文关键词：ultrasound ; rice protein ; ACE
- 会议召开时间：2016-11-09
- 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
- 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
- 语种：中文
- 分类号：TS201.2
- 学会代码：ZGSP
- 会议名称：中国食品科学技术学会第十三届年会
- 会议地点：中国北京
- 主办单位：中国食品科学技术学会
- 学会名称：中国食品科学技术学会
- 页数：1
- 文件大小：50k
- 原文格式：O
- 会议级别：全国
摘要
本文研究了大米蛋白经过单频逆流式超声波(SFCU)设备预处理,采用碱性蛋白酶酶解后的大米多肽对血管紧张素转换酶(ACE)抑制活性的影响。同时分别采用紫外可见分光光谱仪,傅立叶变换红外光谱仪,荧光分光光度计和扫描电子显微镜对超声后的大米蛋白进行了测定,探讨了超声影响酶解产物活性的机制。结果表明,与未超声相比,超声对大米蛋白的水解度无显著影响(p>0.05),但能显著(p<0.05)提高酶解产物的ACE抑制活性。通过超声后大米蛋白的紫外光谱,红外光谱,表面疏水性的变化表明,超声能提高蛋白二级结构中α-螺旋和降低β-折叠的含量,同时,能显著提高大米蛋白的表面疏水性。扫描电镜的结果显示,超声能使大米蛋白颗粒高度变低,并且颗粒大小更加均匀。因此可以得出,超声通过影响蛋白的结构,使碱性蛋白酶更加容易与酶切位点结合,从而影响其酶解产物的ACE抑制活性。
The effect of single frequency countercurrent ultrasonic(SFCU) on preparation of angiotensin converting enzyme(ACE) inhibitory peptide from rice protein using alcalase was studied.UV-visible spectrophotometer,Fourier transform infrared(FTIR) spectroscopy,fluorescence spectrophotometer and scanning electron microscopy(SEM) were employed to investigate the mechanism of ultrasound infulencing ACE inhibitory activity.The results showed that ultrasound had no significant(p> 0.05) influence on DH of rice protein,but significantly(p < 0.05) improved ACE inhibitory activity of hydrolysate compared to control(without ultrasound).Ultrasound caused unfolding of the α-helical region,followed by the formation of/β-sheet.The results further showed that ultrasound exposed the hydrophobic regions,loosened the protein structure and reduced the protein particles to a more uniform particle size.In conclusion,ultrasound influenced the structure of rice protein,made the alcalase attact restriction sites more frequently so that the ACE inhibitory activity of hydrolysate increased.
The effect of single frequency countercurrent ultrasonic(SFCU) on preparation of angiotensin converting enzyme(ACE) inhibitory peptide from rice protein using alcalase was studied.UV-visible spectrophotometer,Fourier transform infrared(FTIR) spectroscopy,fluorescence spectrophotometer and scanning electron microscopy(SEM) were employed to investigate the mechanism of ultrasound infulencing ACE inhibitory activity.The results showed that ultrasound had no significant(p> 0.05) influence on DH of rice protein,but significantly(p < 0.05) improved ACE inhibitory activity of hydrolysate compared to control(without ultrasound).Ultrasound caused unfolding of the α-helical region,followed by the formation of/β-sheet.The results further showed that ultrasound exposed the hydrophobic regions,loosened the protein structure and reduced the protein particles to a more uniform particle size.In conclusion,ultrasound influenced the structure of rice protein,made the alcalase attact restriction sites more frequently so that the ACE inhibitory activity of hydrolysate increased.
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