泡沫洗手液对致病菌的抑菌作用
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- 英文论文题名：The antimicrobial effect of bubble foam cleanser for hand against pathogen
- 论文作者：朴泰训 ; 徐辅炫 ; 李存桓 ; 安水善
- 英文论文作者：Taehun Park ; BohyunSeo ; JohnHwan Lee ; Susun An ; Amorepacific Corporation R&D Center
- 年：2016
- 作者机构：爱茉莉太平洋研究所;
- 论文关键词：泡沫洗手液 ; 抗菌效果 ; 异丙基甲基苯酚 ; 乙醇
- 英文论文关键词：bubble foam cleanser for hand ; antimicrobial effect ; isopropyl methylphenol ; EtOH
- 会议召开时间：2016-06-15
- 会议录名称：第十一届中国化妆品学术研讨会论文集
- 英文会议录名称：The Proceedings of the 11th Seminar on Cosmetic China
- 语种：中文
- 分类号：TQ658
- 学会代码：ZGXE
- 会议名称：第十一届中国化妆品学术研讨会
- 会议地点：中国上海
- 主办单位：中国香料香精化妆品工业协会
- 学会名称：中国香料香精化妆品工业协会
- 页数：7
- 文件大小：1147k
- 原文格式：O
- 会议级别：全国
摘要
大肠杆菌(E.coli)和金黄色酿脓葡萄球菌(S.aureus)是引起食物中毒最常见的革兰氏阴性菌和阳性菌。用肥皂洗手是防止疾病传播最重要和有效的方法。本项目采用了多种实验方法对各种基质和配方的抗菌性能进行了评估,如最小抑菌浓度(MIC),细菌浓度衰减率测定以及人体临床测试。MIC实验表明,氯化十六烷基铵基吡啶(CPC),2-异丙基-5甲基苯酚(IPMP),对氯间二甲苯酚和十八烷基三甲基氯化铵(STAC)具有很低的抑菌浓度。实验仅对易挥发的乙醇进行了指数衰减测定,与对照组相比,20%乙醇可显著降低E.coli和S.aureus的细菌浓度。此类实验方法可为筛选配方活性成分提供有效的数据支持。最后,本项目对40-46个健康受试者进行了随机的手部临床实验。在使用泡沫洗手液后,含有10%乙醇的产品对E.coli和S.aureus浓度分别达到了3.65和3.94的指数倍衰减,含0.05%CPC和10%乙醇的产品则对两种病原菌分别实现了5.04和3.79的指数倍衰减。
Escherichia coli(E.coli) and Staphylococcus aureus(S.aureus) are the most prevalent species of gram-negative and gram-positive bacteria,respectively,that induce food poisoning.Handwashing with soap is considered the most important and effective way to prevent the spreading the disease.In order to evaluate the antimicrobial effects of substances and formulation,we performed several methods,such as the minimum inhibitory concentration(MIC),declination ratio of bacteria and clinical test.As the result of observed MIC,we found that Cetylpyridinium chloride(CPC),2-isopropyl-5-methylphenol(IPMP),Chloroxylenol and Steartrimonium chloride(STAC) had very low inhibitory concentration,and we carried out Log10 reduction test for only EtOH because EtOH evaporated in incubating.20%EtOH showed statistically significant reduction of E.coliand S.aurues as compared with control.The use of these methods is the most likely to yield useful data on selection for active ingredients of formulation.Finally,we performed randomized clinical test of hands in 54-80 healthy subjects.After using bubble foam cleanser,product(containing 10%EtOH) achieved log reductions of 3.944 in S.aurues,3.653 in E.coli,and product(containing 0.05%CPC and 10%EtOH) was4.530 in S.aurues,5.044 in E.coli.
Escherichia coli(E.coli) and Staphylococcus aureus(S.aureus) are the most prevalent species of gram-negative and gram-positive bacteria,respectively,that induce food poisoning.Handwashing with soap is considered the most important and effective way to prevent the spreading the disease.In order to evaluate the antimicrobial effects of substances and formulation,we performed several methods,such as the minimum inhibitory concentration(MIC),declination ratio of bacteria and clinical test.As the result of observed MIC,we found that Cetylpyridinium chloride(CPC),2-isopropyl-5-methylphenol(IPMP),Chloroxylenol and Steartrimonium chloride(STAC) had very low inhibitory concentration,and we carried out Log10 reduction test for only EtOH because EtOH evaporated in incubating.20%EtOH showed statistically significant reduction of E.coliand S.aurues as compared with control.The use of these methods is the most likely to yield useful data on selection for active ingredients of formulation.Finally,we performed randomized clinical test of hands in 54-80 healthy subjects.After using bubble foam cleanser,product(containing 10%EtOH) achieved log reductions of 3.944 in S.aurues,3.653 in E.coli,and product(containing 0.05%CPC and 10%EtOH) was4.530 in S.aurues,5.044 in E.coli.
引文
[1]UNICEF Malaysia Communications.2002.Global Handwashing Day Planner's Guide,Frequently Asked Questions.
[2]#12
[3]Hadaway,Lynn C.MEd,RNC,CRNI.Skin Flora and Infection.Journal of Infusion Nursing.2003,26(1),44.
[4]C.Bangert,P.M.Brunner,and G.Stingl,Immune functions of the skin.Clinics in Dermatology.2011,29(4),360(2011).
[5]Music Lee,Yunjin Park.Hand washing projects for preventing infectious diseases.Public Health Weekly Report,KCDC 268-277 13(7)
[6]R.N.Michaud,M.B.McGrath,and W.A.Goss.Application of a gloved hand model for multiparameter measurements of skin-degerming activity.Journal of Clinical Microbiology.1976,3(4),406.
[7]M.Roter,W.Kobler,and G.Wewalka.Providoneiodine and chlorhexidinegluconate containing detergents for disinfection of hands.Journal of Hospital Infection.1981,2(3),273.
[8]J.L.Fuls,N.D.Rodgers,G.E.Fischler,J.M.Howard,M.Patel,P.L.Weidner,and M.H.Duran.Alternative hand contamination technique to compare the activities of antimicrobial and nonantimicrobial soaps under different test conditions,Applied Environmental Microbiology.2008,74(12),3739.
[9]M.Rotter,S.sattar,S.Dharan,B.Allegranzi,E.Mathai,and D.Pittet.Method to evaluated the microbicidal activities of hand-rub and hand-wash agents.Journal of Hospital Infection.2009,73(3),191.
[2]#12
[3]Hadaway,Lynn C.MEd,RNC,CRNI.Skin Flora and Infection.Journal of Infusion Nursing.2003,26(1),44.
[4]C.Bangert,P.M.Brunner,and G.Stingl,Immune functions of the skin.Clinics in Dermatology.2011,29(4),360(2011).
[5]Music Lee,Yunjin Park.Hand washing projects for preventing infectious diseases.Public Health Weekly Report,KCDC 268-277 13(7)
[6]R.N.Michaud,M.B.McGrath,and W.A.Goss.Application of a gloved hand model for multiparameter measurements of skin-degerming activity.Journal of Clinical Microbiology.1976,3(4),406.
[7]M.Roter,W.Kobler,and G.Wewalka.Providoneiodine and chlorhexidinegluconate containing detergents for disinfection of hands.Journal of Hospital Infection.1981,2(3),273.
[8]J.L.Fuls,N.D.Rodgers,G.E.Fischler,J.M.Howard,M.Patel,P.L.Weidner,and M.H.Duran.Alternative hand contamination technique to compare the activities of antimicrobial and nonantimicrobial soaps under different test conditions,Applied Environmental Microbiology.2008,74(12),3739.
[9]M.Rotter,S.sattar,S.Dharan,B.Allegranzi,E.Mathai,and D.Pittet.Method to evaluated the microbicidal activities of hand-rub and hand-wash agents.Journal of Hospital Infection.2009,73(3),191.