Angiotensin-I-converting Enzyme Inhibitory Activities and In Vivo Antihypertensive Effects of Sardine Protein Hydrolysate
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- 英文论文题名：Angiotensin-I-converting Enzyme Inhibitory Activities and In Vivo Antihypertensive Effects of Sardine Protein Hydrolysate
- 论文作者：黄嘉成 ; 刘乾月 ; XUE Bin ; CHEN Long ; WANG Yong ; OU Shiyi ; 彭喜春
- 英文论文作者：HUANG Jiacheng ; LIU Qianyue ; XUE Bin ; CHEN Long ; WANG Yong ; OU Shiyi ; PENG Xichun ; Department of Food Science and Engineering ; Jinan University
- 年：2016
- 作者机构：Department of Food Science and Engineering,Jinan University;
- 英文论文关键词：sardine protein hydrolysate ; angiotensin-I-converting enzyme inhibitory activity ; spontaneously hypertensive rats ; normotensive wistar kyoto rats ; antihypertensive effects
- 会议召开时间：2016-12-12
- 会议录名称：“健康食品与功能性食品配料”学术研讨会暨2016年广东省食品学会年会论文集
- 语种：英文
- 分类号：R544.1
- 学会代码：GYSP
- 会议名称：“健康食品与功能性食品配料”学术研讨会暨2016年广东省食品学会年会
- 会议地点：中国广东广州
- 主办单位：广东省食品学会
- 学会名称：广东省食品学会
- 页数：11
- 文件大小：1291k
- 原文格式：O
- 会议级别：全国
摘要
In our previous study,an antihypertensive protein hydrolysate was prepared from sardine.This study aimed to investigate the composition of sardine protein hydrolysate(SPH)and its in vivo antihypertensive effect.SPH was separated sequentially with ultrafiltration and size exclusion chromatography.Fractions with high angiotensin-I-converting enzyme(ACE)inhibitory activity were further analyzed with RP-HPLC and amino acids analysis.Then,SPH was individually oral administrated to spontaneously hypertensive rats(SHR)and normotensive wistar kyoto rats(WKY)for 4 wk.After treatment,the systolic blood pressure(SBP),organ index,oxidative status,serum ACE activity,and serum angiotensin-Ⅱ(ANG-Ⅱ)of all the rats were determined.According to the separation and analysis results,three main fractions with high ACE-inhibitory activity were obtained with different amino acids composition.The animal experiments results showed that SPH could significantly reduce SBP(P<0.05 or P<0.01)within 4 h after a single oral administration.After a chronic oral administration,a steady state of SBP in SHR rats was attained.The heart weight index and left ventricular weight index were significantly reduced(P<0.05)in SPH-treated SHR rats.The malonyldialdehyde(MDA)levels in organs and serum,serum ACE activity,and serum ANG-Ⅱconcentration in SPH-treated SHR rats were significantly lowered(P<0.05 or P<0.01)as compared to their controls.Meanwhile there was no significant effect(P>0.05)on those parameters in WKY rats.These results indicate that SPH can decrease blood pressure in SHR rats and not in WKY rats.
In our previous study,an antihypertensive protein hydrolysate was prepared from sardine.This study aimed to investigate the composition of sardine protein hydrolysate(SPH) and its in vivo antihypertensive effect.SPH was separated sequentially with ultrafiltration and size exclusion chromatography.Fractions with high angiotensin-I-converting enzyme(ACE) inhibitory activity were further analyzed with RP-HPLC and amino acids analysis.Then,SPH was individually oral administrated to spontaneously hypertensive rats(SHR) and normotensive wistar kyoto rats(WKY) for 4 wk.After treatment,the systolic blood pressure(SBP),organ index,oxidative status,serum ACE activity,and serum angiotensin-Ⅱ(ANG-Ⅱ) of all the rats were determined.According to the separation and analysis results,three main fractions with high ACE-inhibitory activity were obtained with different amino acids composition.The animal experiments results showed that SPH could significantly reduce SBP(P < 0.05 or P < 0.01) within 4 h after a single oral administration.After a chronic oral administration,a steady state of SBP in SHR rats was attained.The heart weight index and left ventricular weight index were significantly reduced(P < 0.05) in SPH-treated SHR rats.The malonyldiaidehyde(MDA) levels in organs and serum,serum ACE activity,and serum ANG-II concentration in SPH-treated SHR rats were significantly lowered(P < 0.05 or P < 0.01) as compared to their controls.Meanwhile there was no significant effect(P > 0.05) on those parameters in WKY rats.These results indicate that SPH can decrease blood pressure in SHR rats and not in WKY rats.
In our previous study,an antihypertensive protein hydrolysate was prepared from sardine.This study aimed to investigate the composition of sardine protein hydrolysate(SPH) and its in vivo antihypertensive effect.SPH was separated sequentially with ultrafiltration and size exclusion chromatography.Fractions with high angiotensin-I-converting enzyme(ACE) inhibitory activity were further analyzed with RP-HPLC and amino acids analysis.Then,SPH was individually oral administrated to spontaneously hypertensive rats(SHR) and normotensive wistar kyoto rats(WKY) for 4 wk.After treatment,the systolic blood pressure(SBP),organ index,oxidative status,serum ACE activity,and serum angiotensin-Ⅱ(ANG-Ⅱ) of all the rats were determined.According to the separation and analysis results,three main fractions with high ACE-inhibitory activity were obtained with different amino acids composition.The animal experiments results showed that SPH could significantly reduce SBP(P < 0.05 or P < 0.01) within 4 h after a single oral administration.After a chronic oral administration,a steady state of SBP in SHR rats was attained.The heart weight index and left ventricular weight index were significantly reduced(P < 0.05) in SPH-treated SHR rats.The malonyldiaidehyde(MDA) levels in organs and serum,serum ACE activity,and serum ANG-II concentration in SPH-treated SHR rats were significantly lowered(P < 0.05 or P < 0.01) as compared to their controls.Meanwhile there was no significant effect(P > 0.05) on those parameters in WKY rats.These results indicate that SPH can decrease blood pressure in SHR rats and not in WKY rats.
引文
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[3]Hong F,Ming L,Yi S,et al.The antihypertensive effect of peptides:A novel alternative to drugs?[J].Peptides,2008,29(6):1062-1071.
[4]Garcia-Tejedor A,Castello-Ruiz M,Gimeno-Alcaniz J V,et al.In vivo,antihypertensive mechanism of lactoferrin-derived peptides:Reversion of angiotensinⅠ-and angiotensinⅡ-induced hypertension in Wistar rats[J].Journal of Functional Foods,2015,15:294-300.
[5]Aleman A,Gimenez B,Perezsantin E,et al.Contribution of Leu and Hyp residues to antioxidant and ACE-inhibitory activities of peptide sequences isolated from squid gelatin hydrolysate[J].Food Chemistry,2011,125(2):334-341.
[6]Lin L,Lv S,Li B.Angiotensin-I-converting enzyme(ACE)-inhibitory and antihypertensive properties of squid skin gelatin hydrolysates[J].Food Chemistry,2012,131(1):225-230.
[7]Kim S K,Wijesekara I.Development and biological activities of marine-derived bioactive peptides:A review[J].Journal of Functional Foods,2010,2(1):1-9.
[8]Hernandez-Ledesma B,Del M C M,Recio I.Antihypertensive peptides:production,bioavailability and incorporation into foods.[J].Advances in Colloid&Interface Science,2011,165(1):23-35.
[9]Lafarga T,Hayes M.Bioactive peptides from meat muscle and by-products:generation,functionality and application as functional ingredients.[J].Meat Science,2014,98(2):227-239.
[10]Muguruma M,Ahhmed A M,Katayama K,et al.Identification of pro-drug type ACE inhibitory peptide sourced from porcine myosin B:Evaluation of its antihypertensive effects in vivo[J].Food Chemistry,2009,114(2):516-522.
[11]Jimsheena V K,Gowda L R Arachin derived peptides as selective angiotensin I-converting enzyme(ACE)inhibitors:structure-activity relationship.[J].Peptides,2010,31(6):1165-1176.
[12]Liu X,Zhang M,Jia A,et al.Purification and characterization of angiotensin I converting enzyme inhibitory peptides from jellyfish Rhopilema esculentum[J].Food Research International,2013,50(1):339-343.
[13]Boschin G,Scigliuolo G M,Resta D,et al.ACE-inhibitory activity of enzymatic protein hydrolysates from lupin and other legumes.[J].Food Chemistry,2014,145(7):34-40.
[14]Song H Z,Lin B I,Shun L V,et al.Antihypertensive Effects of Angiotensin-converting Enzyme(ACE)Inhibitory Peptides from Channel Catfish Skin Gelatin[J].Modern Food Science&Technology,2014,30(2).
[15]Wang J,Hu J,Cui J,et al.Purification and identification of a ACE inhibitory peptide from oyster proteins hydrolysate and the antihypertensive effect of hydrolysate in spontaneously hypertensive rats[J].Food Chemistry,2008,111(2):302-308.
[16]Romero J C,Reckelhoff J F.State-of-the-Art lecture.Role of angiotensin and oxidative stress in essential hypertension.[J].Hypertension,1999,34(4 Pt 2):943-9.
[17]Pedro B J,Covas M S,Rubies P J.Decreased endogenous antioxidant enzymatic status in essential hypertension.[J].Journal of Human Hypertension,2000,14(6):343-5.
[18]Zimmerman M C,Lazartigues E,Sharma R V,et al.Hypertension caused by angiotensinⅡinfusion involves increased superoxide production in the central nervous system.[J].Circulation Research,2004,95(2):210-6.
[19]Valko M,Leibfritz D,Moncol J,et al.Free radicals and antioxidants in normal physiological functions and human disease.[J].International Journal of Biochemistry&Cell Biology,2007,39(1):44-84.
[20]Tse W Y,Maxwell S R,Thomason H,et al.Antioxidant status in controlled and uncontrolled hypertension and its relationship to endothelial damage.[J].Journal of Human Hypertension,1994,8(11):843-9.
[21]Lacy F,O'Connor D T,Schmid-Schonbein G W.Plasma hydrogen peroxide production in hypertensives and normotensive subjects at genetic risk of hypertension.[J].Journal of Hypertension,1998,16(3):291-303.
[22]Di B R,Mullen A M,Bolton D,et al.Assessment of the angiotensin-I-converting enzyme(ACE-I)inhibitory and antioxidant activities of hydrolysates of bovine brisket sarcoplasmic proteins produced by papain and characterisation of associated bioactive peptidic fractions.[J].Meat Science,2012,90(1):226-235.
[23]Orona-Tamayo D,Valverde M E,Nieto-Rendon B,et al.Inhibitory activity of chia(Salvia hispanica,L.)protein fractions against angiotensin I-converting enzyme and antioxidant capacity[J].Lebensmittel-Wissenschaft und-Technologie,2015,64(1):236-242.
[24]Seiquer I,Aspe T,Perezgranados A M,et al.Consumption of raw and fried sardine(Clupea pilchardus)as protein source of diets:effects on iron metabolism in rats[J].Journal of the Science of Food andAgriculture,2002,82(13):1497-1503.
[25]Jia-cheng H,Xi-chun P.2015.Two-step enzymatic hydrolysis for preparation of ACE-inhibitory peptides from sardine.Science and Technology of Food Industry 36(18):295-300.
[26]Arihara K,Nakashima Y,Mukai T,et al.Peptide inhibitors for angiotensin I-converting enzyme from enzymatic hydrolysates of porcine skeletal muscle proteins.[J].Meat Science,2001,57(3):319-324.
[27]Wu H,He H L,Chen X L,et al.Purification and identification of novel angiotensin-I-converting enzyme inhibitory peptides from shark meat hydrolysate[J].Process Biochemistry,2008,43(4):457-461.
[28]Zhang Y.Production and characteristics of protein hydrolysates from capelin(Mallotus villosus)[J].Food Chemistry,1995,53(95):285-293.
[29]Frohlich E D,Apstein C,Chobanian A V,et al.The heart in hypertension.[J].New England Journal of Medicine,1991,327(14):1396-1400.
[30]Intengan H D,Thibault G,Li J S,et al.Resistance artery mechanics,structure,and extracellular components in spontaneously hypertensive rats:effects of angiotensin receptor antagonism and converting enzyme inhibition.[J].Circulation,1999,100(22):2267-75.
[31]Suzuki H,Swei A,Zweifach B W,et al.In vivo evidence for microvascular oxidative stress in spontaneously hypertensive rats.Hydroethidine microfluorography.[J].Hypertension,1995,25(25):1083-9.
[32]Kitts D D,Yuan Y V,Godin D V.Plasma and lipoprotein lipid composition and hepatic antioxidant status in spontaneously hypertensive(SHR)and normotensive(WKY)rats.[J].Canadian Journal of Physiology&Pharmacology,1998,76(2):202-9.
[33]Duarte J,Perezpalencia R,Vargas F,et al.Antihypertensive effects of the flavonoid quercetin in spontaneously hypertensive rats.[J].British Journal of Pharmacology,2001,61(1):67-75.
[34]Touyz R M,Briones A M.Reactive oxygen species and vascular biology:implications in human hypertension[J].Hypertension Research Official Journal of the Japanese Society of Hypertension,2011,34(1):5-14.
[35]Montezano A C,Touyz R M.Molecular mechanisms of hypertension-reactive oxygen species and antioxidants:a basic science update for the clinician.[J].Canadian Journal of Cardiology,2012,28(3):288-95.
[2]Osamor P E,Owumi B E.Factors associated with treatment compliance in hypertension in southwest Nigeria.[J].Journal of Health Population&Nutrition,2011,29(6):619-628.
[3]Hong F,Ming L,Yi S,et al.The antihypertensive effect of peptides:A novel alternative to drugs?[J].Peptides,2008,29(6):1062-1071.
[4]Garcia-Tejedor A,Castello-Ruiz M,Gimeno-Alcaniz J V,et al.In vivo,antihypertensive mechanism of lactoferrin-derived peptides:Reversion of angiotensinⅠ-and angiotensinⅡ-induced hypertension in Wistar rats[J].Journal of Functional Foods,2015,15:294-300.
[5]Aleman A,Gimenez B,Perezsantin E,et al.Contribution of Leu and Hyp residues to antioxidant and ACE-inhibitory activities of peptide sequences isolated from squid gelatin hydrolysate[J].Food Chemistry,2011,125(2):334-341.
[6]Lin L,Lv S,Li B.Angiotensin-I-converting enzyme(ACE)-inhibitory and antihypertensive properties of squid skin gelatin hydrolysates[J].Food Chemistry,2012,131(1):225-230.
[7]Kim S K,Wijesekara I.Development and biological activities of marine-derived bioactive peptides:A review[J].Journal of Functional Foods,2010,2(1):1-9.
[8]Hernandez-Ledesma B,Del M C M,Recio I.Antihypertensive peptides:production,bioavailability and incorporation into foods.[J].Advances in Colloid&Interface Science,2011,165(1):23-35.
[9]Lafarga T,Hayes M.Bioactive peptides from meat muscle and by-products:generation,functionality and application as functional ingredients.[J].Meat Science,2014,98(2):227-239.
[10]Muguruma M,Ahhmed A M,Katayama K,et al.Identification of pro-drug type ACE inhibitory peptide sourced from porcine myosin B:Evaluation of its antihypertensive effects in vivo[J].Food Chemistry,2009,114(2):516-522.
[11]Jimsheena V K,Gowda L R Arachin derived peptides as selective angiotensin I-converting enzyme(ACE)inhibitors:structure-activity relationship.[J].Peptides,2010,31(6):1165-1176.
[12]Liu X,Zhang M,Jia A,et al.Purification and characterization of angiotensin I converting enzyme inhibitory peptides from jellyfish Rhopilema esculentum[J].Food Research International,2013,50(1):339-343.
[13]Boschin G,Scigliuolo G M,Resta D,et al.ACE-inhibitory activity of enzymatic protein hydrolysates from lupin and other legumes.[J].Food Chemistry,2014,145(7):34-40.
[14]Song H Z,Lin B I,Shun L V,et al.Antihypertensive Effects of Angiotensin-converting Enzyme(ACE)Inhibitory Peptides from Channel Catfish Skin Gelatin[J].Modern Food Science&Technology,2014,30(2).
[15]Wang J,Hu J,Cui J,et al.Purification and identification of a ACE inhibitory peptide from oyster proteins hydrolysate and the antihypertensive effect of hydrolysate in spontaneously hypertensive rats[J].Food Chemistry,2008,111(2):302-308.
[16]Romero J C,Reckelhoff J F.State-of-the-Art lecture.Role of angiotensin and oxidative stress in essential hypertension.[J].Hypertension,1999,34(4 Pt 2):943-9.
[17]Pedro B J,Covas M S,Rubies P J.Decreased endogenous antioxidant enzymatic status in essential hypertension.[J].Journal of Human Hypertension,2000,14(6):343-5.
[18]Zimmerman M C,Lazartigues E,Sharma R V,et al.Hypertension caused by angiotensinⅡinfusion involves increased superoxide production in the central nervous system.[J].Circulation Research,2004,95(2):210-6.
[19]Valko M,Leibfritz D,Moncol J,et al.Free radicals and antioxidants in normal physiological functions and human disease.[J].International Journal of Biochemistry&Cell Biology,2007,39(1):44-84.
[20]Tse W Y,Maxwell S R,Thomason H,et al.Antioxidant status in controlled and uncontrolled hypertension and its relationship to endothelial damage.[J].Journal of Human Hypertension,1994,8(11):843-9.
[21]Lacy F,O'Connor D T,Schmid-Schonbein G W.Plasma hydrogen peroxide production in hypertensives and normotensive subjects at genetic risk of hypertension.[J].Journal of Hypertension,1998,16(3):291-303.
[22]Di B R,Mullen A M,Bolton D,et al.Assessment of the angiotensin-I-converting enzyme(ACE-I)inhibitory and antioxidant activities of hydrolysates of bovine brisket sarcoplasmic proteins produced by papain and characterisation of associated bioactive peptidic fractions.[J].Meat Science,2012,90(1):226-235.
[23]Orona-Tamayo D,Valverde M E,Nieto-Rendon B,et al.Inhibitory activity of chia(Salvia hispanica,L.)protein fractions against angiotensin I-converting enzyme and antioxidant capacity[J].Lebensmittel-Wissenschaft und-Technologie,2015,64(1):236-242.
[24]Seiquer I,Aspe T,Perezgranados A M,et al.Consumption of raw and fried sardine(Clupea pilchardus)as protein source of diets:effects on iron metabolism in rats[J].Journal of the Science of Food andAgriculture,2002,82(13):1497-1503.
[25]Jia-cheng H,Xi-chun P.2015.Two-step enzymatic hydrolysis for preparation of ACE-inhibitory peptides from sardine.Science and Technology of Food Industry 36(18):295-300.
[26]Arihara K,Nakashima Y,Mukai T,et al.Peptide inhibitors for angiotensin I-converting enzyme from enzymatic hydrolysates of porcine skeletal muscle proteins.[J].Meat Science,2001,57(3):319-324.
[27]Wu H,He H L,Chen X L,et al.Purification and identification of novel angiotensin-I-converting enzyme inhibitory peptides from shark meat hydrolysate[J].Process Biochemistry,2008,43(4):457-461.
[28]Zhang Y.Production and characteristics of protein hydrolysates from capelin(Mallotus villosus)[J].Food Chemistry,1995,53(95):285-293.
[29]Frohlich E D,Apstein C,Chobanian A V,et al.The heart in hypertension.[J].New England Journal of Medicine,1991,327(14):1396-1400.
[30]Intengan H D,Thibault G,Li J S,et al.Resistance artery mechanics,structure,and extracellular components in spontaneously hypertensive rats:effects of angiotensin receptor antagonism and converting enzyme inhibition.[J].Circulation,1999,100(22):2267-75.
[31]Suzuki H,Swei A,Zweifach B W,et al.In vivo evidence for microvascular oxidative stress in spontaneously hypertensive rats.Hydroethidine microfluorography.[J].Hypertension,1995,25(25):1083-9.
[32]Kitts D D,Yuan Y V,Godin D V.Plasma and lipoprotein lipid composition and hepatic antioxidant status in spontaneously hypertensive(SHR)and normotensive(WKY)rats.[J].Canadian Journal of Physiology&Pharmacology,1998,76(2):202-9.
[33]Duarte J,Perezpalencia R,Vargas F,et al.Antihypertensive effects of the flavonoid quercetin in spontaneously hypertensive rats.[J].British Journal of Pharmacology,2001,61(1):67-75.
[34]Touyz R M,Briones A M.Reactive oxygen species and vascular biology:implications in human hypertension[J].Hypertension Research Official Journal of the Japanese Society of Hypertension,2011,34(1):5-14.
[35]Montezano A C,Touyz R M.Molecular mechanisms of hypertension-reactive oxygen species and antioxidants:a basic science update for the clinician.[J].Canadian Journal of Cardiology,2012,28(3):288-95.