Membrane-associated GRP78 helps subgroup J Avian leucosis virus enter cells
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- 英文论文题名：Membrane-associated GRP78 helps subgroup J Avian leucosis virus enter cells
- 论文作者：Lin Wang ; Mei Mei ; Aijian Qin ; Yuelong Liu ; Jianqiang Ye ; Kun Qian ; Hongxia Shao
- 英文论文作者：Lin Wang ; Mei Mei ; Aijian Qin ; Yuelong Liu ; Jianqiang Ye ; Kun Qian ; Hongxia Shao ; Ministry of Education Key Lab for Avian Preventive Medicine ; Yangzhou University ; Key Laboratory of Jiangsu Preventive Veterinary Medicine ; Yangzhou University ; Yangzhou University ; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses ; Jiangsu Key Lab of Zoonosis
- 年：2016
- 作者机构：Ministry of Education Key Lab for Avian Preventive Medicine,Yangzhou University;Key Laboratory of Jiangsu Preventive Veterinary Medicine,Yangzhou University,Yangzhou University;Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses;Jiangsu Key Lab of Zoonosis;
- 英文论文关键词：chGRP78 ; avian leucosis virus subgroup J ; virus entry
- 会议召开时间：2016-09-18
- 会议录名称：第六届中国兽药大会论文集
- 英文会议录名称：Proceedings of 2016 the 6th China Congress of Veterinary Medicine
- 语种：英文
- 分类号：S852.65
- 学会代码：ZGXJ
- 会议名称：第六届中国兽药大会
- 会议地点：中国四川成都
- 主办单位：中国兽医药品监察所、中国兽药协会
- 学会名称：中国畜牧兽医学会
- 页数：1
- 文件大小：59k
- 原文格式：O
- 会议级别：全国
摘要
We previously identified chicken Annexin A2(chANXA2) as a novel receptor for retrovirus avian leucosis virus subgroup J(ALV-J),using a DF1 cell line expressing the viral envelope(env) protein.To further probe whether other proteins participate in virus infection,we investigated several host proteins from co-immunoprecipitation with the DF1 cell line expressing viral env.Mass spectrometry analysis indicated that the chicken glucose-regulation protein 78(chGRP78) of the DF1 membrane interacted with the ALV-J env protein.The results revealed that antibodies or siRNA to chGRP78 significantly inhibited ALV-J infection and replication,and over-expression of chGRP78 enabled the entry of ALV-J into non-susceptible cells.Taken together,these results are the first to report that chGRP78 functions to help ALV-J enter cells.
We previously identified chicken Annexin A2(chANXA2) as a novel receptor for retrovirus avian leucosis virus subgroup J(ALV-J),using a DF1 cell line expressing the viral envelope(env) protein.To further probe whether other proteins participate in virus infection,we investigated several host proteins from co-immunoprecipitation with the DF1 cell line expressing viral env.Mass spectrometry analysis indicated that the chicken glucose-regulation protein 78(chGRP78) of the DF1 membrane interacted with the ALV-J env protein.The results revealed that antibodies or siRNA to chGRP78 significantly inhibited ALV-J infection and replication,and over-expression of chGRP78 enabled the entry of ALV-J into non-susceptible cells.Taken together,these results are the first to report that chGRP78 functions to help ALV-J enter cells.
We previously identified chicken Annexin A2(chANXA2) as a novel receptor for retrovirus avian leucosis virus subgroup J(ALV-J),using a DF1 cell line expressing the viral envelope(env) protein.To further probe whether other proteins participate in virus infection,we investigated several host proteins from co-immunoprecipitation with the DF1 cell line expressing viral env.Mass spectrometry analysis indicated that the chicken glucose-regulation protein 78(chGRP78) of the DF1 membrane interacted with the ALV-J env protein.The results revealed that antibodies or siRNA to chGRP78 significantly inhibited ALV-J infection and replication,and over-expression of chGRP78 enabled the entry of ALV-J into non-susceptible cells.Taken together,these results are the first to report that chGRP78 functions to help ALV-J enter cells.
引文