Anti-tumor activity of xerocomic acid from Chroogomphus rutilus in human liver cancer cells in vivo and in vitro
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- 英文论文题名：Anti-tumor activity of xerocomic acid from Chroogomphus rutilus in human liver cancer cells in vivo and in vitro
- 论文作者：Qinqin Gao ; Haiying Bao ; Zhenzhuo Li ; Tolgor Bao ; Yu Li
- 英文论文作者：Qinqin Gao ; Haiying Bao ; Zhenzhuo Li ; Tolgor Bao ; Yu Li ; College of Traditional Chinese Medicinal Material ; Jilin Agricultural University ; Engineering Research Centre of Edible and Medicinal Fungi ; Ministry of Education ; Jilin Agricultural University
- 年：2016
- 作者机构：College of Traditional Chinese Medicinal Material,Jilin Agricultural University;Engineering Research Centre of Edible and Medicinal Fungi,Ministry of Education,Jilin Agricultural University;
- 英文论文关键词：Chroogomphus rutilus ; xerocomic acid ; antitumor ; Hep G2 cell ; liver cancer ; apoptosis
- 会议召开时间：2016-08-19
- 会议录名称：中国菌物学会2016年学术年会论文摘要集
- 英文会议录名称：Abstracts of 2016 Mycological Society of China Annual Meeting
- 语种：英文
- 分类号：R285
- 学会代码：ZGVL
- 会议名称：中国菌物学会2016年学术年会
- 会议地点：中国福建福州
- 主办单位：中国菌物学会
- 学会名称：中国菌物学会
- 页数：1
- 文件大小：809k
- 原文格式：D
- 会议级别：全国
摘要
Context:Liver cancer is common in developing countries and it is the most common cancer type in some countries.Existing chemotherapeutic agents are mainly traditional cytotoxic drugs,which will produce toxic side effects,without desired therapeutic effect.Therefore,it is necessary to develop anti-tumor drugs with high efficiency and low toxicity,especially natural active ingredients isolated from natural materials have become the current research focus.Objective:The purpose of this experiment was to screen natural monomer compounds with antitumor activity from Chroogomphus rutilus through H22 tumor-burdened mice in vivo,and combined with antitumor effect in vitro to elucidate its role in the treatment of liver cancer process.The anti-tumor mechanism and possible pathways of apoptosis were further study.Materials and methods:Antitumor activity of xerocomic acid from the fruiting body of Chroogomphus rutilus was evaluated by Sulforhodamine B(SRB) proliferation assay,cell morphological observation,and annexin V-FITC/PI double staining tests.Results:Our present study has shown that the tumor inhibition rate of xerocomic acid highly amounted to(57.71%) closed to the positive group(57.89%) and xerocomic acid has a broad spectrum of tumor suppression effect in a dose-dependent manner.With detection of Bcl-2,Bax,Cyt-c and PARP protein expression level in the Hep G2 cell,preliminary judgment was that xerocomic acid induced Hep G2 liver cancer cell apoptosis through mitochondrial endogenous approaches.Discussion and conclusion:This study has a good application prospect and practical significance for the research and development of antitumor drugs with high efficiency and low toxicity.
Context:Liver cancer is common in developing countries and it is the most common cancer type in some countries.Existing chemotherapeutic agents are mainly traditional cytotoxic drugs,which will produce toxic side effects,without desired therapeutic effect.Therefore,it is necessary to develop anti-tumor drugs with high efficiency and low toxicity,especially natural active ingredients isolated from natural materials have become the current research focus.Objective:The purpose of this experiment was to screen natural monomer compounds with antitumor activity from Chroogomphus rutilus through H22 tumor-burdened mice in vivo,and combined with antitumor effect in vitro to elucidate its role in the treatment of liver cancer process.The anti-tumor mechanism and possible pathways of apoptosis were further study.Materials and methods:Antitumor activity of xerocomic acid from the fruiting body of Chroogomphus rutilus was evaluated by Sulforhodamine B(SRB) proliferation assay,cell morphological observation,and annexin V-FITC/PI double staining tests.Results:Our present study has shown that the tumor inhibition rate of xerocomic acid highly amounted to(57.71%) closed to the positive group(57.89%) and xerocomic acid has a broad spectrum of tumor suppression effect in a dose-dependent manner.With detection of Bcl-2,Bax,Cyt-c and PARP protein expression level in the Hep G2 cell,preliminary judgment was that xerocomic acid induced Hep G2 liver cancer cell apoptosis through mitochondrial endogenous approaches.Discussion and conclusion:This study has a good application prospect and practical significance for the research and development of antitumor drugs with high efficiency and low toxicity.
Context:Liver cancer is common in developing countries and it is the most common cancer type in some countries.Existing chemotherapeutic agents are mainly traditional cytotoxic drugs,which will produce toxic side effects,without desired therapeutic effect.Therefore,it is necessary to develop anti-tumor drugs with high efficiency and low toxicity,especially natural active ingredients isolated from natural materials have become the current research focus.Objective:The purpose of this experiment was to screen natural monomer compounds with antitumor activity from Chroogomphus rutilus through H22 tumor-burdened mice in vivo,and combined with antitumor effect in vitro to elucidate its role in the treatment of liver cancer process.The anti-tumor mechanism and possible pathways of apoptosis were further study.Materials and methods:Antitumor activity of xerocomic acid from the fruiting body of Chroogomphus rutilus was evaluated by Sulforhodamine B(SRB) proliferation assay,cell morphological observation,and annexin V-FITC/PI double staining tests.Results:Our present study has shown that the tumor inhibition rate of xerocomic acid highly amounted to(57.71%) closed to the positive group(57.89%) and xerocomic acid has a broad spectrum of tumor suppression effect in a dose-dependent manner.With detection of Bcl-2,Bax,Cyt-c and PARP protein expression level in the Hep G2 cell,preliminary judgment was that xerocomic acid induced Hep G2 liver cancer cell apoptosis through mitochondrial endogenous approaches.Discussion and conclusion:This study has a good application prospect and practical significance for the research and development of antitumor drugs with high efficiency and low toxicity.
引文