Label-free proteomic analysis of placental proteins during Toxoplasma gondii infection
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- 英文论文题名：Label-free proteomic analysis of placental proteins during Toxoplasma gondii infection
- 论文作者：Hu Xuemei ; Jiao Fang ; Zhang Dan ; Jiang Mengqi ; Mi Jia ; Liu Xianbing ; Zhang Haixia ; Hu Zhengping ; Xu Xiaoyan
- 英文论文作者：Hu Xuemei ; Jiao Fang ; Zhang Dan ; Jiang Mengqi ; Mi Jia ; Liu Xianbing ; Zhang Haixia ; Hu Zhengping ; Xu Xiaoyan ; Department of Immunology ; Binzhou Medical University ; Medicine & Pharmacy Research Center ; Binzhou Medical University ; epartment of Gynecology and Obstetrics ; Binzhou Affiliated Hospital of Binzhou Medical University
- 年：2016
- 作者机构：Department of Immunology,Binzhou Medical University;Medicine & Pharmacy Research Center,Binzhou Medical University;epartment of Gynecology and Obstetrics,Binzhou Affiliated Hospital of Binzhou Medical University;
- 英文论文关键词：Annexins ; Placenta ; Protease inhibitor ; Proteomic ; Toxoplasma gondii
- 会议召开时间：2016-11-04
- 会议录名称：第十一届全国免疫学学术大会摘要汇编
- 英文会议录名称：Abstracts of 2016 CSI Congress on Immunology
- 语种：英文
- 分类号：R714.251
- 学会代码：IGMD
- 会议名称：第十一届全国免疫学学术大会
- 会议地点：中国安徽合肥
- 主办单位：中国免疫学会
- 学会名称：中国免疫学会
- 页数：1
- 文件大小：1329k
- 原文格式：D
- 会议级别：全国
摘要
Aims: Toxoplasma gondii is a ubiquitous, obligate intracellular parasite capable of crossing the placental barrier and causing spontaneous abortion, preterm labor, or significant disease in the surviving neonate. We aim to better understand molecular mechanisms underlying abnormal pregnancy outcomes caused by T. gondii. Methods: Placental proteins extracted from T. gondii-infected and-uninfected mice were comparatively analyzed using label-free liquid chromatography-tandem mass spectrometry. Quantitative real-time polymerase chain reaction, western blotting, and immunohistochemical staining were used to validate the results of the proteomic analysis. Results: Significant difference was observed in the expression of 58 out of 792 proteins in infected placentas(p<0.05) compared with that in uninfected placentas. Some placental proteins differentially expressed in infected and uninfected mice were found to be associated with several different biological processes of pregnancy, particularly with trophoblast invasion and placental development. Conclusion: The results provide possible novel insights into the molecular mechanisms for abnormal pregnancy outcomes associated with T. gondii infection.
Aims: Toxoplasma gondii is a ubiquitous, obligate intracellular parasite capable of crossing the placental barrier and causing spontaneous abortion, preterm labor, or significant disease in the surviving neonate. We aim to better understand molecular mechanisms underlying abnormal pregnancy outcomes caused by T. gondii. Methods: Placental proteins extracted from T. gondii-infected and-uninfected mice were comparatively analyzed using label-free liquid chromatography-tandem mass spectrometry. Quantitative real-time polymerase chain reaction, western blotting, and immunohistochemical staining were used to validate the results of the proteomic analysis. Results: Significant difference was observed in the expression of 58 out of 792 proteins in infected placentas(p<0.05) compared with that in uninfected placentas. Some placental proteins differentially expressed in infected and uninfected mice were found to be associated with several different biological processes of pregnancy, particularly with trophoblast invasion and placental development. Conclusion: The results provide possible novel insights into the molecular mechanisms for abnormal pregnancy outcomes associated with T. gondii infection.
Aims: Toxoplasma gondii is a ubiquitous, obligate intracellular parasite capable of crossing the placental barrier and causing spontaneous abortion, preterm labor, or significant disease in the surviving neonate. We aim to better understand molecular mechanisms underlying abnormal pregnancy outcomes caused by T. gondii. Methods: Placental proteins extracted from T. gondii-infected and-uninfected mice were comparatively analyzed using label-free liquid chromatography-tandem mass spectrometry. Quantitative real-time polymerase chain reaction, western blotting, and immunohistochemical staining were used to validate the results of the proteomic analysis. Results: Significant difference was observed in the expression of 58 out of 792 proteins in infected placentas(p<0.05) compared with that in uninfected placentas. Some placental proteins differentially expressed in infected and uninfected mice were found to be associated with several different biological processes of pregnancy, particularly with trophoblast invasion and placental development. Conclusion: The results provide possible novel insights into the molecular mechanisms for abnormal pregnancy outcomes associated with T. gondii infection.
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