The role of SND1 ubiquitination in the chemosensitivity of hepatocellular carcinoma
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- 英文论文题名：The role of SND1 ubiquitination in the chemosensitivity of hepatocellular carcinoma
- 论文作者：Cui Xiaoteng ; Qian Baoxin ; Zhao Chunyan ; Fu Xue ; Gao Xingjie ; Yang Jie
- 英文论文作者：Cui Xiaoteng ; Qian Baoxin ; Zhao Chunyan ; Fu Xue ; Gao Xingjie ; Yang Jie ; Tianjin medical university
- 年：2016
- 作者机构：Tianjin medical university;
- 英文论文关键词：SND1 ; hepatocellular carcinoma ; ubiquitination ; chemosensitivity
- 会议召开时间：2016-11-04
- 会议录名称：第十一届全国免疫学学术大会摘要汇编
- 英文会议录名称：Abstracts of 2016 CSI Congress on Immunology
- 语种：英文
- 分类号：R735.7
- 学会代码：IGMD
- 会议名称：第十一届全国免疫学学术大会
- 会议地点：中国安徽合肥
- 主办单位：中国免疫学会
- 学会名称：中国免疫学会
- 页数：2
- 文件大小：1294k
- 原文格式：D
- 会议级别：全国
摘要
Purpose: The reduced chemosensitivity of Hepatocellular carcinomas(HCC) often contributes to the poor clinical efficacy of chemotherapy for patients with HCC. Staphylococcal nuclease and tudor domain containing 1(SND1), a multifunctional protein, is involved in cell cycle, DNA damage repair and other cellular physiological processes. Here, we investigated the potential role of SND1 in chemosensitivity of HCC.Methods: Several assays, such as Annexin V-FITC/PI, CRISPR/Cas9 n double-nick system, Mass spectrometry or western blotting, were performed.Results: We found that SND1 protein was associated with the sensitivity of SMMC-7721 cells to chemotherapeutic agents 5-Fu. SMMC-7721 cells were more sensitive to 5-Fu after the knock-out of SND1 gene by CRISPR/Cas9 n double-nick system. In addition, 48 h after treated with 25 μg/m L Cycloheximide(an inhibitor of protein biosynthesis), SND1 protein was degraded. This process could be inhibited by MG-132(a proteasome inhibitor), and the ubiquitin signal of SND1 protein could be detected as well. Mass spectrometry data further showed that SND1 protein was associated with a series of chemosensitivity-associated proteins, such as Ubiquitin-Like With PHD And Ring Finger Domains 1(UHRF1), Poly(ADP-Ribose) Polymerase 1(PABP1) and X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 6(XRCC6).Conclusion: These results suggested that SND1 may affect the sensitivity of HCC cells to chemotherapeutic agents by means of ubiquitination modification.
Purpose: The reduced chemosensitivity of Hepatocellular carcinomas(HCC) often contributes to the poor clinical efficacy of chemotherapy for patients with HCC. Staphylococcal nuclease and tudor domain containing 1(SND1), a multifunctional protein, is involved in cell cycle, DNA damage repair and other cellular physiological processes. Here, we investigated the potential role of SND1 in chemosensitivity of HCC.Methods: Several assays, such as Annexin V-FITC/PI, CRISPR/Cas9 n double-nick system, Mass spectrometry or western blotting, were performed.Results: We found that SND1 protein was associated with the sensitivity of SMMC-7721 cells to chemotherapeutic agents 5-Fu. SMMC-7721 cells were more sensitive to 5-Fu after the knock-out of SND1 gene by CRISPR/Cas9 n double-nick system. In addition, 48 h after treated with 25 μg/m L Cycloheximide(an inhibitor of protein biosynthesis), SND1 protein was degraded. This process could be inhibited by MG-132(a proteasome inhibitor), and the ubiquitin signal of SND1 protein could be detected as well. Mass spectrometry data further showed that SND1 protein was associated with a series of chemosensitivity-associated proteins, such as Ubiquitin-Like With PHD And Ring Finger Domains 1(UHRF1), Poly(ADP-Ribose) Polymerase 1(PABP1) and X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 6(XRCC6).Conclusion: These results suggested that SND1 may affect the sensitivity of HCC cells to chemotherapeutic agents by means of ubiquitination modification.
Purpose: The reduced chemosensitivity of Hepatocellular carcinomas(HCC) often contributes to the poor clinical efficacy of chemotherapy for patients with HCC. Staphylococcal nuclease and tudor domain containing 1(SND1), a multifunctional protein, is involved in cell cycle, DNA damage repair and other cellular physiological processes. Here, we investigated the potential role of SND1 in chemosensitivity of HCC.Methods: Several assays, such as Annexin V-FITC/PI, CRISPR/Cas9 n double-nick system, Mass spectrometry or western blotting, were performed.Results: We found that SND1 protein was associated with the sensitivity of SMMC-7721 cells to chemotherapeutic agents 5-Fu. SMMC-7721 cells were more sensitive to 5-Fu after the knock-out of SND1 gene by CRISPR/Cas9 n double-nick system. In addition, 48 h after treated with 25 μg/m L Cycloheximide(an inhibitor of protein biosynthesis), SND1 protein was degraded. This process could be inhibited by MG-132(a proteasome inhibitor), and the ubiquitin signal of SND1 protein could be detected as well. Mass spectrometry data further showed that SND1 protein was associated with a series of chemosensitivity-associated proteins, such as Ubiquitin-Like With PHD And Ring Finger Domains 1(UHRF1), Poly(ADP-Ribose) Polymerase 1(PABP1) and X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 6(XRCC6).Conclusion: These results suggested that SND1 may affect the sensitivity of HCC cells to chemotherapeutic agents by means of ubiquitination modification.
引文