喷雾干燥细菌素的生物学活性研究
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- 英文论文题名：Study on the Biological Activity of a Spray Dried Bacteriocin
- 论文作者：卫旭彪 ; 武如娟 ; 郑召君 ; 马广 ; 廖秀冬 ; 张日俊
- 英文论文作者：WEI Xu-biao ; WU Ru-juan ; ZHENG Zhao-jun ; MA Guang ; LIAO Xiu-dong ; ZHANG Ri-jun ; Laboratory of Feed Biotechnology ; State Key Laboratory of Animal Nutrition ; China Agricultural University
- 年：2016
- 作者机构：中国农业大学饲料生物技术实验室动物营养学国家重点实验室;
- 论文关键词：细菌素 ; 抗生素 ; 抑菌活力 ; 热稳定性
- 英文论文关键词：bacteriocin ; antibiotics ; antibacterial activity ; thermal stability
- 会议召开时间：2016-11-04
- 会议录名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会论文集
- 语种：中文
- 分类号：Q936
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会
- 会议地点：中国山东青岛
- 主办单位：中国畜牧兽医学会动物微生态学分会
- 学会名称：中国畜牧兽医学会
- 页数：1
- 文件大小：64k
- 原文格式：O
- 会议级别：全国
摘要
为了初步研究实验室前期筛选获得的枯草芽孢杆菌产细菌素的生物学活性,试验采用杯碟扩散法绘制出硫酸黏杆菌素和杆菌肽锌两种抗生素的抑菌效价标准曲线,用抗生素效价当量来评价细菌素的活性,并在不同温度处理下测定其抗菌活性和热稳定性。结果表明,125 mg/mL细菌素溶液抑菌效价与26 mg/mL硫酸黏杆菌素或11.9 mg/mL杆菌肽锌溶液相当。此外,该细菌素在高温条件下(60~80℃左右)处理15 min仍能维持较高抑菌活性。综上所述,该试验在细菌素和抗生素的抑菌活力之间建立了更直观的联系,并证明了该细菌素具有较好的热稳定特性,为其产业化推广提供了理论支持和技术借鉴。
The standard curve of two antibiotics(colistin sulfate and bacitracin zinc) was carried out to assess antimicrobial activity of bacteriocin,which obtained from our earlier work,using cup-dish method.In addition,we also observed the effects of the reaction temperature on the antibacterial activity of the bacteriocin.Results showed that 125mg/mL bacteriocin displayed similar antibacterial activity to 26 mg/mL colistin sulfate or 11.9 mg/mL bacitracin zinc.Furthermore,the bacteriocin remained good thermal stability under high temperature(nearly 60~80℃) in 15 minutes.Overall,this research built a direct relationship between this bacteriocin and antibiotics,and testified good stablility of this bacteriocin,providing theoretical support and technology reference to promote its large scale production.
The standard curve of two antibiotics(colistin sulfate and bacitracin zinc) was carried out to assess antimicrobial activity of bacteriocin,which obtained from our earlier work,using cup-dish method.In addition,we also observed the effects of the reaction temperature on the antibacterial activity of the bacteriocin.Results showed that 125mg/mL bacteriocin displayed similar antibacterial activity to 26 mg/mL colistin sulfate or 11.9 mg/mL bacitracin zinc.Furthermore,the bacteriocin remained good thermal stability under high temperature(nearly 60~80℃) in 15 minutes.Overall,this research built a direct relationship between this bacteriocin and antibiotics,and testified good stablility of this bacteriocin,providing theoretical support and technology reference to promote its large scale production.
引文
[1]Galvin M,Hill C,Ross R P.Lacticin 3147 displays activity in buffer against gram-positive bacterial pathogens which appear insensitive in standard plate assays[J].Lett Appl Microbiol.1999,28(5):355-358.
[2]Goldstein B P,Wei J,Greenberg K,et al.Activity of nisin against Streptococcus pneumoniae,in vitro,and in a mouse infection model[J].J Antimicrob Chemother.1998,42(2):277-278.
[3]胡婷,陆文清.枯草芽孢杆菌MA139类细菌素抑菌活性的研究[J].中国畜牧杂志.2012(03):56-59.
[4]李振森.解淀粉芽孢杆菌Y-32.1产细菌素的研究[D].青岛科技大学,2014.
[2]Goldstein B P,Wei J,Greenberg K,et al.Activity of nisin against Streptococcus pneumoniae,in vitro,and in a mouse infection model[J].J Antimicrob Chemother.1998,42(2):277-278.
[3]胡婷,陆文清.枯草芽孢杆菌MA139类细菌素抑菌活性的研究[J].中国畜牧杂志.2012(03):56-59.
[4]李振森.解淀粉芽孢杆菌Y-32.1产细菌素的研究[D].青岛科技大学,2014.