摘要
An experiment was conducted to investigate the effect of arginine on feed intake regulation.One hundred and twenty six 1-day-old male White Pekin ducks(Anas platyrhynchos domestica) were randomly were allotted to one of two dietary treatments,birds were fed diets containing 0.71%(deficient) or 1.27%(sufficient) arginine for 3 weeks.At 21 days of age,feed intake were determined and hypothalamic protein profiles were analysed using isobaric tags for relative and absolute quantization technique.The birds fed with arginine-deficient diet had a lower final live body weight and cumulative feed intake(P<0.01) than those fed with arginine-sufficient diet.A total of 16 proteins were identified in hypothalamus with>l.5-fold expressional changes between arginine deficient and sufficient dietary treatments.Nine of these proteins were up-regulated and 7 of them were down-regulated.The identified proteins could be regrouped into 6 categories:protein processing,carbohydrate metabolism and energy production,transporter,cytoskeleton,immunity and neuronal development.Dietary arginine deficiency decreased expression of proteins involved in energy production(glycine amidinotransferase,aldolase B fructose-bisphosphate,aconitase,Transaldolase,6-phosphofructokinase type C-like) and oxygen transportation(hemoglobin subunit alpha expression).The proteomic alterations described here provides valuable insights into the interactions of arginine with appetite.
An experiment was conducted to investigate the effect of arginine on feed intake regulation.One hundred and twenty six 1-day-old male White Pekin ducks(Anas platyrhynchos domestica) were randomly were allotted to one of two dietary treatments,birds were fed diets containing 0.71%(deficient) or 1.27%(sufficient) arginine for 3 weeks.At 21 days of age,feed intake were determined and hypothalamic protein profiles were analysed using isobaric tags for relative and absolute quantization technique.The birds fed with arginine-deficient diet had a lower final live body weight and cumulative feed intake(P<0.01) than those fed with arginine-sufficient diet.A total of 16 proteins were identified in hypothalamus with>l.5-fold expressional changes between arginine deficient and sufficient dietary treatments.Nine of these proteins were up-regulated and 7 of them were down-regulated.The identified proteins could be regrouped into 6 categories:protein processing,carbohydrate metabolism and energy production,transporter,cytoskeleton,immunity and neuronal development.Dietary arginine deficiency decreased expression of proteins involved in energy production(glycine amidinotransferase,aldolase B fructose-bisphosphate,aconitase,Transaldolase,6-phosphofructokinase type C-like) and oxygen transportation(hemoglobin subunit alpha expression).The proteomic alterations described here provides valuable insights into the interactions of arginine with appetite.
引文