The Autophagy-related gene BcATG1 is Involved in Fungal Development and Pathogenesis in Botrytis cinerea
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- 英文论文题名：The Autophagy-related gene BcATG1 is Involved in Fungal Development and Pathogenesis in Botrytis cinerea
- 论文作者：REN Wei-chao ; ZHANG Zhi-hui ; SHAO Wen-yong ; YANG Ya-lan ; ZHOU Ming-guo ; CHEN Chang-jun
- 英文论文作者：REN Wei-chao ; ZHANG Zhi-hui ; SHAO Wen-yong ; YANG Ya-lan ; ZHOU Ming-guo ; CHEN Chang-jun ; College of Plant Protection ; Nanjing Agricultural University
- 年：2016
- 作者机构：College of Plant Protection,Nanjing Agricultural University;
- 英文论文关键词：Autophagy ; Botrytis cinerea ; BcATG1 ; Development ; Pathogenesis
- 会议召开时间：2016-08-05
- 会议录名称：中国植物病理学会2016年学术年会论文集
- 英文会议录名称：Proceedings of the Annual Meeting of Chinese Society for Plant Pathology(2016)
- 语种：英文
- 分类号：S432.44
- 学会代码：ZGVS
- 会议名称：中国植物病理学会2016年学术年会
- 会议地点：中国江苏南京
- 主办单位：中国植物病理学会
- 学会名称：中国植物病理学会
- 页数：1
- 文件大小：42k
- 原文格式：O
- 会议级别：全国
摘要
Autophagy,a ubiquitous intracellular degradation process,is conserved from yeast to human.It serves as a major survival function during nutrient depletion stress and is crucial for proper growth and differentiation.In this study,we characterized an atgl orthologue Bcatgl in the necrotrophic plant pathogen Botrytis cinerea.Quantitative real-time polymerase chain reaction(qRT-PCR) assays showed that the expression of BcATG1 was upregulated under carbon or nitrogen starvation conditions.BcATG1 can functionally restore the survival defects of the yeast ATG1 mutant during nitrogen starvation.Deletion of BcATG1(△Bcatg1) inhibited autophagosome accumulation in the vacuoles of nitrogen-starved cells.△Bcatg1 was dramatically impaired in vegetative growth,conidiation and sclerotial formation.Additionally,most conidia of △Bcatg1 lost the capacity to form the infection-structure appressorium and failed to penetrate onion epidermis.Pathogenicity assays showed that the virulence of △Bcatg1 on different host plant tissues was drastically impaired,which was consistent with its disability to form appressorium.Moreover,lipid droplets accumulation was significantly reduced in the conidia of △Bcatg1but glycerol contents was increased.All the defects of △Bcatg1 were complemented by reintroducing an intact copy of the wild-type BcATG1 into the mutant.These results indicate that BcATG1 plays a critical role in numerous developmental processes and is essential to pathogenesis of B.cinerea.
Autophagy,a ubiquitous intracellular degradation process,is conserved from yeast to human.It serves as a major survival function during nutrient depletion stress and is crucial for proper growth and differentiation.In this study,we characterized an atgl orthologue Bcatgl in the necrotrophic plant pathogen Botrytis cinerea.Quantitative real-time polymerase chain reaction(qRT-PCR) assays showed that the expression of BcATG1 was upregulated under carbon or nitrogen starvation conditions.BcATG1 can functionally restore the survival defects of the yeast ATG1 mutant during nitrogen starvation.Deletion of BcATG1(△Bcatg1) inhibited autophagosome accumulation in the vacuoles of nitrogen-starved cells.△Bcatg1 was dramatically impaired in vegetative growth,conidiation and sclerotial formation.Additionally,most conidia of △Bcatg1 lost the capacity to form the infection-structure appressorium and failed to penetrate onion epidermis.Pathogenicity assays showed that the virulence of △Bcatg1 on different host plant tissues was drastically impaired,which was consistent with its disability to form appressorium.Moreover,lipid droplets accumulation was significantly reduced in the conidia of △Bcatg1but glycerol contents was increased.All the defects of △Bcatg1 were complemented by reintroducing an intact copy of the wild-type BcATG1 into the mutant.These results indicate that BcATG1 plays a critical role in numerous developmental processes and is essential to pathogenesis of B.cinerea.
Autophagy,a ubiquitous intracellular degradation process,is conserved from yeast to human.It serves as a major survival function during nutrient depletion stress and is crucial for proper growth and differentiation.In this study,we characterized an atgl orthologue Bcatgl in the necrotrophic plant pathogen Botrytis cinerea.Quantitative real-time polymerase chain reaction(qRT-PCR) assays showed that the expression of BcATG1 was upregulated under carbon or nitrogen starvation conditions.BcATG1 can functionally restore the survival defects of the yeast ATG1 mutant during nitrogen starvation.Deletion of BcATG1(△Bcatg1) inhibited autophagosome accumulation in the vacuoles of nitrogen-starved cells.△Bcatg1 was dramatically impaired in vegetative growth,conidiation and sclerotial formation.Additionally,most conidia of △Bcatg1 lost the capacity to form the infection-structure appressorium and failed to penetrate onion epidermis.Pathogenicity assays showed that the virulence of △Bcatg1 on different host plant tissues was drastically impaired,which was consistent with its disability to form appressorium.Moreover,lipid droplets accumulation was significantly reduced in the conidia of △Bcatg1but glycerol contents was increased.All the defects of △Bcatg1 were complemented by reintroducing an intact copy of the wild-type BcATG1 into the mutant.These results indicate that BcATG1 plays a critical role in numerous developmental processes and is essential to pathogenesis of B.cinerea.
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