红鳍东方鲀转录因子GATA4基因的表达
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- 英文论文题名：The expression of transcription factor GATA4 gene in Takifugu rubripes
- 论文作者：郝薇薇 ; 仇雪梅 ; 高长富 ; 王秀利
- 英文论文作者：Hao Weiwei ; Qiu Xuemei ; Gao Changfu ; Wang Xiuli ; College of Fisheries and Life Science ; Dalian Ocean University
- 年：2016
- 作者机构：大连海洋大学水产与生命学院;
- 论文关键词：红鳍东方鲀 ; 实时定量PCR ; 转录因子GATA4 ; 基因表达
- 英文论文关键词：Takifugu rubripes ; quantitative Real-time PCR ; transcription factor GATA4 ; gene expression
- 会议召开时间：2016-11-02
- 会议录名称：2016年中国水产学会学术年会论文摘要集
- 语种：中文
- 分类号：S917.4
- 学会代码：OGSB
- 会议名称：2016年中国水产学会学术年会
- 会议地点：中国四川成都
- 主办单位：中国水产学会、四川省水产学会
- 学会名称：中国水产学会
- 页数：1
- 文件大小：561k
- 原文格式：D
- 会议级别：全国
摘要
利用荧光实时定量PCR(qRT-PCR)技术对红鳍东方鲀(Takifugu rubripes)成鱼的肾脏、肌肉、脑、脾脏、肝脏、精巢和卵巢等组织进行了GATA4基因的表达分析,并对幼鱼不同发育时间的个体水平上的GATA4基因的表达进行了分析。结果显示,红鳍东方鲀成鱼的肌肉、脾脏、肾脏和脑等4个组织中的GATA4的表达量差异不显,精巢、卵巢和肝脏的GATA4的表达量显著高于其他组织的表达量(P<0.05),GATA4在精巢、肝脏中的表达量显著高于在卵巢中的表达量(P<0.05)。在雄性幼鱼5个发育时期中(23、30、40、60、80日龄),GATA4的表达量从23日龄到30日龄逐渐升高,30日龄最高且明显高于同时期雌鱼GATA4的表达量(P<0.01),随后到40日龄之间表达量逐渐降低,从60日龄到80日龄又有上升的趋势。在雌性幼鱼5个发育时期中,GATA4表达量从23日龄到40日龄逐渐升高,40日龄最高且明显高于同时期雄鱼GATA4的表达量(P<0.01),到60日龄期间表达量降低,从80日龄后又有上升的趋势。
In this study, GATA4 expression were analyzed in kidney, muscle, brain, spleen, liver,testis and ovary of mature Takifugu rubripes, and were analyzed at individual level during different times in young T.rubripes by flurescence quantitative Real-time Polymerase Chain Reaction(qRT-PCR). The results showed that no statistically significant difference was observed of expression level among muscle, spleen, kidney and brain. There was the significantly higher expression of GATA4 in testis, liver and ovary, than that in other organs(P<0.05). The GATA4 expression level of testis and liver were significantly higher(P<0.05), than that in ovary. During the different periods(23,30,40,60,80 days of age(da)) of T. rubripes, the GATA4 expression level in males increased gradually from 23 da to 30 da and subsequently decreased at 40 da, and the 30 da expression level of GATA4 was higher than that in 30 da female individuals significantly(P<0.01).While from 60 da to 80 da, the GATA4 expression level went steadily up. In females, as the same as the expression tend in males, the GATA4 expression level increased gradually from 23 da to 40 da and subsequently decreased at 60 da, and the 40 da GATA4 gene expressed higher than that in the same time male individuals significantly(P<0.01).
In this study, GATA4 expression were analyzed in kidney, muscle, brain, spleen, liver,testis and ovary of mature Takifugu rubripes, and were analyzed at individual level during different times in young T.rubripes by flurescence quantitative Real-time Polymerase Chain Reaction(qRT-PCR). The results showed that no statistically significant difference was observed of expression level among muscle, spleen, kidney and brain. There was the significantly higher expression of GATA4 in testis, liver and ovary, than that in other organs(P<0.05). The GATA4 expression level of testis and liver were significantly higher(P<0.05), than that in ovary. During the different periods(23,30,40,60,80 days of age(da)) of T. rubripes, the GATA4 expression level in males increased gradually from 23 da to 30 da and subsequently decreased at 40 da, and the 30 da expression level of GATA4 was higher than that in 30 da female individuals significantly(P<0.01).While from 60 da to 80 da, the GATA4 expression level went steadily up. In females, as the same as the expression tend in males, the GATA4 expression level increased gradually from 23 da to 40 da and subsequently decreased at 60 da, and the 40 da GATA4 gene expressed higher than that in the same time male individuals significantly(P<0.01).
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